Ptarget pt vector

Manufactured by Promega

Sourced in Germany

The PTarget (pT) vector is a high-copy-number, bacterial expression vector used for the cloning and expression of recombinant proteins in E. coli. The vector features a T7 promoter for high-level, inducible protein expression, a multi-cloning site for insertion of the gene of interest, and an antibiotic resistance marker for selection of transformed cells.

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Lab products found in correlation

On targetplus smartpool, by Thermo Fisher Scientific (1 mentions) Gotaq green master mix, by Promega (1 mentions)

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PTarget (26 citations) PTarget vector (17 citations)

3 protocols using ptarget pt vector

Transient Transfection Assay for Characterizing PPARγ and MTMR7

Cited 1 time

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Chemicals were from Merck/Sigma (Darmstadt, Germany). Antibodies (Abs) used are listed in Table. S2. Untagged and GFP-tagged PPARγ1 and PPRE-luc plasmids were mentioned previously^{15 (link)}. Human full-length (FL) MTMR7 cDNA (start codon MEHIRT, aa 1–660, 76 kDa, NM_004686.4) was in pTarget (pT) vector (Promega GmbH, Mannheim, Germany) with or without GFP-tag^{25 (link)}. Transient transfection and luciferase assays were performed as described^{63 (link)}.

Weidner P., Söhn M., Schroeder T., Helm L., Hauber V., Gutting T., Betge J., Röcken C., Rohrbacher F.N., Pattabiraman V.R., Bode J.W., Seger R., Saar D., Nunes-Alves A., Wade R.C., Ebert M.P, & Burgermeister E. (2020). Myotubularin-related protein 7 activates peroxisome proliferator-activated receptor-gamma. Oncogenesis, 9(6), 59.

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MTMR7 cDNA Cloning and Expression

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The human full-length (FL) cDNA of MTMR7 (start codon MEHIRT, aa 1-660, 76 kDa, NM_004686.4) was amplified from total RNA extracted from HEK293T cells and inserted with and without an N-terminal FLAG-tag into pTarget (pT) vector (Promega GmbH, Mannheim, Germany). Empty vector (EV) pT was used as a negative control.

Weidner P., Söhn M., Gutting T., Friedrich T., Gaiser T., Magdeburg J., Kienle P., Ruh H., Hopf C., Behrens H.M., Röcken C., Hanoch T., Seger R., Ebert M.P, & Burgermeister E. (2016). Myotubularin-related protein 7 inhibits insulin signaling in colorectal cancer. Oncotarget, 7(31), 50490-50506.

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Cloning and Luciferase Assays of TUSC3

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Full length (FL) TUSC3 (NM_006765) cDNA was PCR-amplified from total RNA of SW480 cells using GoTaq Green Mastermix (Promega GmbH, Neckarau, Germany) and inserted with and without N-terminal FLAG tag in pTARGET (pT) vector (Promega). Luciferase reporter plasmids HRE-luc, PPRE-luc, SRE-luc and TOPFLASH-luc were described elsewhere [41 (link)]. TUSC3-siRNA and control-siRNA oligonucleotides were from Dharmacon (SMARTpool: ON-TARGETplus, Thermo Scientific, Lafayette, CO).

Burgermeister E., Höde P., Betge J., Gutting T., Merkel A., Wu W., Tänzer M., Mossner M., Nowak D., Magdeburg J., Rückert F., Sticht C., Breitkopf-Heinlein K., Schulte N., Härtel N., Belle S., Post S., Gaiser T., Heppner B.I., Behrens H.M., Röcken C, & Ebert M.P. (2017). Epigenetic silencing of tumor suppressor candidate 3 confers adverse prognosis in early colorectal cancer. Oncotarget, 8(49), 84714-84728.

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