Egf1 53

Manufactured by Thermo Fisher Scientific

Sourced in United States

EGF1-53 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for specific research and experimental applications. The core function of EGF1-53 is to provide a precise and controlled environment for conducting experiments and analyses. No further details are available without potentially making unsubstantiated claims.

Automatically generated - may contain errors

Lab products found in correlation

Bovine pituitary extract, by Thermo Fisher Scientific (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Pbs buffer, by Thermo Fisher Scientific (2 mentions) Keratinocyte sfm medium, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Recombinant human tgf β1, by R&D Systems (1 mentions) Eagle s minimum essential medium, by Merck Group (1 mentions) Beas 2b, by American Type Culture Collection (1 mentions) Penicillin streptomycin solution, by Merck Group (1 mentions) Penicillin streptomycin solution, by Cytiva (1 mentions) Dmem media, by Cytiva (1 mentions) Rpmi media, by Cytiva (1 mentions) Liberase, by Roche (1 mentions) Azd6244, by Selleck Chemicals (1 mentions) Rpmi 1640, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Dulbecco s modified eagle s medium, by Merck Group (1 mentions) Penicillin, by Biowest (1 mentions)

6 protocols using egf1 53

Extracellular lncRNA Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were obtained from Shanghai Institutes for Biological Sciences (Shanghai, China). 6-10B, 5-8F, CNE1, CNE2 and B95-8 cells were cultured in DMEM (Gibco) or RPMI 1640 medium (Gibco) containing 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin at 37°C in a humidified environment with 5% CO₂. NP69 cell was cultured in the Keratinocyte-SFM medium which contained epidermal growth factor 1-53 (EGF1-53) and bovine pituitary extract (BPE) (Gibco). Supernatants were collected for measurement of extracellular lncRNAs 24 h, 48 h and 72 h after subculture. Culture supernatants were purified by centrifugation at 2,000 g for 15 min to remove cell remnants. The sequences of the AL359062 siRNA1 and siRNA2 were 5’-GCACAAATCAAAGGCCAAT-3’ and 5’-GCCTGACACCTTCCCGATAC-3’ respectively, which were provided by TaKaRa.

He B., Zeng J., Chao W., Chen X., Huang Y., Deng K., Huang Z., Li J., Dai M., Chen S., Huang H, & Dai S. (2017). Serum long non-coding RNAs MALAT1, AFAP1-AS1 and AL359062 as diagnostic and prognostic biomarkers for nasopharyngeal carcinoma. Oncotarget, 8(25), 41166-41177.

+ Open protocol

+ Expand

Culturing Bronchial Epithelial Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human bronchial epithelial cell line BEAS-2B was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in serum-free Bronchial Epithelial Cell Growth Medium (BEGM) medium (Lonza, Walkersville, MD, USA), supplemented with Bronchial Epithelial Cell Growth Medium SingleQuots™ Supplements and Growth Factors (Lonza). The HBEC-3KT bronchial epithelial cell line was kindly provided by Professor Jerry Shay (UT Southwestern, TX, USA). The cells were immortalized in the laboratory of Prof. Shay and further cultured as described by Ramirez et al. [29 (link)]. In brief, the cells were cultured in KSF (Keratinocyte Serum-Free) media (Gibco, Carlsbad, CA, USA) supplemented with epidermal growth factor 1-53 (EGF 1-53) and Bovine Pituitary Extract (BPE) provided frozen from the manufacturer. The MRC-9 normal human lung fibroblasts were purchased from the ATTC. The cells were expanded and maintained in Eagle's Minimum Essential Medium (Sigma-Aldrich, St. Louise, MO, USA) supplemented with fetal bovine serum and L-glutamine as instructed by the supplier.
The cells were stimulated with 0.1-0.2 ng/ml recombinant human TGF-β1 (#240-B-002, R&D Systems, Minneapolis, USA).

Acheva A., Haghdoost S., Sollazzo A., Launonen V, & Kämäräinen M. (2019). Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma Radiation. Oxidative Medicine and Cellular Longevity, 2019, 4120379.

+ Open protocol

+ Expand

Establishing Human Pancreatic Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human PC cell lines SW1990, CD18, Capan1, and the human pancreatic ductal cell line HPDE were obtained from American type culture collection (Manassas, VA, USA). SW1990, CD18, and Capan1 cells were cultured in DMEM media (HyClone Laboratories, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich, St Louis, MO, USA) and 1% penicillin-streptomycin solution (Sigma). HPDE was cultured in human keratinocyte serum-free media supplemented with epidermal growth factor 1–53 (EGF 1–53) and bovine pituitary extract (BPE) (Gibco). Normal human fibroblast cell line 9–26 NP was derived from surgically obtained cancer-associated normal tissue. Briefly, the tissue was finely chopped and digested with Liberase (Roche), and cultured in RPMI media (HyClone Laboratories, Logan, UT, USA) supplemented with 1% HEPES, 1% sodium pyruvate, 1% L-glutamine, 1% sodium bicarbonate, 1% non-essential amino acids, and 1% penicillin-streptomycin solution. These cells were subjected to differential trypsinization up to 7 passages, immortalized using hTERT, and then selected using puromycin to generate a stable fibroblast cell line. Cells were incubated in a humidified incubator at 37 °C and supplied with 5% CO₂. Cells were subcultured by trypsin-EDTA treatment, and complete medium was changed every other day.

