Unfractionated bovine bile salt

Manufactured by Merck Group

Unfractionated bovine bile salt is a laboratory reagent derived from the bile of cattle. It is a complex mixture of various bile salts and other compounds. The core function of unfractionated bovine bile salt is to serve as a surfactant and emulsifier in various scientific and research applications.

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Lab products found in correlation

Sodium glycodeoxycholate bile salt, by Merck Group (2 mentions) Taurocholic acid, by Merck Group (2 mentions) Benchmark plus microplate spectrophotometer, by Bio-Rad (2 mentions) Taurodeoxycholic acid, by Merck Group (2 mentions)

Spelling variants of this product

Bile salts (273 citations) Bovine bile (57 citations) Bovine bile salts (13 citations)

2 protocols using unfractionated bovine bile salt

Assessing Bifidobacterium Bile Tolerance

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To determine Bifidobacterium survival in bile, isolates were first grown in RCM and then subcultured using a 1:50 dilution into MRS ± 0.3% unfractionated bovine bile salt (Sigma-Aldrich), as described by [57 (link)]. After 48 h of stationary growth in an anaerobic chamber at 37 °C OD_600 nm using the Benchmark Plus microplate spectrophotometer (Bio-Rad) for both conditions. For the MRS plate the mean blank OD_600 nmvalue was 0.1585 and for the anaerobic plate it was 0.1825. Data shown are mean values from three experimental repeats.To assess bile salt hydrolyase activity, overnight cultures were spotted (3 mL) onto MRS plates supplemented with l-cysteine and 0.5% w/v of either taurocholic acid, taurodeoxycholic acid, and sodium glycodeoxycholate bile salt (Sigma-Aldrich). Bile salt precipitation was assessed after a maximum of 96 h of anaerobic incubation at 37 °C. For both assays, uninoculated MRS media were used as a control.

Lawson M.A., O’Neill I.J., Kujawska M., Gowrinadh Javvadi S., Wijeyesekera A., Flegg Z., Chalklen L, & Hall L.J. (2019). Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem. The ISME Journal, 14(2), 635-648.

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Evaluating Bifidobacterium Bile Tolerance

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To determine Bifidobacterium survival in bile, isolates were first grown in RCM and then subcultured using a 1:50 dilution into MRS ± 0.3% unfractionated bovine bile salt (Sigma-Aldrich), as described by [58 (link)]. After 48h of stationary growth in an anaerobic chamber at 37°C OD_600nm using the Benchmark Plus microplate spectrophotometer (Bio-Rad) for both conditions. For the MRS plate the mean blank OD_600nmvalue was 0.1585 and for the anaerobic plate it was 0.1825. Data shown is mean values from three experimental repeats. To assess bile salt hydrolyase activity, overnight cultures were spotted (3mL) onto MRS plates supplemented with L-cysteine and 0.5% w/v of either taurocholic acid, taurodeoxycholic acid, and sodium glycodeoxycholate bile salt (Sigma-Aldrich). Bile salt precipitation was assessed after a maximum of 96 hours of anaerobic incubation at 37°C. For both assays, uninoculated MRS media was used as a control.

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