Alpha-methoxy-ω-amino PEG (MeO-PEG-NH2; Mn = 2400; Mw/Mn = 1.11; NOF, Tokyo, Japan) was purified using an ion-exchange CM Sephadex C-50 column (GE Healthcare, Buckinghamshire, UK) before use. Alpha-acetal-ω-amino PEG (acetal-PEG-NH2) was synthesized as described previously [27 (link)]. Beta-benzyl-l -aspartate N-carboxy-anhydride (BLA-NCA; NOF), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC; Tokyo Chemical Industry, Tokyo, Japan), Cyclo[RGDfk(CX-)] (cRGD peptide, X = 6-aminocaproic acid, ∊-Acp; Peptide Institute, Osaka, Japan), sulfo-Cy3 mono-reactive dye (Lumiprobe, Orlando, Florida, USA), sulfo-Cy5 mono-reactive dye (Lumiprobe), DyLight488 NHS ester (Thermo Fischer Scientific, Waltham, Massachusetts, USA), methylamine solution (Me–NH2; 40%; Wako Pure Chemical Industries, Osaka, Japan), sodium hydroxide (NaOH; Koso Chemical, Tokyo, Japan), hydrochloric acid (HCl; Koso Chemical), acetic acid (CH3COOH; Nacalai Tesque, Tokyo, Japan), ferucarbotran (SPIO) solution (Resovist®, Fujifilm RI Pharma, Tokyo, Japan), Blocking one (Nacalai Tesque), 4% paraformaldehyde (4% PFA; Wako), Hoechst 33342 (Hoechst; Dojindo Laboratories, Kumamoto, Japan), and other reagents were used without further purification. PBS-Tween solution (0.1% v/v PBS-T) was prepared from Dulbecco’s phosphate-buffered saline (D-PBS; Wako) and Tween 20 solution (10% w/v, Wako).
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc
Manufactured by Tokyo Chemical Industry
Sourced in Japan
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) is a chemical compound commonly used as a coupling agent in organic synthesis. It facilitates the formation of amide bonds between carboxylic acids and primary amines.
Automatically generated - may contain errors
Lab products found in correlation
N hydroxysuccinimide nhs, by Tokyo Chemical Industry (2 mentions)
Chitosan, by Merck Group (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Dylight 488 nhs ester, by Thermo Fisher Scientific (1 mentions)
D pbs, by Fujifilm (1 mentions)
Paraformaldehyde (pfa), by Fujifilm (1 mentions)
Blocking one, by Nacalai Tesque (1 mentions)
Biotin, by Merck Group (1 mentions)
Hydrogen peroxide h2o2, by Thermo Fisher Scientific (1 mentions)
Streptavidin coated polystyrene beads, by Spherotech (1 mentions)
Acetic acid, by Junsei (1 mentions)
Isobutyl chloroformate, by Tokyo Chemical Industry (1 mentions)
Xylazine hydrochloride, by Bayer (1 mentions)
Ketamine hydrochloride, by Daiichi Sankyo (1 mentions)
Silica gel, by Kanto Chemical (1 mentions)
Alginate, by Merck Group (1 mentions)
1 hydroxybenzotriazole hydrate, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Sodium cyanoborohydride nabh3cn, by Merck Group (1 mentions)
1 4 diaminobutane dab, by Merck Group (1 mentions)
9 protocols using 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc
1
Synthesis and Modification of PEG Polymers
2
Chitosan-HCA Conjugation for Biomedical Applications
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Chitosan (viscosity, 100 mPa·s; 70% deacetylated) was purchased from Heppe Medical Chitosan GmbH [Halle (Saale), Germany]. HCA was purchased from Sigma-Aldrich (Milwaukee, WI, USA). 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) was purchased from Tokyo Chemical Industry Co., Ltd. (TCI) (Tokyo, Japan). All other chemicals were of analytical grade.
