Adult male and female Sprague Dawley (Taconic Biosciences) rats (n = 3 of each sex for each route) were used. Nano-dezocine [9 ] was administered either intraperitoneally (IP) (2.0 mg/kg in a volume of 1 ml/kg) or intranasally (IN) (2.0 mg/kg in a maximum volume of 75 μl/kg total for both nostrils). At 15, 30, 60, 120, 240 and 480 minutes after administration, rats were lightly anesthetized with isoflurane and blood was drawn (approximately 200 μl) from the ventral tail vein. Blood was placed into heparinized tubes, centrifuged, and plasma was collected and frozen. Dezocine was measured by Liquid Chromatography-Mass Spectroscopy (LC-MS) using a Thermo Scientific Q-Exactive HF-X mass spectrometer coupled to the Thermo Scientific Vanquish Horizon UHPLC system. Dezocine was quantified using the full MS data by extracted ion chromatogram (XIC) of 246.1852 m/z at 5 ppm mass tolerance and the accurate retention time. Quantitative recovery of dezocine was obtained using ice cold 80% methanol as the extraction solvent in experimental plasma samples.
Vanquish horizon uhplc system
Manufactured by Thermo Fisher Scientific
Sourced in United States, Germany
The Vanquish Horizon UHPLC system is a high-performance liquid chromatography (HPLC) instrument designed for ultra-high-performance liquid chromatography (UHPLC) applications. It provides advanced technology for efficient separation, detection, and quantification of complex samples. The system is capable of operating at high pressures and flow rates to deliver rapid, high-resolution separations.
Automatically generated - may contain errors
Lab products found in correlation
Q exactive hf x mass spectrometer, by Thermo Fisher Scientific (7 mentions)
Xbridge beh amide column, by Waters Corporation (4 mentions)
Hypersil gold c18 column, by Thermo Fisher Scientific (3 mentions)
Tracefinder 4, by Thermo Fisher Scientific (3 mentions)
Acquity uplc hss t3 column, by Waters Corporation (2 mentions)
Zic philic 2.1 mm i d 150 mm column, by Merck Group (2 mentions)
Synergi 4mm polar rp 2 mm i d 100 mm column, by Phenomenex (2 mentions)
Tsq quantis triple quadrupole, by Thermo Fisher Scientific (2 mentions)
Q exactive hf, by Thermo Fisher Scientific (2 mentions)
Infinitylab poroshell 120 ec c18 column, by Agilent Technologies (2 mentions)
Optima lc ms grade, by Thermo Fisher Scientific (2 mentions)
Pierce ltq velos esi, by Thermo Fisher Scientific (1 mentions)
Q exactive plus orbitrap, by Thermo Fisher Scientific (1 mentions)
Ltq orbitrap velos mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Accucore 150 amide hilic lc column, by Thermo Fisher Scientific (1 mentions)
Acquity uplc hss c18 column, by Waters Corporation (1 mentions)
Acquity uplc t3 column, by Waters Corporation (1 mentions)
Orbitrap id x tribrid mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Accucore c18 column, by Thermo Fisher Scientific (1 mentions)
Amicon ultra 0.5 filter devices, by Merck Group (1 mentions)
38 protocols using vanquish horizon uhplc system
1
Pharmacokinetics of Intranasal Nano-dezocine
2
High-Resolution LC-MS Analysis of Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
High-resolution LC–MS analysis was performed on a Thermo Fisher Scientific Vanquish Horizon UHPLC System coupled with a Thermo Q Exactive hybrid quadrupole-orbitrap high-resolution mass spectrometer equipped with a HESI ion source. One microliter of extract was injected and separated using a water-acetonitrile gradient on a Thermo Scientific Hypersil GOLD C18 column (150 mm × 2.1 mm 1.9 µm particle size 175 Å pore size, Thermo Scientific) and maintained at 40°C. Solvents were all purchased from Fisher Scientific as HPLC grade. Solvent A: 0.1% formic acid in water; solvent B: 0.1% formic acid in acetonitrile. A/B gradient started at 1% B for 3 min, then from 1% to 100% B over 20 min, 100% for 5 min, then down to 1% B for 3 min. Mass spectrometer parameters: 3.5 kV spray voltage, 380°C capillary temperature, 300°C probe heater temperature, 60 sheath flow rate, 20 auxiliary flow rate, 2.0 spare gas; S-lens RF level 50.0, resolution 240,000, m/z range 150–1,000 m/z, AGC target 3e6. The instrument was calibrated with positive and negative ion calibration solutions (Thermo-Fisher) Pierce LTQ Velos ESI pos/neg calibration solutions. Peak areas were determined using Xcalibur 2.3 QualBrowser version 2.3.26 (Thermo Scientific) using a 5-ppm window around the m/z of interest.
3
HILIC Separation of Isotopically Labeled Compounds
4
UHPLC Gradient Separation Optimization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid chromatography was performed using a Vanquish Horizon UHPLC system by Thermo Fisher (Waltham, MA, USA) build from a binary pump H, a split sampler HT, and a temperature-controlled column compartment. Chromatographic separation was achieved on an ACQUITY UPLC HSS T3 Column (100 Å, 1.8 μm, 2.1 ×100 mm, Waters, Milford, USA) at 40°C with a flowrate of 0.5 mL/min using H2O + 0.1% HCOOH as eluent A and CH3CN + 0.1% HCOOH as eluent B. The following gradient was applied: (i) 4% B isocratic from 0.0–0.5 min; (ii) linear increase to 15% B until 4 min; (iii) linear increase to 80% until 7.0 min; (iv) linear increase to 100% until 7.1 min; (v) holding 100% B until 10.0 min; (vi) back to the starting conditions of 4% B until 10.1 min; and (vii) equilibration for 4.5 min until the next run.
