Sigmaplot 9

Manufactured by Merck Group

Sourced in United States

SigmaPlot 9.0 is a data analysis and graphing software tool developed by Systat Software, Inc. It is designed to provide users with the ability to create and customize high-quality scientific and technical graphs and charts. The software offers a range of features, including the ability to import and analyze data from various sources, as well as the ability to create complex mathematical models and statistical analyses.

Automatically generated - may contain errors

Lab products found in correlation

Hrp conjugate clone 3f10, by Roche (1 mentions) Sigmastat 4, by Merck Group (1 mentions) Prism 5, by Merck Group (1 mentions) Prism 6, by Merck Group (1 mentions) Pc 10, by Narishige (1 mentions) Sigmastat 2, by Merck Group (1 mentions) Spss 19, by IBM (1 mentions) Sigmastat 3, by Merck Group (1 mentions) Prism 5, by GraphPad (1 mentions) Prism 6, by GraphPad (1 mentions)

Spelling variants of this product

SigmaPlot (794 citations) SigmaPlot ver. 9.0 (2 citations) SigmaPlot 9.0 graphic software for Windows (2 citations) Sigmaplot version 9.0 (2 citations)

33 protocols using sigmaplot 9

Circular Dichroism Spectroscopy of HucR Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD spectroscopy was performed on a Jasco J-815 circular dichroism spectrometer. HucR and mutant proteins (0.2 mg/ml) were diluted in CD buffer (50 mM sodium phosphate pH 7.0 or 50 mM acetate pH 5.0). Far-UV CD spectra were obtained using a quartz cuvette with 0.1 cm path length at room temperature for pH 7.0 and at 4 °C for pH 5.0. All measurements were collected in triplicate with 1 nm steps over the wavelength range from 250 to 180 nm. The secondary structure composition was predicted from the spectrum by the CDSSTR algorithm with protein reference set 7 from DichroWeb.^{36 (link), 37 (link)} The goodness of fit was determined from the NRMSD value, which was in the range of 0.001–0.016.
For thermal denaturation, ellipticity was monitored from 224 to 218 using a 0.1 cm quartz cuvette over the temperature range of 4–70 °C with 1 °C increments. The data were analyzed after correcting for buffer contribution to the signal using the four-parameter sigmoidal equation of Sigma Plot 9.

Deochand D.K., Perera I.C., Crochet R.B., Gilbert N.C., Newcomer M.E, & Grove A. (2016). Histidine switch controlling pH-dependent protein folding and DNA binding in a transcription factor at the core of synthetic network devices. Molecular bioSystems, 12(8), 2417-2426.

+ Open protocol

+ Expand

Protein Expression Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments were repeated at least three times and results were expressed as mean ± SE. One-way analysis of variance (ANOVA) was calculated with Sigma-Plot 9. A one-tailed t-test was used to compare any significant differences between control and treated groups. The criterion for statistical significance was p < 0.05. For western blotting data, band intensities were measured using ImageJ and normalized with GAPDH.

Narayan S., Jaiswal A.S., Sharma R., Nawab A., Duckworth L.V., Law B.K., Zajac-Kaye M., George T.J., Sharma J., Sharma A.K, & Hromas R.A. (2017). NSC30049 inhibits Chk1 pathway in 5-FU-resistant CRC bulk and stem cell populations. Oncotarget, 8(34), 57246-57264.

+ Open protocol

+ Expand

Statistical Analysis of Experimental Data

Check if the same lab product or an alternative is used in the 5 most similar protocols

Data were analyzed using SigmaPlot 9 via an unpaired Student’s t test. The collected parameters were expressed as mean, and P< 0.05 was considered significant, validating positive findings. Each experiment was repeated, and n indicated the number of experiments.

Mushtaq M., Mahmood M., Jabbar U, & Kim U.H. (2023). Essential role of CD38 in platelet aggregation through the PKC-mediated internalization and activation. BioImpacts : BI, 14(2), 27780.

+ Open protocol

+ Expand

Triplicates Significance Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

All the experiments were performed in triplicates. Sigma Plot-9 was used for data expression. P-values <0.05 were considered statistically significant.

Ansari S.A, & Damanhory A.A. (2023). Biotechnological application of Aspergillus oryzae β-galactosidase immobilized on glutaraldehyde modified zinc oxide nanoparticles. Heliyon, 9(2), e13089.

+ Open protocol

+ Expand

Kinetic Analysis of Enzyme Reaction

Check if the same lab product or an alternative is used in the 5 most similar protocols

The kinetic constants were determined
from a fit of the initial velocity data to eq 1 using SigmaPlot 9, where v is the initial velocity, E_t is the total enzyme concentration, k_cat is the turnover number, [A] is the substrate
concentration, and K_m is the Michaelis
constant.