3
Biotin-Antibody Surface Functionalization
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98% sulfuric acid (H2SO4) and 30% hydrogen peroxide (H2O2) were purchased from Fisher Scientific. (3-Aminopropyl)triethoxysilane (APTES), 4-ethoxymethylene-2 phenyl-2-oxazoline-5-one (oxazolone) and biotin were obtained from Sigma-Aldrich. (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (EDC) and N-Hydroxysuccinimide (NHS) were purchased from Tokyo Chemical Industry (TCI) Co., Ltd and Acros Organics, respectively. Streptavidin coated polystyrene beads (1.09 μm in diameter) were purchased from Spherotech Inc. biotinylated LYVE-1 monoclonal antibody (biotin-AntiLyve1) and biotinylated mouse IgG Isotype control (biotin-Isotype) were purchased from eBioscience. Phosphate buffered saline (1X, pH 7.4) was obtained from Thermo Scientific Inc.
4
Synthesis and Characterization of Brinzolamide
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4-Dimethylaminopyridine (DMAP), trimethylamine (NEt3), deuterochloroform (CDCl3), and sodium chloride (NaCl) were purchased from Wako Pure Chemical Industries, Ltd. Isobutyl chloroformate, pyridinium chlorochromate (PCC), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) were purchased from Tokyo Chemical Industry Co., Ltd. Tetrahydrofuran (THF), hydrochloric acid (HCl), ammonium chloride (NH4Cl), potassium carbonate (K2CO3) and acetic acid (AcOH) were purchased from Junsei Chemical Co., Ltd. t-Butyldimethylsilyl chloride, iodomethane (CH3I), potassium permanganate (KMnO4), dichloromethane (CH2Cl2), hexane, ethyl acetate (AcOEt), methanol (MeOH), acetonitrile (CH3CN), sodium bicarbonate (NaHCO3), anhydrous magnesium sulphate (MgSO4), and silica gel were purchased from Kanto Chemical Co., Ltd. Acetone was purchased from Daishin Chemical Co., Ltd. Brinzolamide was purchased from Yitai Pharmatec Co., Ltd. Isoflurane was purchased from Pfizer Co., Ltd. as Escain. Sevoflurane was purchased from Maruishi Pharmaceutical Co., Ltd. as Sevofrane. Ketamine hydrochloride was purchased from Daiichi-Sankyo Co., Ltd. as KETALAR. Xylazine hydrochloride was purchased from Bayer HealthCare Co., Ltd. as Selactar. Azopt was purchased from Alcon Co., Ltd. These reagents were used without further purification.
5
Chitosan-Gallic Acid Biopolymer Synthesis
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Chitosan (CHI, 75–85% deacetylated, 50,000–190,000 Da), gallic acid (GA), 1-hydroxybenzotriazole hydrate (HOBt), and alginate (ALG), were purchased from Sigma-Aldrich (St. Louis, MO, USA). Furthermore, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), whereas punicalagin (PC) was purchased from Santa Cruze Biotechnology (Santa Cruz, CA, USA).
6
Fluorescent Nanodiamonds for Biomedical Applications
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Fluorescent nanodiamonds (FNDs, carboxylated, 100 nm, 1 mg mL−1) in DI water) were obtained from Adamas Nano (Raleigh, NC), sodium hyaluronate (HA, 200 kDa) from Lifecore Co. (Chaska, MN) and N-hydroxysulfosuccinimide (sulfo-NHS) from Georgiachem. 1,4-Diaminobutane (DAB) and sodium cyanoborohydride (NaBH3CN) were purchased from Sigma Aldrich (Seoul, Korea). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 5-aminofluorescein (FTIC) were purchased from Tokyo Chemical Industry (Tokyo, Japan). Dulbecco's modified Eagle's medium-high glucose (DMEM), fetal bovine serum (FBS), antibiotics, and phosphate buffered saline (PBS, pH 7.4) were obtained from Invitrogen (Carlsbad, CA). Cell counting kit-8 (CCK-8) was obtained from DoGenBio Co. Human liver epithelial cell line (FL83B), human liver cancer cell line (HepG2), adenocarcinomic human alveolar basal epithelial cells (A549) and human embryonic kidney 293 cells (HEK293) were purchased from Korean Cell Line Bank (Seoul, Korea). 6 week-old female balb/c nude mice were purchased at the Orient Bio (Seongnam, Korea). Alfalfa free feed (AIN-93G) for mouse was purchased from Saeronbio Co. (Uiwang, Korea). All animal experiments followed the guidelines for Care and Use of Laboratory Animals of the Pohang University of Science and Technology (POSTECH) and approved by the Institutional Animal Care and Use Committee (IACUC).
