Ammonium hydroxide solution (25% NH3 in H2O), paraffin wax, 3-aminopropyl triethoxysilane (APTES), ε-caprolactone, triethylamine (TEA), Tin(II) 2-ethylhexanoate Sn(Oct)2, acryloyl chloride(AC), DL-malic acid, cisplatin, Candida Antarctica Lipase B (CALB, with 3.14 U/L lipase activity, (Supplementary Section S1.1 )), sodium bis(2-ethylhexyl) sulfosuccinate (AOT), fluorescein, acridine orange, and dimethylformamide (DMF) were purchased from Merck Chemical Company. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-Hydroxysuccinimide (NHS) 98%, iron (II) chloride tetrahydrate (FeCl2(4H2O)) and iron (III) chloride hexahydrate(FeCl3(6H2O)) were obtained from Sigma-Aldrich. Chitosan oligosaccharide ( = 3000 g/mol) was purchased from Golden-Shell Pharmaceutical Company (Ltd.), China. Ethanol, chloroform, and phosphate-buffered saline (PBS, pH 7.4) were supplied from Kian Kaveh, Iran.
Dl malic acid
Manufactured by Merck Group
Sourced in United States, China, France
DL-malic acid is a chemical compound used as a laboratory reagent. It is a dicarboxylic acid that occurs naturally in various fruits and other organic materials. This product is primarily used for research and analytical purposes in chemistry, biochemistry, and related scientific fields.
Automatically generated - may contain errors
Lab products found in correlation
Citric acid, by Merck Group (7 mentions)
Glycine, by Merck Group (4 mentions)
D glucose, by Merck Group (4 mentions)
Citric acid monohydrate, by Merck Group (3 mentions)
Choline chloride, by Merck Group (3 mentions)
Gallic acid, by Merck Group (3 mentions)
Hydrochloric acid (hcl), by Merck Group (3 mentions)
Choline chloride, by Thermo Fisher Scientific (3 mentions)
Acetic acid, by Avantor (3 mentions)
Sodium hydroxide (naoh), by Merck Group (3 mentions)
L methionine, by Merck Group (2 mentions)
Iron 3 chloride hexahydrate, by Merck Group (2 mentions)
Cisplatin, by Merck Group (2 mentions)
L glutamic acid, by Merck Group (2 mentions)
Catechin, by Merck Group (2 mentions)
Succinic acid, by Merck Group (2 mentions)
Oxalic acid, by Merck Group (2 mentions)
Potato dextrose agar (pda), by Thermo Fisher Scientific (2 mentions)
L tartaric acid, by Merck Group (2 mentions)
L ascorbic acid, by Merck Group (2 mentions)
28 protocols using dl malic acid
1
Synthesis of Multifunctional Nanoparticles
2
Intracellular Metabolite Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sedoheptulose 7-phosphate and erythrose 4-phosphate were purchased from Carbosynth, cysteinylglycine from Bachem AG, and l -aspartic acid, citric acid monohydrate, l -glutamic acid, l -glutamine, glycine, l -leucine, dl -malic acid, l -methionine and l -valine from Merck. All other standard substances were obtained from Sigma Aldrich. A mix of 82 relevant intracellular metabolites was prepared from standard substances. Single standard solutions of each metabolite were obtained by exact weighing and dissolving the standard substance in a suitable solvent (0.1 M hydrochloric acid, 0.1 M sodium hydroxide or water). Equimolar mixtures of the 82 metabolites were used for initial method development. For the use as calibration standards, appropriate dilutions of equimolar stock mixes of the 82 metabolites were spiked with a defined volume of uniformly 13C-labeled yeast cell extract (prepared in-house42 (link)).
3
Analytical Standards and Shower Gel Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analytical standards of maleic acid, DL-malic acid, D-(−)-quinic acid, (+)-catechin, (−)-epicatechin, (−)-catechin 3-gallate, L-methionine, L-tryptophan, as well as potassium pyrosulfite (E224), were purchased from Merck (Darmstadt, Germany); gallic acid, quercetin, ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt and 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) from POL-AURA (Zabrze, Poland); tartaric acid from Chempur (,Piekary Slaskie, Poland); D-(+)-xylose and sucrose from SUPELCO (Pennsylvania, PA, USA), and DPPH (2,2-diphenyl-1-picrylhydrazyl from Sigma Aldrich (Saint Louis, MO, USA).
