Neo 600 mhz system

Manufactured by Bruker

The Bruker Neo 600 MHz system is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for advanced research and analytical applications. The core function of the system is to provide a powerful platform for the analysis and characterization of chemical and biological samples through the use of NMR spectroscopy techniques.

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Lab products found in correlation

G2 spirit tem, by Thermo Fisher Scientific (1 mentions) Lacey carbon support, by Electron Microscopy Sciences (1 mentions) Qci f cryoprobe, by Bruker (1 mentions) Syringe filter, by Merck Group (1 mentions) Hfcn cryoprobe, by Bruker (1 mentions)

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Neo 600 MHz (6 citations) NEO 600 (6 citations)

2 protocols using neo 600 mhz system

TEM and NMR Analysis of PA-Losartan Complex

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Samples for transmission electron microscopy (TEM) were resuspended at a PA concentration of 10 mg/mL and then diluted 10-fold–1 mg/mL immediately before 10 µL of sample solution was transferred to plasma-cleaned 300-mesh copper grids with lacey carbon support (Electron Microscopy Science). Samples were stained with 2% uranyl acetate. Imaging was performed using a FEI Spirit G2 TEM working at 120 kV accelerating voltage.
Nuclear magnetic resonance (NMR) spectroscopy was performed on a Bruker Neo 600 MHz system with QCI-F cryoprobe at 298 K. The lyophilized powders from the sample preparation protocol above were resuspended in D₂O at 10 mg/mL PA concentration and 5 mg/mL losartan concentration. This corresponds to 8.7 mM E-PA, 8.7 mM K-PA, and 10.8 mM losartan.

Yamaura K., Sather N.A., Metlushko A., Nishimura H., Pavlović R.Z., Hambright S., Ravuri S.K., Philippon M.J., Stupp S.I., Bahney C.S, & Huard J. (2023). Sustained-release losartan from peptide nanofibers promotes chondrogenesis. Frontiers in Bioengineering and Biotechnology, 11, 1122456.

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Quantifying Urea in Synthetic Sweat

Cited 1 time

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All ¹H NMR experiments were performed on a Bruker Neo 600 MHz system equipped with a z-gradient HFCN Cryo probe. A series of standard urea solutions ranging from 1 mM to 500 mM was prepared by dissolving 0.3003 g urea crystal in 5 mL artificial sweat and diluting with artificial sweat. Samples of sweat were passed through syringe filters with pore size 0.2 μm (Sigma-Aldrich, MO, USA). Each sample was mixed with 5% D₂O (v/v) and 500 μL volumes of each were loaded into a standard 5 mm NMR tube for the measurements. Urea and water signals were measured following a reported sequence.^{49 (link)} The receiver gain was fixed at 25.4 for all sample. For different samples, the free induction decay (FID) signal was set from 1–64 for a decent signal to noise ratio. Each sample was analyzed 3 times.

Zhang Y., Guo H., Kim S.B., Wu Y., Ostojich D., Park S.H., Wang X., Weng Z., Li R., Bandodkar A.J., Sekine Y., Choi J., Xu S., Quaggin S., Ghaffari R, & Rogers J.A. (2019). Passive sweat collection and colorimetric analysis of biomarkers relevant to kidney disorders using a soft microfluidic system. Lab on a chip, 19(9), 1545-1555.

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