Brahms anti tpon radioimmunoassay ria kit

Thyroid assessment was done by free T4, TSH, and TPO-Ab. Serum TPO-Ab concentrations were determined using a BRAHMS anti-TPOn radioimmunoassay (RIA) kit (Thermo Scientific, Bonn, Germany) with a functional sensitivity of 30 U/mL. TPO-Ab levels exceeding 60 U/mL were considered positive for TPO-Ab. Levels of serum TSH and free T4 were measured using a TSH-CTK-3 immunoradiometric assay kit (DiaSorin S.p.A, Saluggia, Italy) and a FT4 RIA kit (Beckman Coulter, IMMUNOTECH, Prague, Czech Republic), respectively.
Our center uses the Immulite 2000 H. pylori IgG system (Siemens AG, Erlangen, Germany) to measure anti-H. pylori IgG [18 (link)]. This test consists of a solid-phase, 2-step, chemiluminescent enzyme immunoassay.

The serum 25(OH)D3 concentration was determined using the DIA source 25OH-Vit.D3-Ria-CT Kit (DIAsource ImmunoAssays S.A., Louvain-La-Neuve, Belgium; Cobra II Auto-γ Counting System, Packard Instruments, Downers Grove, IL, USA). We defined 25(OH)D3 levels as vitamin D deficient (< 10 ng/mL, n = 280 [5.4%]), insufficient (10 to 30 ng/mL, n = 3,285 [63.3%]), sufficient (30 to 50 ng/mL, n = 1,257 [24.3%]), or excess (> 50 ng/mL, n = 364 [7%]) [25 (link),27 (link)]. The serum TPO-Ab concentration was determined using a BRAHMS anti-TPOn Radioimmunoassay (RIA) kit (Thermo Scientific, Darmstadt, Germany) with a functional sensitivity of 30 U/mL. A TPO-Ab level exceeding 60 U/mL was considered to constitute a positive TPO-Ab reading. Levels of serum TSH and free T4 were measured using the TSH-CTK-3 immunoradiometric assay kit (DiaSorinS.p.A, Saluggia, Italy) and free T4 RIA kit (Beckman Coulter/Immunotech, Prague, Czech Republic), respectively.