Unity plus 500

Manufactured by Agilent Technologies

The Unity Plus 500 is a laboratory equipment product offered by Agilent Technologies. It is designed for general laboratory use, providing essential functions and capabilities without extensive detail or interpretation.

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Lab products found in correlation

2400 series 2 chn elemental analyzer, by PerkinElmer (2 mentions) Poroshell ec c18, by Agilent Technologies (1 mentions) Fluka silica gel 60, by Merck Group (1 mentions) Kieselgel 60 f254, by Merck Group (1 mentions) Vxr 500, by Agilent Technologies (1 mentions) Silica gel 60, by Kanto Chemical (1 mentions) V 560 uv vis spectrophotometer, by Jasco (1 mentions) Pu 2086 pump, by Jasco (1 mentions) Pack diol 120 np, by YMC (1 mentions) Jms 700 mstation, by JEOL (1 mentions) Uv 970 detector, by Jasco (1 mentions) Ri 2031 detector, by Jasco (1 mentions) Cosmosil 5sl 2, by Nacalai Tesque (1 mentions) Kieselgel 60 f254 plate, by Merck Group (1 mentions) Triple tof 5600 system, by AB Sciex (1 mentions) Cary 60 spectrophotometer, by Agilent Technologies (1 mentions) J 715 spectropolarimeter, by Jasco (1 mentions)

7 protocols using unity plus 500

NMR and Melting Point Analysis

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¹H NMR spectra were recorded on a Varian VXR-500 or Varian Unity-500-Plus spectrometer (499.74 MHz) and referenced to residual solvent signals. ¹¹B NMR spectra were recorded on a Varian VXR-500 spectrometer operating at 160.41 MHz. ³¹P NMR spectra were recorded on a Varian Unity-500-Plus spectrometer operating at 202.29 MHz. ¹¹B and ³¹P NMR spectra were indirectly referenced relative to SiMe₄ according to the IUPAC standard using VnmrJ software. All coupling constants are apparent J values measured at the indicated field strengths. Melting points were measured on a MEL-TEMP or Thomas-Hoover capillary melting apparatus and are uncorrected.
Note: because all of the compounds are in equilibrium with each other, it was not possible to isolate bulk samples containing one species for combustion analysis.

Ghaffari B., Vanchura BA I.I., Chotana G.A., Staples R.J., Holmes D., Maleczka RE J.r, & Smith MR I.I.I. (2015). Reversible Borylene Formation from Ring Opening of Pinacolborane and Other Intermediates Generated from Five-Coordinate Tris-Boryl Complexes: Implications for Catalytic C–H Borylation. Organometallics, 34(19), 4732-4740.

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Chromatographic Purification and Analysis of Compounds

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All reactions were performed in inert atmosphere with magnetic stirring. DMF was purified by distillation from benzene/water. The reactions were monitored by TLC on Merck F254 silica gel precoated plates. The following solvent systems (by volume) were used for TLC development: CH₂Cl₂:MeOH:CH₃COOH (15:1:0.1, v/v/v) (A), CH₂Cl₂:MeOH (50:1, v/v) (B), CHCl₃:MeOH:NH₃ (9:1:0.2, v/v/v) (C), CHCl₃:MeOH:NH₃ (5:1:0.2, v/v/v) (D).¹H NMR and ¹³C NMR spectra were taken on the camera Varian Unity 500 Plus in CDCl₃, CD₃OD or DMSO. Mass spectra were performed at the Laboratory of Mass Spectrometry MALDI-TOF on the matrix DHB (BIFLEX III Bruker).
Conditions of chromatographic HPLC separation and detection of examined compounds (11–24, 27, 28): column - Poroshell EC-C18 (3.0 × 150 mm), 2.7 μm, Agilent Technologies; column temperature - 40 °C; injection volume - 2 μL; flow rate - 0.4 mL/min; eluents: (A) 0.1% HCOOH in water, (B) 0.1% HCOOH in ACN/MeOH (1:1, v/v); gradient program (Table 5):Table 5

Gradient program for HPLC.

Table 5

Time [min]	% A	% B
0	90	10
20	0	100
30	0	100

Post time - 10 min; UV–Vis detection; wavelengths UV: 254 nm; Vis: 580 nm; peak width > 0.1 min (2s); ESI MS detection.

Siebert A., Wysocka M., Krawczyk B., Cholewiński G, & Rachoń J. (2017). Synthesis and antimicrobial activity of amino acid and peptide derivatives of mycophenolic acid. European Journal of Medicinal Chemistry, 143, 646-655.

