Anti dcnl1

Manufactured by Merck Group

Anti-DCNL1 is a laboratory reagent used for the detection and quantification of DCNL1 protein levels. It utilizes an antibody-based detection method to provide researchers with information about the expression of this protein in various biological samples.

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Lab products found in correlation

Anti acadvl pa5 29959, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 or 594, by Thermo Fisher Scientific (2 mentions) Anti actin, by Merck Group (1 mentions) Anti γh2ax, by Merck Group (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Anti flag m2, by Merck Group (1 mentions) Ab13970, by Abcam (1 mentions) Anti actin mab1501, by Merck Group (1 mentions) Anti γh2ax 05 636, by Merck Group (1 mentions) Anti flag m2 f3165, by Merck Group (1 mentions) Anti gapdh, by Merck Group (1 mentions)

2 protocols using anti dcnl1

Immunoblotting and Immunofluorescence Assays

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Immunoblotting: anti-FLAG M2 (F3165, Sigma, 1/2000); anti-DCNL1 (Clone 3D7, Sigma-Aldrich, 1/1000); anti-GAPDH (Cell Signalling Clone 14c10 cat. no. 2118, 1/2000); anti-Actin (MAB1501, Millipore, 1/1000); anti-cullin1 (718700, Life Technologies, 1/1000); anti-cullin2 (700179, Life Technologies, 1/5000); anti-ACADVL (PA5-29959, Thermo Scientific, 1/1000); mouse anti-GFP (ab184519, Abcam, 1/2000). Sheep polyclonals were raised against full-length KLHL3, the N-terminus of DCNL3, 4 and 5, Cul4A and 4B, and against the C-terminus of Cul3 and Cul5, and used at 1 µg/ml.
Immunofluorescence: mouse anti-FLAG M2 (F3165, Sigma, 1/1000); chicken anti-GFP (ab13970, Abcam, 1/1000); sheep anti-DCNL5 (our own 1/200); sheep anti-cullin4A (our own, 1/100); anti-γ-H2Ax (05-636, Millipore, 1/1000), and secondary antibodies conjugated to Alexa-Fluor-488 or -594 from Life Technologies (1/1000).

Keuss M.J., Thomas Y., Mcarthur R., Wood N.T., Knebel A, & Kurz T. (2016). Characterization of the mammalian family of DCN-type NEDD8 E3 ligases. Journal of Cell Science, 129(7), 1441-1454.

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Comprehensive Immunoblotting and Immunofluorescence Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblotting: anti-FLAG M2 (F3165) (1/2000), anti-DCNL1 Sigma-Aldrich(Clone 3D7) (1/1000), anti-GAPDH(1/2000), anti-Actin(MAB1501) Millipore (1/1000), anti-Cullin1(718700) (1/1000) and anti-Cullin2(700179) (1/5000) Life Technologies, anti-ACADVL (PA5-29959) ThermoScientific, mouse anti-GFP (ab184519) Abcam (1/2000). Sheep polyclonals were raised against full-length KLHL3, the N-terminus of DCNL3,-4,-5, Cul4A,-B and against the C-terminus of Cul3 and Cul5 and used at 1μg/ml.
Immunofluorescence: mouse anti-FLAG M2 (F3165)(1/1000) Sigma, chicken anti-GFP Abcam(ab13970) (1/1000), sheep anti-DCNL5(1/200), sheep anti-Cullin4A(1/100), anti-γ-H2Ax(05-636) (1/1000) from Millipore, secondary antibodies conjugated to Alexa Fluor 488 or 594 from Life Technologies (1/1000).

Keuss M.J., Thomas Y., Mcarthur R., Wood N.T., Knebel A, & Kurz T. (2016). Characterization of the mammalian family of DCN-type NEDD8 E3 ligases. Journal of Cell Science, 129(7), 1441-1454.

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