Levels of ROS were measured using a dichlorodihydrofluorescein diacetate (DCFH-DA) detection kit (Sigma, St. Louis, MO, USA) according to the manufacturer’s instructions. The MCF-7 and MCF-10A cells were seeded in 96-well plates at a density of 5 × 103. After treatment, the medium was removed and the cells were incubated with 10 µM of DCFH-DA plus 100 µL of Hank’s buffered salt solution (HBSS) for 30 min at 37 °C. The levels of ROS were measured using a spectrofluorometer (LS50B, Waltham, MA, USA; Ex: 490 nm, Em: 570 nm).
Dcfh da detection kit
Manufactured by Merck Group
Sourced in United States
The DCFH-DA (2',7'-Dichlorodihydrofluorescein diacetate) detection kit is a laboratory product used for the detection and quantification of reactive oxygen species (ROS) in various biological samples. The kit provides a simple, sensitive, and reliable method to measure intracellular ROS levels. The DCFH-DA molecule is cell-permeable and is converted to a fluorescent product upon oxidation, allowing for the direct measurement of ROS.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dcfh da detection kit
1
Quantifying Cellular ROS Levels
2
Quantifying Intracellular ROS Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Levels of intracellular ROS were measured using the dichloro dihydro fluorescein diacetate (DCFH-DA) detection kit (Sigma, St. Louis, MO, USA, catalog number: MAK145) according to the manufacturer’s protocol. MCF-7 cells were seeded in 96-well plates at a density of 5×103 and treated with SIM, SIM-CB or either of the two compounds plus vitamin E for 48 hours. After treatment, medium was removed and cells were incubated with 10 µM of DCFH-DA and 100 µL Hank’s buffered salt solution (HBSS, Lonza, E.U.) for 30 min at 37°C. ROS levels were measured using a microplate reader (BioTek Synergy, Ex: 490 nm, Em: 570 nm).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!