5
Quantitative Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analysis by UHPLC-HESI-HRMS provided
product separation and accurate mass measurements using a Q Exactive
Plus Orbitrap high-resolution mass spectrometer coupled to a Vanquish
Horizon UHPLC system (Thermo Scientific). The UHPLC system allowed
for the separation of products, and the molecular formula of each
product was obtained from high-resolution MS data. Full scans in ESI
(−) ion mode with the range 100–500 amu were used. The
detailed parameters of UHPLC-HESI-HRMS can be found inText S1 .
product separation and accurate mass measurements using a Q Exactive
Plus Orbitrap high-resolution mass spectrometer coupled to a Vanquish
Horizon UHPLC system (Thermo Scientific). The UHPLC system allowed
for the separation of products, and the molecular formula of each
product was obtained from high-resolution MS data. Full scans in ESI
(−) ion mode with the range 100–500 amu were used. The
detailed parameters of UHPLC-HESI-HRMS can be found in
6
Indigoidine production and extraction protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
100 μl of streptomycetes spore suspensions were inoculated into 10 ml YEME media and incubated at 28oC overnight in shaking incubator, 200 rpm, to produce seed cultures. Next day 2.5 ml of seed cultures were used to start 25 ml fermentation in YEME media, at the same incubation conditions. The blue color indicating the expression of bspA gene appeared after 3–4 days of incubation. Spectrophotometric indigoidine detection was done as described previously (24 (link)) using culture supernatant. Fermentation continued for 7 days, and then the whole fermentation cultures were quickly frozen in a cold ethanol bath and lyophilized.
25 ml of methanol was added to the lyophilized material. The suspensions were processed on rotary shaker at room temperature for 60 min, and then centrifuged at 8000 rpm for 10 min. Supernatants were dried completely using rotavapors. The dry material was dissolved in 1 ml of methanol, and 100 μl of extracts were used for MS/MS analysis.
LC–MS analyses were performed on a Vanquish Horizon UHPLC system (Thermo Fisher Scientific) coupled to the ESI source of an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). Chromatographic and MS parameters were used as described previously (25 (link)), except that MS1 spectra were recorded in a range of m/z 150–1000.
25 ml of methanol was added to the lyophilized material. The suspensions were processed on rotary shaker at room temperature for 60 min, and then centrifuged at 8000 rpm for 10 min. Supernatants were dried completely using rotavapors. The dry material was dissolved in 1 ml of methanol, and 100 μl of extracts were used for MS/MS analysis.
LC–MS analyses were performed on a Vanquish Horizon UHPLC system (Thermo Fisher Scientific) coupled to the ESI source of an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). Chromatographic and MS parameters were used as described previously (25 (link)), except that MS1 spectra were recorded in a range of m/z 150–1000.
7
LC-MS Analysis of Isoprenoid Precursors
8
Pharmacokinetics of Intranasal Nano-dezocine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult male and female Sprague Dawley (Taconic Biosciences) rats (n = 3 of each sex for each route) were used. Nano-dezocine [9 ] was administered either intraperitoneally (IP) (2.0 mg/kg in a volume of 1 ml/kg) or intranasally (IN) (2.0 mg/kg in a maximum volume of 75 μl/kg total for both nostrils). At 15, 30, 60, 120, 240 and 480 minutes after administration, rats were lightly anesthetized with isoflurane and blood was drawn (approximately 200 μl) from the ventral tail vein. Blood was placed into heparinized tubes, centrifuged, and plasma was collected and frozen. Dezocine was measured by Liquid Chromatography-Mass Spectroscopy (LC-MS) using a Thermo Scientific Q-Exactive HF-X mass spectrometer coupled to the Thermo Scientific Vanquish Horizon UHPLC system. Dezocine was quantified using the full MS data by extracted ion chromatogram (XIC) of 246.1852 m/z at 5 ppm mass tolerance and the accurate retention time. Quantitative recovery of dezocine was obtained using ice cold 80% methanol as the extraction solvent in experimental plasma samples.
9
HILIC-Based N-Glycan Separation and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Labeled N-glycans were separated with Thermo Scientific Accucore 150 Amide HILIC LC Column (2.6 μm, 150 A, 2.1 × 150 mm) at 50°C with Thermo Scientific Vanquish Horizon UHPLC System equipped with 2 μl flow cell FLD at 455 nm and 500 nm excitation and emission wavelength, respectively, at high power lamp mode and 10 Hz of data collection rate. Separation was performed with 15 μl injection volume at a 45 min gradient of B from 32% to 45%, 45.5–47 min at 60%, and 47.5–50 min at 32% at constant flow rate of 0.45 ml/min, with mobile phase B being 100 mM ammonium formate (pH 4.4) and mobile phase A being 100% acetonitrile.
10
UHPLC Separation of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid-chromatography was performed on a Vanquish Horizon UHPLC System by Thermo Fisher (Waltham, MA, USA) build from a Vanquish binary pump H, a Vanquish split sampler HT and a temperature-controllable Vanquish column compartment. Chromatographic separation was achieved at 25 °C on an ACQUITY UPLC HSS C18 Column (100 Å, 1.8 µm, 2.1 × 100 mm, Waters, Milford, USA). Eluent A consisted of H2O + 0.1% HCOOH and B of CH3CN + 0.1% HCOOH. The following gradient was applied at a constant flowrate of 0.4 mL: (i) 4% B isocratic from 0.0 to 0.5 min; (ii) linear increase to 11% B until 5.25 min; (iii) linear increase to 95% until 7.0 min; (iv) holding 95% B until 10.0 min (vi) back to the starting conditions of 4% B until 10.5 min; (vii) equilibration for 4.5 min until the next run. The injection volume amounted 5 µL.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!