Laquatwin ph 11

Soil samples (0–20 cm) were taken on 25 December 2018 and then air-dried for the determination of soil properties: EC, pH, total N content, alkali hydrolysable N content, Olsen P content, available K content, organic C content and aggregate size distribution. Soil pH was determined at soil:water ratio (w/w) of 1:2.5 using a pH meter (model LAQUAtwin pH-11, Horiba, Kyoto, Japan). Soil EC was determined at soil:water ratio (w/w) of 1:5 using conductivity meter (model MP515 Shanghai Sanxin, Shanghai, China). Soil total N content was examined with a continuous flow analyzer (Auto Analyzer 3 System, SEAL Analytical GmbH, Norderstedt, Germany), while available K content was analyzed with an atomic absorption spectrophotometer (ZEEnit700P, Analytic Jena, Jena, Germany). Detailed information can be found in Sun et al. [26 (link)]. Alkali hydrolysable N content was determined according to Lu [27 ] and Olsen P content was determined according to Olsen et al. [28 ]. Soil organic C content was determined following Nelson and Sommers [29 ]. Soil aggregate size distribution was determined with wet-sieving method [30 ].

The pH of the aqueous solution of α-T3DMG with or without TCA is described in Section 4.7.1. It was measured using a LAQUAtwin pH-11 pocket water quality meter (HORIBA, Ltd., Kyoto, Japan).

6–8-wk-old mice were sacrificed, and the intestine was isolated. Approximately 2-cm pieces of ileum and colon were cut open longitudinally, and the luminal side was exposed using a pair of forceps. The luminal pH and sodium ion concentration were measured using a pH meter (LAQUAtwin pH11; Horiba) and sodium ion meter (LAQUAtwin Na-11; Horiba), respectively.
To determine fecal sodium ion concentrations, fresh feces were collected from mice in preweighed tubes and weighed and subjected to lyophilization. Double-distilled water was added to each sample (100 mg/ml), and the fecal pellets were homogenized using a Spinwin micro pestle (Tarsons). The samples were vortexed for 30 s, followed by centrifugation at 3,000 rcf for 5 min. Sodium ion in the supernatant was measured using a sodium ion meter (LAQUAtwin Na-11; Horiba) and normalized to the weight of the dry feces.