Docetaxel (DTX) was obtained from Phyton Biotech GmbH (Ahrensburg, Germany). N,N’-dicyclohexyl-carbodiimide (DCC), 4-dimethylaminopyridine (DMAP), 4-methoxyphenol, methacrylic anhydride, ammonium acetate, formic acid, Mukaiyama’s reagent (2-chloro-1-methylpyridinium iodide), potassium peroxymonosulfate (oxone), potassium persulfate (KPS), lactic acid, N, N, N’, N’ tetramethyl-ethylenediamine (TEMED) and trifluoroacetic acid (TFA) were obtained from Sigma Aldrich (Zwijndrecht, the Netherlands). Dichloromethane (DCM), N,N-dimethylformamide (DMF) and acetonitrile (ACN) were purchased from Biosolve (Valkenswaard, the Netherlands). Absolute ethanol and triethylamine were purchased from Merck (Darmstadt, Germany). The macro-initiator (mPEG5000)2-ABCPA and 2-(2-(methacryloyloxy)ethylthio) acetic acid (linker) were synthesized as described previously [10 , 30 ]. Sucrose and trehalose were purchased from Merck (Darmstadt, Germany). Three-ml sample glass vials (soda-lime glass) with silicon caps were purchased from VWR (Langenfeld, Germany). All materials were analytical grade and used without purification.
N n n n tetramethylethylenediamine temed
Manufactured by Merck Group
Sourced in United States, Germany, Poland
N,N,N′,N′-tetramethylethylenediamine (TEMED) is a chemical compound commonly used as a catalytic agent in various laboratory applications. It is a clear, colorless liquid with a distinctive amine-like odor. TEMED is primarily used to accelerate the polymerization process in gel electrophoresis techniques, such as SDS-PAGE and native PAGE, where it helps to initiate and maintain the polymerization of acrylamide monomers.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium persulfate ap, by Merck Group (11 mentions)
Ammonium persulfate, by Merck Group (10 mentions)
Acrylamide, by Merck Group (8 mentions)
N n methylenebisacrylamide, by Merck Group (7 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (7 mentions)
Bovine serum albumin (bsa), by Merck Group (5 mentions)
Glycine, by Merck Group (4 mentions)
N n methylenebisacrylamide bis, by Merck Group (4 mentions)
β mercaptoethanol, by Merck Group (4 mentions)
Ammonium persulphate, by Merck Group (3 mentions)
Acrylic acid, by Merck Group (3 mentions)
Glutaraldehyde, by Merck Group (3 mentions)
Methanol, by Merck Group (3 mentions)
N n dimethylformamide (dmf), by Merck Group (3 mentions)
Ethanol, by Merck Group (3 mentions)
N isopropylacrylamide nipaam, by Merck Group (3 mentions)
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Potassium persulfate, by Merck Group (2 mentions)
Acryloyl chloride, by Merck Group (2 mentions)
Acetone, by Merck Group (2 mentions)
52 protocols using n n n n tetramethylethylenediamine temed
1
Docetaxel Polymer Conjugate Synthesis
2
Acrylamide Gel Preparation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acrylamide and bisAcrylamide solutions were purchased from Bio-Rad. N,N,N′,N′-Tetramethylethylenediamine (TEMED), N-[3-(Trimethoxysilyl)propyl]ethylene- diamine silane and dichlorodimethylsilane solution were purchased from Sigma. Sodium hydroxide (NaOH) and ammonium persulphate (APS) were purchased from Merck. Glutaraldehyde, ethanol and other solvents were purchased from Panreac AppliChem. Rectangular cover glasses were provided by Thermo Scientific. MEM (E15-888), L-Glutamine, fetal bovine serum and penicillin/streptomycin were purchased from PAA Laboratories (Pasching, Austria).
3
Synthesis and Characterization of PLCL Polymers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Poly-L-lactide-co-caprolactone (PLCL, 70% L-lactyde and 30% caprolactone) was purchased from Corbion Purac, the Netherlands. Chloroform (CHCl3) and N,N-dimethylformamide (DMF) were purchased from POCh, Poland. Bovine serum albumin conjugated with fluorescein (BSA-FITC), N,N isopropylacrylamide (NIPAAm), N,N’-methylene bisacrylamide (BIS-AAm), ammonium persulfate (APS), and N,N,N’,N’- tetramethylethylenediamine (TEMED) were purchased from Sigma Aldrich, Poland. Max Red Aqueous Fluorescent microsphere tracers dispersed in 1% (w/w) aqueous solution were purchased from Thermo Scientific™.
