Plasma IgA, IgG1, IgG2a, IgG2b and IgM levels were assessed with the use of Mouse Isotyping Panel 1 Assay kit from Mesoscale according to the company’s instructions (MSD Multi-spot Assays, Mesoscale Discovery, Rockville, Maryland). Plasma IgM antibodies targeting PC were detected with an ELISA kit from Athera Biotechnologies (Athera Biotechnologies AB, Solna, Sweden) according to the company’s instructions, with the exception of using peroxidase-conjugated anti-mouse IgM (Jackson ImmunoResearch, Novakemi, Handen, Sweden).
Peroxidase conjugated anti mouse igm
Manufactured by Jackson ImmunoResearch
Sourced in Sweden
Peroxidase-conjugated anti-mouse IgM is a laboratory reagent used to detect the presence of mouse immunoglobulin M (IgM) in samples. It consists of an anti-mouse IgM antibody conjugated to the enzyme peroxidase, which can be used in various immunoassay techniques.
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2 protocols using peroxidase conjugated anti mouse igm
1
Quantification of Murine Antibody Isotypes
2
Immunoglobulin Levels Quantification
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Plasma IgA, IgG1, IgG2a (not expressed in C57BL/6 mice), IgG2b, IgG3 and IgM levels were assessed with the use of Mouse Isotyping Panel 1 Assay kit from Mesoscale according to the company’s instructions (MSD Multi-spot Assays, Mesoscale Discovery, Maryland, USA). Plasma IgE levels were determined with the use of mouse IgE ELISA Kit from Bethyl Laboratories (Bethyl Laboratories Inc, Montgomery, Texas, USA) while IgM antibodies targeting phosphorylcholine were assessed with an ELISA kit from Athera Biotechnologies (Athera Biotechnologies AB, Solna, Sweden) according to the company’s instructions, with the exception of the use of peroxidase-conjugated anti mouse IgM (Jackson ImmunoResearch).
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