Uas cdc42 rnai

Manufactured by Bloomington Drosophila Stock Center

Sourced in United States

The UAS-cdc42-RNAi- is a genetic tool used for targeted gene silencing in Drosophila. It contains a UAS promoter that drives the expression of an RNAi construct targeting the cdc42 gene, which is involved in various cellular processes. This product allows researchers to investigate the functional role of the cdc42 gene in Drosophila models.

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2 protocols using uas cdc42 rnai

Genetic Tools for Drosophila Cytoskeleton Studies

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W1118, GMR-Gal4(9146), long-GMR-Gal4(8121), UAS-rac1W(28874), rac1^J11 (6674), rac1^J11, rac2^Δ(6677), rac1^J10, rac2^Δ,, mtl^Δ,(6679), cdc42¹(7337), Rho1^E3.10(3176), UAS-rac1-RNAi(34910), UAS-YFP-rab21(23241), UAS-rab21-RNAi(29403), UAS-rab7-GFP(42706), UAS-rab7-RNAi(27051), vav^KG02022(14176), UAS-cdc42-RNAi-1#(29004), UAS-cdc42-RNAi-2#(37477), UAS-Rho1-RNAi-1#(9909), UAS-Rho1-RNAi-2#(29002), UAS-trio-RNAi-3#(27732), UAS-trio-RNAI-4#(43549), UAS-sos-RNAi-3#(31275), UAS-sos-RNAi-4#(34833), and UAS-vav-RNAi(39059) lines were provided by Bloomington Drosophila Stock Center, Bloomington, IN, USA. UAS-rac1-RNAi (v49246), UAS-egr-RNAI-1#(v45252), UAS-egr-RNAi-2#(v45253), UAS-trio-RNAi-1#(v40137), UAS-trio-RNAi-2#(v40138), UAS-sos-RNAi-1#(v42848), UAS-sos-RNAi-2#(v106925), UAS-dLRRK-RNAi-1#(v22139), and UAS-dLRRK-RNAi-2#(v22140) were provided by Vienna Drosophila RNAi Center, Vienna, Austria. UAS-egr was provided by Dr. Konrad Basler.^{6 (link)} sqh-PAK1^RBD-GFP was provided by Dr. Susan Parkhurst.^{22 (link)} TRE-GFP was provided by Dr. Dirk Bohmann.^{23 (link)} UAS-GFP-myc-2xFYVE was provided by Dr. Hugo Bellen (Baylor College of Medicine, Houston, TX, USA). UAS-dLRRK-WT, UAS-dLRRK-I1915T, and UAS-dLRRK-3KD were provided by Dr. Bingwei Lu.^{43 (link)}

Ruan W., Srinivasan A., Lin S., Kara K.I, & Barker P.A. (2016). Eiger-induced cell death relies on Rac1-dependent endocytosis. Cell Death & Disease, 7(4), e2181-.

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Drosophila Genetic Toolkit for Rab5 Study

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Rab5^Z1 was an EMS allele generated in our lab; Rab5² was from Marcos González; UAS-Rab5 GFP/TM3, UAS-Rab5 RNAi/CyO, and UAS-Rab5 RNAi/TM3 were from Xinhua Lin; C587-GAL4 was from Ting Xie; nosGAL4VP16 was from Ruth Lehmann; puc^E69 (puc-lacZ) was from Joaquim Culi; kek1-lacZ is an enhancer trap line (BB142) from T. Schüpbach; UAS-YFP-Rab5 (BL24616), UAS-TSG101-RNAi (BL35710), UAS-Vps25-RNAi (BL26286), UAS-avl-RNAi (BL29546), UAS-Cdc42-RNAi (BL28021), dpp^P10638 (dpp-lacZ), tj-GAL4, tub-GAL80^ts, UAS-dpp-RNAi (BL25782), UAS-bsk^DN, UAS-hep^CA, and 2L deficiency flies were from Bloomington Drosophila Stock Center.
Fly stocks were maintained under standard culture conditions and all flies were dissected 0–2 days after eclosion unless otherwise indicated. For GAL4/GAL80^ts controlled RNAi or gene expression, flies were raised at 18 or 25°, shifted to 29° upon eclosion, and aged at 29° for 5 days before dissection. Germline or somatic cyst cell specific clones were generated by expressing FLP with nosGAL4VP16 or C587GAL4 (Kawase et al. 2004 (link)), respectively.

Tang Y., Geng Q., Chen D., Zhao S., Liu X, & Wang Z. (2017). Germline Proliferation Is Regulated by Somatic Endocytic Genes via JNK and BMP Signaling in Drosophila. Genetics, 206(1), 189-197.

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