Hobbs M.E., Williams H.J., Hillerich B., Almo S.C, & Raushel F.M. (2014). l-Galactose Metabolism in Bacteroides vulgatus from the Human Gut Microbiota. Biochemistry, 53(28), 4661-4670.

+ Open protocol

+ Expand

Plotting Experimental Data with SigmaPlot

Check if the same lab product or an alternative is used in the 5 most similar protocols

The data expressed in various studies was plotted using SigmaPlot-9 and expressed as (±) standard error. Each value represents the mean for three independent experiments performed in duplicate, with average standard deviations <5%.

Li H., Li S., Tian P., Wu Z, & Li Z. (2017). Prevention of Bacterial Contamination of a Silica Matrix Containing Entrapped β-Galactosidase through the Action of Covalently Bound Lysozymes. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(3), 377.

+ Open protocol

+ Expand

ELISA-binding assays for humanized VNAR proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rat, mouse, and HSAs used in ELISA-binding assays were from Sigma-Aldrich. For direct ELISA formats Nunc Maxisorp 96-well plates were coated at 1 µg/mL antigen in PBS and then blocked with 4% non-fat milk in PBS. Purified 6-his-tagged control and humanized VNAR proteins in PBS were diluted 1/3 into wells and double-diluted further across the plate. After incubation for 1 h, plates were washed three times with 0.05% Tween 20 in PBS. The detection of antigen bound VNARs was achieved by incubation with an anti-6-His HRP mAb for 1 h or where appropriate with an anti HA tag mAb HRP conjugate (clone 3F10; Roche) and developed by adding TMB substrate. When fully developed, the reactions were halted by the addition of 1 M H₂SO₄ absorbance measured at 450 nM. Data were processed using SigmaPlot 9.

Steven J., Müller M.R., Carvalho M.F., Ubah O.C., Kovaleva M., Donohoe G., Baddeley T., Cornock D., Saunders K., Porter A.J, & Barelle C.J. (2017). In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties to Facilitate Clinical Development. Frontiers in Immunology, 8, 1361.

+ Open protocol

+ Expand

Biological Assay Protocol for Hypothesis Testing

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments were repeated at least three times and results were expressed as mean ± SE. One-way analysis of variance (ANOVA) was calculated with Sigma-Plot 9. A one-tailed t-test was used to compare any significant differences between control and treated groups. The criterion for statistical significance was p < 0.05. For western blotting data, band intensities were measured using ImageJ and normalized with GAPDH.

Narayan S., Ramisetti S., Jaiswal A.S., Law B.K., Singh-Pillay A., Singh P., Amin S, & Sharma A.K. (2018). ASR352, A potent anticancer agent: Synthesis, preliminary SAR, and biological activities against colorectal cancer bulk, 5-fluorouracil/oxaliplatin resistant and stem cells. European Journal of Medicinal Chemistry, 161, 456-467.

+ Open protocol

+ Expand

Synergistic Effects of NSC49L and TRAIL Treatments

Check if the same lab product or an alternative is used in the 5 most similar protocols

Statistical analyses for synergies between NSC49L and TRAIL treatments and p21 status were performed using CalcuSyn software (http://www.biosoft.com/w/calcusyn.htm). The combination index (CI) was calculated by applying Chou-Talalay method and was used for synergy quantification (Chou, 2010 (link)). Student’s t test was used for comparisons in both in vitro and in vivo experiments. All experiments were repeated at least three times and results were expressed as mean ± SE. One-way analysis of variance (ANOVA) was calculated with Sigma-Plot 9. A one-tailed t-test was used to compare any significant differences between control and treated groups. The criterion for statistical significance was p < 0.05. For western blotting data, band intensities were measured using ImageJ and normalized with GAPDH. Graph Pad Prism version 9.1.0 was also used for data visualization and p value calculation.

Narayan S., Raza A., Mahmud I., Koo N., Garrett T.J., Law M.E., Law B.K, & Sharma A.K. (2022). Sensitization of FOLFOX-resistant colorectal cancer cells via the modulation of a novel pathway involving protein phosphatase 2A. iScience, 25(7), 104518.

+ Open protocol

+ Expand

Evaluating Neurological Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

Data are expressed as mean ± SEM. All statistical tests were done with SigmaPlot 9.0 software. Groups of three or more were analyzed with one-way ANOVA, followed by Tukey’s post hoc test. A value of P < 0.05 was considered statistically significant.

Bhuiyan S., McLendon P., James J., Osinska H., Gulick J., Bhandary B., Lorenz J.N, & Robbins J. (2016). In vivo definition of cardiac myosin-binding protein C’s critical interactions with myosin. Pflugers Archiv : European journal of physiology, 468(10), 1685-1695.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!