Sn glycero 3 phosphocholine gpc

Anisic acid (ANISA) (1a) and veratric acid (VERA) (1b) were purchased from Sigma-Aldrich Chemical Co (St. Louis, MO, USA). sn-Glycero-3-phosphocholine (GPC) in the enantiomerically pure form was purchased from Bachem and converted to the cadmium chloride complex (GPC × CdCl₂) using described earlier method [16 (link)]. Phospholipase A₂ (Lecitase 10L; 10,000 LEU/mL) was a gift from Novozymes (Bagsvaered, Denmark). N,N’-Dicyclohexylcarbodiimide (DCC), 4-(N,N-dimethylamino)pyridine (DMAP), DBTO, TEA, palmitic acid, oxalyl chloride, dioctyl sulfosuccinate sodium salt (AOT), Dowex^® 50WX8 H⁺ form (an ion-exchange resin) were purchased from Sigma–Aldrich, as well as chloroform, methanol, and dichloromethane that were used in reactions and for column and thin-layer chromatography and isooctane for enzymatic hydrolysis. All of the solvents used in liquid chromatography were of HPLC grade (LiChrosolv Reagents) and were purchased from Merck (Kenilworth, NJ, USA).

sn-Glycero-3-phosphocholine (GPC) in the enantiomerically pure form was purchased from Bachem and converted to the cadmium chloride complex (GPC × CdCl₂) using the method described earlier [22 ]. Racemic acids: 3,7-dimethyl-3-vinyloct-6-enoic acid (1), (E)-3,7,11-trimethyl-3-vinyldodeca-6,10-dienoic acid (2), 2-(2-butylidene-1,3,3-trimethylcyclohexyl)-acetic acid (3) were obtained according to the procedure described before [23 ,24 ]. Organic solvents used in HPLC were purchased from Merck. Phospholipase A₂ (Lecitase 10 L; 10,000 LEU/mL) was a gift from Novozymes.