R26 eyfp

R26^eYFP, R26^mT/mG and R26^Brainbow2.1 reporter mice and Rag^–/– mice were obtained from the Jackson Laboratory. The Ccl19^Cre9, Col6a1^Cre47, Ccl19^Tta12, Ltbr^fl/lf48, TnfR1^–/–49, Tnfr1^fl/fl50, Tnfr1^cneo23 strains were described previously. Fate-mapping experiments were performed via treating the pregnant dams with 1 mg doxycycline (Sigma Aldrich) intraperitoneally and maintenance of doxycycline in the drinking water (1 mg/ml). For the commensal microbiota studies, mice were housed in a single room and soiled cage bedding was routinely mixed twice per week and distributed among all cages from weaning (four weeks old) until the onset of the study (10-12 weeks old). All mice were bred and maintained on a C57BL/6 genetic background in the animal facilities of the Biomedical Sciences Research Center “Alexander Fleming” and the Kantonsspital St. Gallen under specific pathogen-free conditions.
Experiments were performed in accordance with all current European and national legislation and were approved by the Institutional Committee of Protocol Evaluation in conjunction with the Veterinary Service Management of the Hellenic Republic Prefecture of Attika and the Swiss federal and cantonal guidelines (Tierschutzgesetz) under the permissions SG01/18, SG12/17, SG11/19 and SG08/18 granted by the Veterinary Office of the Kanton of St. Gallen.

Mist1-CreERT2 mice (Shi et al., 2009 (link)), Cxcl12-dsRED mice (Ding and Morrison, 2013 (link)), Troy^−/− and Troy-BAC-CreERT2 mice (Fafilek et al., 2013 (link)), H/K-ATPase-Cxcl12 mice (Shibata et al., 2013 (link)), Eef1a1-LSL-Notch1(IC) mice (Buonamici et al., 2009 (link)), Wnt5a^flox mice (Miyoshi et al., 2012 (link)) were described previously. Cxcr4-EGFP mice were kindly provided by Richard J. Miller (Northwestern University Medical School, USA). LSL-Kras^G12D and LSL-Trp53^R172H mice were provided by Dr. Kenneth Olive (Columbia University, USA). Apc^flox mice were obtained from the National Cancer Institute (NCI). Lgr5-DTR-GFP mice were provided by Genentech. Cdh1^flox, R26-mTmG, R26-LacZ, R26-TdTomato, R26-Confetti, R26-EYFP, Cxcl12^flox, Tie2-Cre, Id2-GFP, and Cag-CreERT2 mice were purchased from the Jackson Laboratory. Cre recombinase was activated by oral administration of TAM (1–5mg/0.2mL corn oil, as indicated). All animal studies and procedures were approved by the ethics committees at Columbia University and the Academy of Sciences of the Czech Republic. Human stomach tissue sections were obtained from DGC patients who underwent surgical resection or endoscopic submucosal dissection from 2001 to 2012 at Gifu University Hospital, Gifu, Japan. All study protocols were approved by the ethics committees, and written informed consent was obtained from all patients.