4
Synthesis of N-acryloylglycine amide (NAGA) Monomer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glycinamide hydrochloride (Bachem), potassium
carbonate (Fisher Scientific), acryloyl chloride (Sigma-Aldrich),
anhydrous diethyl ether (J.T. Baker), acetone, and methanol (both
from Sigma-Aldrich) were all used as received to prepare the NAGA
monomer according to the procedure described in the literature.26 (link)N,N,N′,N′-tetramethylethylenediamine
(TEMED) and sodium dodecyl sulfate (SDS) (both from Sigma) were used
as received. Ammonium persulfate (APS) and N,N′-methylenebis(acrylamide) (BIS) were recrystallized,
and MAA was distilled before use. Other substances and solvents with
the highest purity were used as received. Deionized water was used
in the syntheses and in the dialysis. For the dialysis, the membranes
had a molecular weight cutoff of 12–14 kDa.
carbonate (Fisher Scientific), acryloyl chloride (Sigma-Aldrich),
anhydrous diethyl ether (J.T. Baker), acetone, and methanol (both
from Sigma-Aldrich) were all used as received to prepare the NAGA
monomer according to the procedure described in the literature.26 (link)N,N,N′,N′-tetramethylethylenediamine
(TEMED) and sodium dodecyl sulfate (SDS) (both from Sigma) were used
as received. Ammonium persulfate (APS) and N,N′-methylenebis(acrylamide) (BIS) were recrystallized,
and MAA was distilled before use. Other substances and solvents with
the highest purity were used as received. Deionized water was used
in the syntheses and in the dialysis. For the dialysis, the membranes
had a molecular weight cutoff of 12–14 kDa.
5
Graphite Flakes Functionalization for Biomedical Applications
6
RecA Protein Purification and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RecA protein from E. coli at a concentration of 2 mg/mL was obtained from New England Biolabs Inc. (Ipswich, USA). SYBR Gold Nucleic Acid Gel Stain, XapI enzyme (recognition sequence 5′…R^AATTY…3′, 10 units/μL) and 1X tango buffer containing tris acetate (TAc) (33 mM, pH 7.9), magnesium acetate (MgAc) (10 mM) potassium acetate (66 mM), and BSA (0.1 mg/mL) were purchased from Thermo Fisher Scientific Inc. (Waltham, USA). Proteinase K (>800 units/mL), ammonium persulphate, TAc, MgAc (1 M), tris-EDTA (TE) buffer (100X), N,N,N′,N′-tetramethylethylenediamine (TEMED) and acrylamide/bis-acrylamide (30%) were obtained from Sigma-Aldrich (St. Louis, USA). Synthetic oligomers were purchased from Integrated DNA Technologies (Leuven, Belgium) and were stored at a concentration of 100 μM in 1x TE buffer at −20 °C. Blue/Orange loading dye (6X) was obtained from Promega Corporation (Madison, USA).
7
Hydrogel Synthesis and Functionalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
N-isopropylacrylamide (NIPAM; Wako Pure Chemical Industries Ltd.) was recrystallized from n-hexane before use. For hydrogel synthesis, N,N′-methylene-bis-acrylamide (MBA), ammonium peroxydisulfate, and N,N,N′,N′-tetramethyl ethylenediamine (TEMED) were used as a cross-linker, initiator, and accelerator, respectively, and they were purchased from Sigma-Aldrich and used as received. PSA polymer with a molecular weight of 800 kDa was obtained from Nippon Shokubai Pte Ltd. and used as received. Branched PEI (Sigma-Aldrich) with a molecular weight of 2 and 25 kDa, TMC (Sigma-Aldrich), and hexane were used for the synthesis of the PA layer on the hydrogel surface. Droplet production oil and stabilizer were obtained from Suzhou Rainsure Scientific Co. Ltd. Adenosine 5′-phosphosulfate, adenosine 5′-triphosphate sulfurylase, Bacillus stearothermophilus (Bst) polymerase v2.0 Warm Start, and 10× Bst buffer were purchased from New England Biolabs (NEB), Beijing, China. d -Luciferin sodium was obtained from Promega (Shanghai, China). Avian myeloblastosis virus Reverse Transcriptase was purchased from Woosen Biotechnology (Shanghai, China).