All standards used were of analytical grade (≥99% purity).
The shower gel formulations were made using certified, vegetable-based raw materials which are approved for the production of natural products according to ECOCERS and COSMOS standards: sodium coco-sulfate (Sulfopon 1216 G, BASF, Ludwigshafen, Germany), decyl glucoside (Plantacare 2000, BASF, Ludwigshafen, Germany), cocamidopropyl betaine (Rokamina K30, PCC-Excol, Brzeg Dolny, Poland), benzyl alcohol, benzoic acid, dehydroacetic acid, tocopherol as a preservative (Schülke & Mayr GmbH, Norderstedt, Germany), sodium chloride (POCH, Gliwice, Poland), citric acid (Krakchemia, Kraków, Poland), and distilled water.
All standards used were of analytical grade (≥99% purity).
The shower gel formulations were made using certified, vegetable-based raw materials which are approved for the production of natural products according to ECOCERS and COSMOS standards: sodium coco-sulfate (Sulfopon 1216 G, BASF, Ludwigshafen, Germany), decyl glucoside (Plantacare 2000, BASF, Ludwigshafen, Germany), cocamidopropyl betaine (Rokamina K30, PCC-Excol, Brzeg Dolny, Poland), benzyl alcohol, benzoic acid, dehydroacetic acid, tocopherol as a preservative (Schülke & Mayr GmbH, Norderstedt, Germany), sodium chloride (POCH, Gliwice, Poland), citric acid (Krakchemia, Kraków, Poland), and distilled water.
4
Stable Isotope Tracing of Metabolic Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antimycin A, Ascorbic acid, D-glucose, DL-malic acid, methylpyruvate, N, N, N′, N′-tetramethyl-p-phenylenediamine (TMPD), potassium dichloroacetate, pyruvic acid, rotenone, sodium L -lactate, succinic acid and UK-5099 were from Sigma-Aldrich. [U-13C]glucose tracer was from Cambridge Isotope Laboratories. 3H2O was from American Radiolabeled Chemicals and 14C tracers were from Perkin Elmer. AR-C155858 was purchased from Tocris Bioscience. 7ACC2 (7-aminocarboxycoumarin 2, see structure in Supplementary Note 1 ) and CPI-613 were synthesized and purified in our lab.
5
Extraction and Analysis of Bioactive Compounds from Glycyrrhiza Japonica
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole plants of GJ were purchased from Tongling Hetian Chinese Medicine Pieces Co. Ltd. (Anhui, China) and smashed into powder with a disintegrator (Tianjin Tester Instrument Co, Ltd., Tianjin, China). Analytical grade reagents including choline chloride (≥98% purity), betaine (≥98% purity), oxalic acid (≥98% purity), DL-malic acid (≥98% purity), citric acid (≥98% purity), and malonic acid (≥98% purity) were purchased from Sigma-Aldrich (Shanghai, China). Analytical pure methanol, ethanol, and acetone were purchased from Tianjin Tianli Chemical Reagent Co. Ltd. (Tianjin, China). EA (≥98% purity) was purchased from Sichuan Vicky Biotechnology Co., Ltd. (Sichuan, China). DPPH (≥98% purity) was purchased from Diyibio Biotechnology Co., Ltd. (Shanghai, China). The chromatographic grade methanol was purchased from Honeywell (Arizona, USA). The chromatographic grade phosphoric acid (≥98% purity) was purchased from Tianjin Kemiou Chemical Reagent Co., Ltd (Tianjin, China). Ultra-pure water produced by Milli-Q IQ7000 (Millipore Corp, USA) was used throughout the experiment.
6
RmsI Enzyme Co-Crystallization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The truncation mutant RmsI (G12-P258) was concentrated to 0.42 mM (13 mg/ml) in a solution containing 50 mM Tris-HCl pH8.5 and 80 mM NaCl. AdoMet (Sigma, USA, 180 mM stock solution) and RmsI were mixed at a molar ratio of 5:1 and incubated on ice for 6 h before performing co-crystallization experiments. The crystallization screen was performed by mixing 1μl RsmI-AdoMet mixture and 1μl well buffer in the 48-well XtalQuest crystallization plate (MiTeGen, USA). Crystals were grown at 291K using the sitting-drop vapor diffusion method. The final crystallization condition is 0.2 M DL-Malic acid (pH 7.0), 20% PEG3350 (Sigma, USA).
