Celltrace carboxyfluorescein diacetate succinimidyl ester cfse

Manufactured by Thermo Fisher Scientific

CellTrace carboxyfluorescein diacetate succinimidyl ester (CFSE) is a fluorescent cell labeling dye used for tracking and analyzing cell division in various applications, such as flow cytometry, cell proliferation assays, and cell-based research. The dye passively diffuses into cells and becomes fluorescently active upon cleavage of the acetate groups, allowing for the monitoring of cell division by measuring the progressive halving of the fluorescent signal with each cell division.

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Lab products found in correlation

Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Thermo Fisher Scientific (1 mentions) Anti cd40, by Thermo Fisher Scientific (1 mentions) Dynabeads mouse cd43 untouched b cells, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igm f ab 2, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

CFSE (2779 citations) CellTrace CFSE (320 citations) Carboxyfluorescein diacetate succinimidyl ester (CFSE) (55 citations) Carboxyfluorescein diacetate succinimidyl ester (44 citations) CellTrace carboxyfluorescein succinimidyl ester (3 citations)

2 protocols using celltrace carboxyfluorescein diacetate succinimidyl ester cfse

Suppression of T Cell Proliferation by Tregs

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In this assay, CD62L⁺CD44^–CD4⁺ T cells labeled with CellTrace carboxyfluorescein diacetate succinimidyl ester (CFSE; Life Technologies) served as responder T cells. Antigen-presenting cells were prepared by depleting T cells from WT B6 splenocytes using Thy1-specific MACS beads. Responder cells (5 × 10⁴) were cultured with irradiated antigen-presenting cells (2 × 10⁵) and anti-CD3 (2C11; 0.3 μg/mL) in the absence or presence of various numbers of Tregs for 72 h. The division of responder T cells was assessed by dilution of CellTrace CFSE, and the division index was calculated using FlowJo software.

He N., Fan W., Henriquez B., Yu R.T., Atkins A.R., Liddle C., Zheng Y., Downes M, & Evans R.M. (2017). Metabolic control of regulatory T cell (Treg) survival and function by Lkb1. Proceedings of the National Academy of Sciences of the United States of America, 114(47), 12542-12547.

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Murine B Cell Activation Assays

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Primary B cells were negatively isolated from 8–12 week old mice using Dynabeads® Mouse CD43 (Untouched B cells) (Life Technologies) per manufacturer’s instructions. Purified B cells were maintained at 10⁶/mL in RPMI (Dibco) supplemented with 10% Fetal Bovine Serum, 50μM β-mercaptoethanol, 100mM sodium pyruvate, 100mM HEPES buffer, and 1% penicillin/streptomycin. B cells were activated with goat F(ab)₂ anti-mouse IgM (10μg/ml; Jackson ImmunoResearch), anti-CD40 (1μg/ml; eBioscience), IL4 (10ng/ml), LPS (500ng/ml), BAFF (100ng/ml; Peprotech) as indicated.
For cell proliferation and immunoglobulin production assays, purified B cells were stained with Cell Trace Carboxyfluorescein diacetatesuccinimidyl ester (CFSE, 5μm; Life Technologies), and cultured at 10⁶ cells/ml for 5 days with indicated stimuli. After 5 days, cells were subjected to flow cytometry and analysis.

Kong S., Thiruppathi M., Qiu Q., Lin Z., Dong H., Chini E.N., Prabhakar B.S, & Fang. D. (2014). Deleted in Breast Cancer 1 (DBC1) is a Suppressor of B cell Activation by Negatively Regulating Alternative NF-κB Transcriptional Activity. Journal of immunology (Baltimore, Md. : 1950), 193(11), 5515-5524.

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