7
Synthesis and Characterization of Gold Nanoparticles
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Gelatin type B, 2-morpholinoethanesulfonic acid (MES), sodium chloride, gold (III) chloride hydrate, sodium citrate, NAC and Ty were purchased from Sigma-Aldrich (St Louis, MO, USA). N-Hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) were purchased from Tokyo Chemical Industry (Tokyo, Japan). Ty, HRP, and Alexa Fluor 488 Phalloidin were purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). Spectra/Por 4 dialysis tubing (3.5 and 10 kDa MWCO) was purchased from Spectrum Laboratories Inc (Rancho Dominguez, CA, USA). DMEM, FBS, antibiotic agents (penicillin/streptomycin, PS), TrypLE™ Express, PBS, and Dubecco’s phosphate buffered saline (DPBS) were purchased from Gibco (Thermo Fisher Scientific). Deionized-distilled water (DW) was produced by an ultrapure water system (Puris-Ro800; Bio Lab Tech., Republic of Korea).
8
Chitosan-Hydrocaffeic Acid Composite Cement
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Chitosan (medium molecular weight; degree of deacetylation: 75–85%) and hydrocaffeic acid (HCA; 3-(3,4-dihydroxyphenyl)propionic acid) were purchased from Sigma-Aldrich. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and ethanol were purchased from Tokyo Chemical Industry (Tokyo, Japan) and Fisher Scientific (Waltham, MA, USA), respectively. All the chemicals were of analytical grade and were used without further purification. A membrane with a molecular weight cut-off of 12–14 kDa (Spectra/Por, Spectrum Laboratories, Irving, TX, USA) was used for dialysis. Ultrapure water (Millipore, Burlington, MA, USA) was used in all experiments.
For the mortar, ordinary Portland cement (specific gravity: 3.15 g/cm3; Blaine: 3430 cm2/g; Asia company, Seoul, Korea) and fine aggregates (sand from the Namwon region; specific gravity: 2.6 g/cm3, Fineness modulus: 2.89) were used.
For the mortar, ordinary Portland cement (specific gravity: 3.15 g/cm3; Blaine: 3430 cm2/g; Asia company, Seoul, Korea) and fine aggregates (sand from the Namwon region; specific gravity: 2.6 g/cm3, Fineness modulus: 2.89) were used.
9
Synthesis and Characterization of MPA-CUR Conjugate
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MPA (MW 320.3 g/mol) was obtained from AK Scientific (Union City, CA, USA), and CUR (MW 368.4 g/mol) was synthesized and characterized by the previously published method [32 (link)]. The 1-Ethyl-3-(3-dimethylamino propyl) carbodiimide hydrochloride (EDC) was obtained from Tokyo Chemical Industry (Tokyo, Japan). Moreover, 4-Dimethylaminopyridine (DMAP) was bought from Sigma Aldrich (St. Louis, MO, USA). HaCaT cell line was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Caco-2 cell line (ATCC No. HTB37) was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma Aldrich (St. Louis, MO, USA). Other materials, including solvents and chemicals, were purchased from commercial sources and used without further purification.
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