The sections used for IHC analysis of ID4, SLUG, E-cadherin, and Ki67 protein expression were first autoclaved in Antigen Retrieval Citra Solution (BioGenex, Milmont Dr, Fermont, CA, USA) at 121 °C for 10 min. The samples were then treated with 3% H2O2; and sequentially, subjected to incubation with 0.1% BSA for 1 hour, and then with a monoclonal anti-ID4 antibody (Santa Cruz Biotechnology, Dallas, TX, USA; 1:100), a polyclonal anti-SLUG antibody (Santa Cruz Biotechnology; 1:100), a monoclonal anti- E-cadherin (BD; 1:100), and a rabbit polyclonal anti-Ki67 (Abcam, Cambridge, UK; 1:500) overnight at 4 °C. Detection of the immunostaining was performed using the Super Sensitive Non-Biotin Polymer HRP Detection System (BioGenex), according to the manufacturer’s instructions.
E cadherin
Manufactured by BioGenex
Sourced in United States
E-cadherin is a cell adhesion protein that plays a crucial role in maintaining cell-cell adhesion and tissue integrity. It is a calcium-dependent transmembrane glycoprotein that mediates homophilic cell-cell interactions, contributing to the formation and stability of adherens junctions between epithelial cells.
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2 protocols using e cadherin
1
Immunohistochemical Analysis of ID4, SLUG, E-cadherin, and Ki67
2
Tumor Margin Characterization by IHC
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The hematoxylin and eosin sections of the tumor were studied and the infiltrative margin was marked under microscopy. The paraffin embedded biopsy blocks were retrieved from our pathology archives. The paraffin embedded sections were subjected to immunohistochemical staining (IHC) using E-cadherin (ready to use; BioGenex, Fremont, CA. REF: AM390-5M, LOT: AM3900517) and Vimentin (ready to use; BioGenex, Fremont, CA. REF: AM074-5M, LOT: AM0740616).
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