Karmakar S., Rauth S., Nallasamy P., Perumal N., Nimmakalaya R.K., Leon F., Gupta R., Barkeer S., Venkata R.C., Raman V., Rachagani S., Ponnusamy M.P, & Batra S.K. (2020). PAF1 Regulates Stem Cell Features of Pancreatic Cancer Cells, Independently of the PAF1 Complex, via Interactions with PHF5A and DDX3. Gastroenterology, 159(5), 1898-1915.e6.

+ Open protocol

+ Expand

Cell Line Culture and Drug Treatment Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

A549, H460, H358, H441, and H2122 cell lines were obtained from the American Type Culture Collection. HBEC (HBEC3-KT) immortalized by CDK4 and hTERT was described by Ramirez and colleagues (27 (link)). Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) was used for A549 and RPMI 1640 (Sigma-Aldrich) for H460, H358, H441, and H2122. All media contained 10% fetal bovine serum (Sigma-Aldrich), 2 mM L-glutamine (Sigma-Aldrich), penicillin (50 IU/ml; Biowest), and streptomycin (50 μg/ml; Biowest). HBEC cells were cultured in keratinocyte serum-free growth medium supplemented with EGF 1–53 and bovine pituitary extract (Thermo Fisher Scientific). SMAP (DT-061) was manufactured as described in (11 (link)). AZD6244 was purchased from Selleck Chemicals, and all the other drugs were from MedChemExpress. The drugs were diluted in dimethyl sulfoxide and stored at room temperature (DT-061) or −20°C (all other drugs).

Kauko O., O’Connor C.M., Kulesskiy E., Sangodkar J., Aakula A., Izadmehr S., Yetukuri L., Yadav B., Padzik A., Laajala T.D., Haapaniemi P., Momeny M., Varila T., Ohlmeyer M., Aittokallio T., Wennerberg K., Narla G, & Westermarck J. (2018). PP2A inhibition is a druggable MEK inhibitor resistance mechanism in KRAS-mutant lung cancer cells. Science translational medicine, 10(450), eaaq1093.

+ Open protocol

+ Expand

Chronic Acrolein Exposure Alters Normal Oral Keratinocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human normal oral keratinocyte (NOK) was kindly gifted by Dr. Kuo-Wei Chang at the Institute of Oral Biology, School of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan, and was authenticated [31 (link)]. NOK cells were grown in keratinocyte-SFM (KFSM, 1X, Thermo Fisher Scientific, Waltham, MA, USA) medium supplemented with human recombinant 0.2 ng/mL EGF 1–53 and 25 µg/mL bovine pituitary extract Thermo Fisher Scientific, Waltham, MA, USA). Acrolein (Acr) stock solution (Sigma–Aldrich, St. Louis, MO, USA) was freshly prepared before use. Cells at 70% confluency were washed with PBS buffer (Thermo Fisher Scientific, Waltham, MA, USA) and treated with acrolein (7.5 μM) in complete culture medium for 1 month at 37 °C in the dark and acrolein-containing medium was changed every other days.

Tsou H.H., Tsai H.C., Chu C.T., Cheng H.W., Liu C.J., Lee C.H., Liu T.Y, & Wang H.T. (2021). Cigarette Smoke Containing Acrolein Upregulates EGFR Signaling Contributing to Oral Tumorigenesis In Vitro and In Vivo. Cancers, 13(14), 3544.

+ Open protocol

+ Expand

Culturing HEK-293, RWPE-1, and PrEC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK-293 cells and prostate benign epithelial cells (RWPE-1, #CRL-11609) were purchased from American Type Culture Collection (Manassas, VA). Primary prostate epithelial cells (PrEC) were purchased from Lonza (Walkersville, MD). HEK-293 cells were cultured in MEM media (Thermo Fisher Scientific, catalog #11095080,) supplemented with 10% FBS (fetal bovine serum, Thermo Fisher Scientific, catalog number #10082147). RWPE-1 cells were cultured in keratinocyte serum free medium (K-SFM, Gibco, Thermo Fisher Scientific, catalog #17005-042, Carlsbad, CA) supplemented with Bovine Pituitary Extract (BPE, 0.05 mg/ml, Thermo Fisher Scientific, catalog #17005-042), human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042), and 1% penicillin/streptomycin. PrEC cells were cultured in Prostate Epithelial Cell Basal Medium (PrEGM) supplemented with Prostate Epithelial Cell Growth Kit (Clonetics PrEGM, BulletKit, Lonza). All cell cultures were maintained at 37 °C in an incubator with a controlled humidified atmosphere composed of 95% air and 5% CO₂.

Chakravarthi B.V., Dedigama-Arachchige P., Carskadon S., Sundaram S.K., Li J., Wu K.H., Chandrashekar D.S., Peabody J.O., Stricker H., Hwang C., Chitale D.A., Williamson S.R., Gupta N.S., Navone N.M., Rogers C., Menon M., Varambally S, & Palanisamy N. (2019). Pseudogene Associated Recurrent Gene Fusion in Prostate Cancer. Neoplasia (New York, N.Y.), 21(10), 989-1002.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!