N cadherin polyclonal antibody 22018 1 ap

Antibodies used in this study included: GAPDH monoclonal antibody (ab125247), snail monoclonal antibody (ab53519) and E-cadherin polyclonal antibody (ab1416) were purchased from ABCAM (UK); LAMC2 monoclonal antibody (YT5379) was purchased from Immunoway (USA); integrin β1 monoclonal antibody (sc-9970) was purchased from Santa Cruz (USA), p-FAK-Y397 (AP0302), FAK monoclonal antibody (A11131), fibronectin polyclonal antibody(A16678) and SATB1 polyclonal antibody (A5800) were purchased from ABclonal (China); N-cadherin polyclonal antibody (22018-1-AP) was purchased from Proteintech (USA). Secondary antibodies (ab288151 and ab150113) were purchased from ABCAM(UK). Polyvinylidene difluoride membranes with a pore size of 0.45 mm was purchased from Millipore (USA). RNAEX reagent, Evo M-MLV RT Premix and SYBR Green Premix Pro Taq HS qPCR Kit were purchased from Accurate Biotechnology (China), an RT-PCR system (TaKaRa, Japan). IL-6, IL-1β and TNF-α ELISA kits (Enzyme-linked Biotechnology Co., LTD, Shanghai, China) were used. Duolink PLA kits (DUO92012), Duolink PLA Rabbit PLUS (DUO92002), and PLA Mouse MINUS (DUO92004) proximity probes were purchased from Sigma-Aldrich.

The PD-L1 mouse monoclonal antibody (66248-1-Ig), E-cadherin polyclonal antibody (20874-1-AP), N-cadherin polyclonal antibody (22018-1-AP), and α-tubulin monoclonal antibody (66031-1-Ig) were obtained from Proteintech (Wuhan, Hubei, China). Vimentin rabbit monoclonal antibody (#5741), anti-mouse IgG antibody (ab190475), and anti-rabbit IgG antibody (ab205718) were purchased from Abcam (Shanghai, China).