7
Longan Drying and Amino Acid Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The major material utilized in this study was dried whole longan, purchased from a local market at Lamphun, Thailand, which was dried by a hot air dryer at 80°C for 17 hr, then at 75°C for 20 hr, then at 65°C for 10 hr, and finally stored at 4°C until analysis.
Potato dextrose agar and bacteriological peptone were purchased from Oxoid.dl ‐Malic acid was purchased from Sigma‐Aldrich. Alanine, arginine, aspartic acid, gamma‐aminobutyric acid, glutamic acid, glycine, isoleucine, leucine, lysine, phenylAlanine, proline, serine, threonine, tyrosine, and valine were supplied by Sigma Chemicals. AccQ‐Fluor reagent kits containing borate buffer, reagent powder, and acetonitrile were supplied by Waters Co. Ltd.
Potato dextrose agar and bacteriological peptone were purchased from Oxoid.
8
Visualizing Cadmium Stress Response in Rice Roots
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rice roots were labeled with 10 μM 5-(and-6)-chlormethyl-20,70-dichlordi-hydrofluorescein diacetate, acetyl ester (CM-H2DCF-DA) (Invitrogen, Carlsbad, CA, United States) for 30 min, then treated with 25 μM CdCl2 in the presence or absence of 5 μM DL-malic acid (Sigma, St. Louis, MO, United States) for 3 h. Fluorescence images of rice root were taken using Canon EOS500D (Canon, Tokyo, Japan) attached to a fluorescent microscope (Leica, Wetzlar, Germany) equipped with green fluorescent filter (excitation 450–490 nm, emission 500–530 nm). Fluorescence intensity was then quantitated by ImageJ17 . All experiments were repeated at least three times. Statistical analyses of significant difference was carried out by Student’s t-test.
9
Comprehensive Antioxidant Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the reagents used were of analytical or HPLC grade. Methanol and ethanol (96%) were purchased from Fisher Chemicals (Fair Lawn, NJ, USA). DPPH (2,2-diphenyl-1-picrylhydrazyl), Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), acetic acid, sodium acetate, TPTZ (2,4,6-tris(2-pyridyl)-s-triazine), hydrochloric acid, iron (III) chloride hexahydrate, Folin-Ciocalteau reagent, sodium carbonate, gallic acid, MES monohydrate, tris(hydroxymethyl)aminomethane, L(+)-ascorbic acid, DL-malic acid, L(+) tartaric acid, β-carotene, vitamin B6, and vitamin E were purchased from Sigma-Aldrich (St. Louis, MO, USA). D(+)-Glucose and D(−)-Fructose were purchased from VWR International (Radnor, PA, USA). Lutein was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Celite was purchased from AppliChem (Darmstadt, Germany). Sodium hydroxide and Acetone were purchased from LabChem (Zelienople, PA, USA). Ultrapure water was obtained from a Synergy® Water Purification System (MilliporeSigma, Burlington, MA, USA).
10
Extraction and Characterization of Arctic Seaweed Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Arctic brown seaweed F. vesiculosus L. was collected from the littoral of the Barents Sea (Zelentskaya Bay, Murmansk region, Dalnie Zelentsy, Russia), identified by Dr. E. Obluchinskaya (voucher specimens 7.2021, E.D.O.) and deposited in the Collection of the Zoobentos Laboratory (Murmansk Marine Biology Institute, Murmansk, Russia). The seaweeds were carefully washed, cleaned from epiphytes, and dried at room temperature for 3 days to remove the surface moisture. Following its placement in a 50 °C vacuum oven for 1 to 2 days, the dried samples were pulverized using a Cyclotec mill (CT 293 Cyclotec, Foss, Hilleroed, Denmark) to pass through a screen with an aperture of 1.0 mm. Choline chloride was purchased from Acros Organics (Fair Lawn, NJ, USA), L -lactic acid and D(+)-glucose were from Panreac Química SLU (Barcelona, Spain), and DL-malic acid was from Sigma-Aldrich (St. Louis, MO, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!