8
Synthesis and Testing of Glucose-Responsive Hydrogels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the synthesis [2 (link),32 (link)] of the hydrogel, acrylamide (AAM) was obtained from Fluka Analytical. N-[3 (dimethylamino)propyl]acrylamide (DMA) was purchased from Polysciences Inc. (Warrington, PA, USA), and 3-acrylamidophenylboronic acid (3-APB) was purchased from Frontier Scientific (Logan, UT, USA). N,N-methylenebisacrylamide (BIS), 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), dimethyl sulfoxide (DMSO), ammonium persulfate (APS), and N,N,N′,N′-tetramethylethylenediamine (TEMED) were purchased from Sigma-Aldrich (St. Louis, MO, USA). For hydrogel testing in a medium with high ionic concentrations, 1X-PBS was prepared from Dulbecco’s phosphate buffered saline powder with pH and ionic strength adjusted to 7.4 and 155 mM, respectively. For hydrogel testing of an analyte with low ionic concentration, deionized (DI) water was used for testing.
9
Biocompatible Polymer Nanoplatforms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To prepare the nanoplatforms, polycaprolactone (PCL, Mn 80 kDa), N,N-dimethylformamide (DMF), chloroform (99%), N,N-isopropylacrylamide (NIPAAm, 97%), N-isopropylomethacrylamide (NIPMAAm, 97%), N,N′-methylene bisacrylamide (BIS-AAm, 99.5%), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 98%), ammonium persulfate (APS, 98%), N,N,N′,N′-tetramethylethylenediamine (TEMED, 99%) were bought from Sigma-Aldrich (Poland). Gold nanorods (AuNRs, λ = 800 nm, OD = 50, C = 0.88 mg mL−1) from nanoComposix (USA) were used as received.
To perform biocompatibility tests, L929 murine fibroblasts, bovine serum albumin (BSA), phosphate buffer saline (PBS), Triton X, and DAPI were obtained from Sigma-Aldrich (Poland). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin (PS), and EDTA-trypsin were bought from Gibco Invitrogen (USA). Alexa Fluor 488 Phalloidin, PrestoBlue reagent and live/dead cytotoxicity kit assay were purchased from Thermo-Fisher Scientific (USA).
To perform biocompatibility tests, L929 murine fibroblasts, bovine serum albumin (BSA), phosphate buffer saline (PBS), Triton X, and DAPI were obtained from Sigma-Aldrich (Poland). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin (PS), and EDTA-trypsin were bought from Gibco Invitrogen (USA). Alexa Fluor 488 Phalloidin, PrestoBlue reagent and live/dead cytotoxicity kit assay were purchased from Thermo-Fisher Scientific (USA).
10
Synthesis and Characterization of Metal-Polymer Composites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemical reagents were of analytical grade and used as received without further purification. These reagents included 4-(dimethylamino)pyridine (DMAP, 99%, Sigma-Aldrich, Chile), glycidyl methacrylate (GMC, 97%, Sigma-Aldrich, Chile), dimethyl sulfoxide (DMSO, 99%, Sigma-Aldrich, Chile), 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS, 99%, Sigma-Aldrich, Chile), N,N-dimethylformamide (DMF, 99%, Sigma-Aldrich, Chile), benzoyl peroxide (BP, 98%, Sigma-Aldrich, Chile), N,N,N′,N′-tetramethylethylene-diamine (TEMED, 99%, Sigma-Aldrich, Chile), Cd(NO3)·4H2O (98%, Merck Chile), Cu(NO3)·3H2O (99%, Merck Chile), Pb(NO3)2 (99%, Merck Chile), NaOH (99%, Merck Chile), HNO3 (70%, Merck Chile), KOH (90%, Merck Chile), ethanol absolute (Merck Chile), methanol (Technical grade, Diprolab), and HCl fuming (37%, Merck Chile).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!