Amphotericin

Manufactured by HiMedia

Sourced in India, United States

Amphotericin is a type of antifungal medication used in laboratory settings. It functions as a broad-spectrum antifungal agent effective against a variety of fungal species.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Penicillin, by HiMedia (3 mentions) Trypsin, by HiMedia (2 mentions) Ethylenediaminetetraacetic acid, by HiMedia (2 mentions) Streptomycin, by HiMedia (2 mentions) Fetal bovine serum (fbs), by HiMedia (2 mentions) Penicillin streptomycin, by HiMedia (2 mentions) Dulbecco s modified eagle s medium, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions) H9c2 cell line, by American Type Culture Collection (1 mentions) Antibiotic solution of penicillin streptomycin, by Merck Group (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Teicoplanin, by HiMedia (1 mentions) Cefoperazone, by HiMedia (1 mentions) Uv 1800 spectrophotometer, by Shimadzu (1 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions) Mda mb 231, by National Centre for Cell Science (1 mentions)

9 protocols using amphotericin

HeLa Cell Culture Maintenance Protocol

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The cell
line used in the present study is human cervical cancer cell line,
HeLa cells, obtained from National Centre for Cell Science, Pune,
India. For maintenance of cell lines, Dulbecco’s modified Eagle’s
medium (Sigma-Aldrich) containing 10% fetal bovine serum (Gibco),
antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin),
and amphotericin (0.25 μg/mL) (HiMedia) were employed. The cells
were maintained in CO₂ incubators at 37 °C with 5%
CO₂ in air and 99% humidity. The passaging of cells when
confluent was carried out using 0.25% trypsin and 0.02% ethylenediaminetetraacetic
acid (HiMedia) in phosphate-buffered saline. Experiments were carried
out after 36 h of seeding the cells at appropriate density in suitable
well plates.

Nair R.V., Thomas R.T., Sankar V., Muhammad H., Dong M, & Pillai S. (2017). Rapid, Acid-Free Synthesis of High-Quality Graphene Quantum Dots for Aggregation Induced Sensing of Metal Ions and Bioimaging. ACS Omega, 2(11), 8051-8061.

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Isolating Fungal Strains from Oil Refinery Soil

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To isolate fungal strain, the samples were isolated from soil around oil refinery. 1 g of sample was dissolved in 10 mL of sterilized distilled water, serially diluted upto 10⁻⁶ and plated onto Potato Dextrose Agar (PDA) medium containing (component g/L) potato infusion (infusion from 200 g potatoes), 4; dextrose, 20; agar, 15. After sterilization, the medium was supplemented with 10 μg amphotericin/mL and 25 μg streptomycin/mL (Himedia, Mumbai, India) to inhibit fungal and bacterial contamination respectively. The medium was incubated at 35 °C for 72 h. One plate was kept as an uninoculated control. Pure colonies were isolated by subculturing on PDA. Stock cultures were maintained at 4 °C. Colonies having zone formation were subcultured in potato dextrose broth. The spore morphology was determined by light microscopy and scanning electron microscopy.

Sakthiselvan P., Naveena B, & Partha N. (2015). Molecular characterization of a Xylanase-producing fungus isolated from fouled soil. Brazilian Journal of Microbiology, 45(4), 1293-1302.

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Culturing Human Hepatocellular Carcinoma Cells

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Human hepatocellular carcinoma cell line (Huh-7) was procured form National centre for cell sciences (NCCS), Pune. Huh-7 cells were maintained in T-25 culture flasks and 30 mm Petri dishes, containing Dulbecco's Modified Eagle Medium (DMEM) with 4.5 g L⁻¹ glucose and l-glutamine (Lonza, USA) supplemented with 10% fetal bovine serum (Himedia) and 1% of antibiotics (100 U mL⁻¹ penicillin, 100 μg mL⁻¹ of streptomycin, 0.25 μg mL⁻¹ of amphotericin, Himedia) was incubated in 5% CO₂ humidified atmosphere at 37 °C.

Sathiya Savithri J, & Rajakumar P. (2019). Synthesis, optical, electrochemical properties and anticancer activity of (S)-BINOL cored triazole bridged dendrimers decorated with rhodamine B surface group. RSC Advances, 9(63), 36994-37002.

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Maintenance of H9c2 Cardiomyoblast Cell Lines

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H9c2 cell lines
(rat embryonic cardiomyoblasts) obtained from American Type Culture
Collection (ATCC). For the maintenance of cell lines, Dulbecco’s
modified Eagle’s medium (DMEM) (Sigma-Aldrich) containing 10%
fetal bovine serum (Gibco), antibiotics (100 U/mL penicillin and 100
μg/mL streptomycin), and amphotericin (0.25 μg/mL) (HiMedia)
was employed. The cells were maintained in cell culture flasks in
CO₂ incubators at 37 °C with 5% CO₂ in
air and 99% humidity. Passaging of cells when confluent was carried
out using 0.25% trypsin and 0.02% ethylenediaminetetraacetic acid
(HiMedia) in phosphate-buffered saline.

Nagarajan S., Sankar V., Bejoymohandas K.S., Duan Y, & Zhang J. (2018). Influence of Branched Polyester Chains on the Emission Behavior of Dipyridamole Molecule and Its Biosensing Ability. ACS Omega, 3(11), 15530-15537.

