B4galt1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

B4GALT1 is a protein-encoding gene that produces an enzyme involved in the synthesis of various glycoconjugates. This enzyme catalyzes the transfer of galactose to N-acetylglucosamine, a key step in the formation of complex carbohydrates.

Automatically generated - may contain errors

Lab products found in correlation

Horseradish peroxidase, by GE Healthcare (1 mentions) Vinculin, by Proteintech (1 mentions) Pd l1, by Abcam (1 mentions) Gapdh, by Proteintech (1 mentions) Biomax film, by Kodak (1 mentions) Iblot, by Thermo Fisher Scientific (1 mentions) Socs3, by Santa Cruz Biotechnology (1 mentions)

2 protocols using b4galt1

Western Blot Antibody Optimization

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Western blotting was performed using standard methods. The following antibodies were used: RBMS1 (Abcam ab150353), PD-L1(CST 13684), PD-L1 (Abcam ab213480), B4GALT1 (Santa Cruz sc-515551), VINCULIN (Proteintech 66305-1-Ig), GAPDH (Proteintech 60004-1-Ig), TUBULIN (ABclonal AC006). Secondary antibodies were conjugated to horseradish peroxidase (GE Healthcare). The protein bands were probed with an ECL Enhanced chemiluminescence regent Kit (NCM Biotech), visualized using the MiniChemi™ 500 image system (Beijing Sage Creation, China) and analyzed by Image software (Lane 1D, Beijing Sage Creation, China).

Zhang J., Zhang G., Zhang W., Bai L., Wang L., Li T., Yan L., Xu Y., Chen D., Gao W., Gao C., Chen C., Ren M., Jiao Y., Qin H., Sun Y., Zhi L., Qi Y., Zhao J., Liu Q., Liu H, & Wang Y. (2022). Loss of RBMS1 promotes anti-tumor immunity through enabling PD-L1 checkpoint blockade in triple-negative breast cancer. Cell Death and Differentiation, 29(11), 2247-2261.

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Western Blot Analysis of Cellular Proteins

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Cells were lysed directly in lysis buffer to collect whole cell extracts. Protein samples were separated on polyacrylamide gels, transferred onto nitrocellulose membrane by iblot (Invitrogen), detected using horseradish peroxidase-conjugated secondary antibodies, and exposed to BioMax film (Kodak) following chemiluminescence (Santa Cruz, CA, USA). The following primary antibodies were used: SOCS3, Actin, and B4GALT1 (Santa Cruz, CA, USA).

Liu Y.X., Wang L., Liu W.J., Zhang H.T., Xue J.H., Zhang Z.W, & Gao C.J. (2016). MiR-124-3p/B4GALT1 axis plays an important role in SOCS3-regulated growth and chemo-sensitivity of CML. Journal of Hematology & Oncology, 9, 69.

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