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Culturing Pancreatic and Fibroblast Cell Lines

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Pancreatic carcinoma MIA PaCa-2 cell line was procured from National Centre for Cell Science (NCCS), Pune, India and Human dermal fibroblast HDF cell line was procured from V.G. Vaze College, Mumbai, India. Both the cell lines were cultured in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma–Aldrich, St. Louis, MO, USA) with heat inactivated 10% foetal bovine serum (FBS) (Cell Clone, Genetix, India) and 1% antibiotic solution of penicillin–streptomycin (Sigma–Aldrich, St. Louis, MO, USA), and amphotericin (Himedia, Mumbai, India). Cells were maintained by passaging 1:3 at a regular interval of 48 h using 0.5% Trypsin-EDTA (Gibco, Paisley, UK) without phenol red. All reagents, media, buffers, chemicals and plastic-ware used were of cell culture grade.

Chhabria S, & Desai K. (2016). Purification and characterisation of alliinase produced by Cupriavidus necator and its application for generation of cytotoxic agent: Allicin. Saudi Journal of Biological Sciences, 25(7), 1429-1438.

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Isolation of Campylobacter jejuni from Broiler Chickens

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To isolate C. jejuni, the cecal content of commercial broiler chickens was collected and processed (Singh and Mallick, 2019 (link)). Processed samples were plated onto a blood Free Campylobacter Selectivity Agar Base medium (HiMedia, India) supplemented with CAT selective supplement (cefoperazone 8 mg/L, amphotericin 10 mg/L, and teicoplanin 4 mg/L) (HiMedia, India) and incubated for 36-48 h at 42°C. Colonies were examined for characteristic morphological appearance followed by biochemical analysis including catalase, oxidase, and hippurate hydrolysis test. Further, selected colonies were checked for the presence of 16S rRNA and other major genes encoding virulence factors (GEVFs), including hipO, cadF, ciaB genes.
The standard C. jejuni strain (NCTC 11168; Genbank ID: AL111168.1) was obtained through the NIH Biodefense and Emerging Infections Research Repository, NIAID, NIH: Campylobacter jejuni subsp. jejuni, Strain NCTC 11168, NR-126.

Gupta S., Ray S., Khan A., China A., Das D, & Mallick A.I. (2021). The cost of bacterial predation via type VI secretion system leads to predator extinction under environmental stress. iScience, 24(12), 103507.

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Evaluation of Anti-inflammatory Potential

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Triton X 100, amphotericin (5 mg/mL), and gentamycin (4 mg/mL) were purchased from HiMedia Laboratories Pvt. Ltd., Mumbai, India. Fetal bovine serum (FBS), Pen Strep (a mixture of penicillin and streptomycin), and Dulbecco's modified Eagle medium (DMEM) were procured from Gibco Life Technologies (Bangalore, India); MTT (methylthiazolyl diphenyl-tetrazolium bromide), DMSO (dimethyl sulfoxide), Gallic acid, Quercetin, FeSO₄·7H₂O, F–C reagent (Folin Ciocalteu), 2,4,6-Tris (2-pyridyl)-1,3,5-triazine (TPTZ) were purchased from HiMedia Laboratories Pvt. Ltd. The cancer cell line MDA-MB-231 and HT1080 was obtained from National Centre for Cell Science (NCCS), Pune, Maharashtra, India. All other chemicals used were of analytical grade. UV–Visible spectrophotometer (SHIMADZU spectrophotometer UV-1800) was used for detection of absorbance. The anti-inflammatory activity was assessed using electrophoresis assembly (BioBee Tech, Bangalore) and gel documentation (Syngene, G Box, United Kingdom).

Kulkarni S.S., Mishra S., Patil S.B., Nambiar J, & Math A. (2021). Unifloral ajwain honey ameliorates differential inhibition of matrix metalloproteinases 2 and 9 protein, cytotoxicity, and antioxidant potential. Journal of Ayurveda and Integrative Medicine, 12(4), 633-639.

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Culturing Breast Cancer Cell Lines

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MDA-MB-231 and SKBR3 cells were maintained in DMEM/F12 (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 0.1% penicillin-streptomycin (Hi-Media, Mumbai, Maharashtra, India), 0.1% amphotericin (Hi-Media, Mumbai, Maharashtra, India), and 0.1% ciprofloxacin (Ranbaxy Laboratories Ltd., Gurugram, Haryana, India) in humidified incubator with 5% CO₂ at 37 °C. MCF7 cells were maintained in DMEM (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1% penicillin-streptomycin (Hi-Media, Mumbai, Maharashtra, India) in a humidified incubator with 5% CO₂ at 37 °C. Cell lines used in the study were tested for absence of Mycoplasma contamination and validated by morphological observation. All the experiments were performed post 24 h of cell seeding unless otherwise stated.

Lohiya G, & Katti D.S. (2021). A Synergistic Combination of Niclosamide and Doxorubicin as an Efficacious Therapy for All Clinical Subtypes of Breast Cancer. Cancers, 13(13), 3299.

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Corneal Stromal Cells: Sodium Chlorate Effects

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Corneal stromal cells were cultured on coverslips in 24-well plates with an initial seeding density of 1 × 10 5 cells/well. Alpha MEM with 10% fetal bovine serum (Himedia Laboratories, Mumbai, India), 1% penicillin-streptomycin (Himedia Laboratories), 1% amphotericin (Himedia Laboratories), 0.5% gentamicin (Himedia Laboratories), and 1% HEPES (Himedia Laboratories) was used for culturing the cells. Sodium chlorate (Sigma-Aldrich, Bengaluru, India) was added to the culture media at a concentration of 5, 10, and 50 mM for three respective test groups while the control group was cultured without Sodium chlorate. Samples for different studies were collected at day 1, 7, and 14. The groups have been coded as given in Table 1 that has been used throughout the manuscript.

Murab S., Chameettachal S, & Ghosh S. (2016). Establishment of an in vitro monolayer model of macular corneal dystrophy. Laboratory investigation; a journal of technical methods and pathology, 96(12).

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