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Synthesis of Substituted Sulfonamide Derivatives

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The following instruments and parameters were used: melting points Boetius PHMK apparatus; IR spectra: KBr pellets, 400–4000 cm^–1 Thermo Mattson Satellite FTIR spectrophotometer; ¹H and ¹³C NMR: Varian Unity 500 plus apparatus at 500 MHz; chemical shifts are expressed in parts per million (ppm) relative to TMS as internal standard. Elemental analyses for C, H, and N were performed on a 2400 Series II CHN Elemental Analyzer (Perkin Elmer, Shelton, CT, USA) and are in agreement with the theoretical values within ±0.4% range. Thin-layer chromatography (TLC) was performed on Merck Kieselgel 60 F₂₅₄ plates and visualized with UV. Gravity column chromatography was performed on Fluka silica gel 60 of particle size 35–75 μm and 220–440 mesh (Sigma–Aldrich Chemie, Steinheim, Germany). The starting compounds (4–chloro–2–mercapto–5-methylbenzenesulfonamide (1)) [33 ] and cinnamic acid derivatives (10–15) were synthesized according to the literature sources [34 (link),35 (link),36 (link)].

Bułakowska A., Sławiński J., Hałasa R., Hering A., Gucwa M., Ochocka J.R, & Stefanowicz-Hajduk J. (2023). An In Vitro Antimicrobial, Anticancer and Antioxidant Activity of N–[(2–Arylmethylthio)phenylsulfonyl]cinnamamide Derivatives. Molecules, 28(7), 3087.

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Spectroscopic Analysis of Organic Compounds

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A JASCO P1020 NK digital polarimeter was used to measure the optical rotations. The diffuse reflectance method was used to record IR spectra on a JASCO FT/IR-410 spectrophotometer. UV spectra were obtained on a JASCO V-560 UV/Vis spectrophotometer. ECD spectra were measured on a JASCO J-725N spectrophotometer. ¹H and ¹³C NMR spectra were recorded on a JEOL JNM-AL 400 (¹H: 400 MHz) or Varian Unity plus 500 (¹H: 500 MHz, ¹³C: 126 MHz, respectively) spectrometer using CDCl₃. Chemical shift values are given in δ (ppm), using the solvent peak signals (CDCl₃: TMS) as references, and coupling constants (J) are reported in Hz. A JEOL JMS-700 MStation was used to record mass spectra, including high-resolution spectra. Column chromatography was performed on Silica gel 60 (100–210 mesh, Kanto Chemical Co., Inc., Tokyo, Japan). Preparative HPLC was performed on a JASCO chromatograph (n-hexane–EtOAc, CHCl₃–EtOAc) equipped with a JASCO PU-2086 pump, a JASCO UV-970 detector, a JASCO RI-2031 detector, and various columns: COSMOSIL 5SL-II (10 × 250 mm, Nacalai Tesque Inc., Kyoto, Japan), COSMOSIL 5SL-II (4.6 × 250 mm, Nacalai Tesque Inc., Kyoto, Japan), YMC-Pack Diol-120-NP (4.6 × 250 mm, YMC Co., Ltd., Kyoto, Japan), and Inertsil CN-3 (4.6 × 250 mm, GL Sciences, Tokyo, Japan).

Hu Y., Saito Y., Matsuo Y., Gong X, & Tanaka T. (2022). New Benzofuran Oligomers from the Roots of Eupatorium heterophyllum Collected in China. Molecules, 27(24), 8856.

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Characterization of Organic Compounds

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Melting points were measured using Boethius PHMK apparatus. IR spectra were measured on Thermo Mattson Satellite FTIR spectrometer in KBr pellets; an absorption range was 400–4000 cm⁻¹. ¹H NMR and ¹³C NMR spectra were recorded on a Varian Gemini 200 apparatus or Varian Unity Plus 500 apparatus. Chemical shifts are expressed at δ values relative to Me₄Si (TMS) as an internal standard. The apparent resonance multiplicity is described as: s (singlet), d (doublet), dd (doublet of doublets), t (triplet), m (multiplet), and br (broad) signal. The addition of equimolar TFA was necessary to obtain ¹³C NMR spectra. Due to a poor solubility of compounds 21 and 23, the obtained ¹³C NMR spectra were not sufficient. HRMS analyses were performed on a TripleTOF 5600 + System (AB SCIEX, USA) in positive ion mode. Elemental analyses were performed on PerkinElmer 2400 Series II CHN Elemental Analyzer and the results were within ± 0.4% of the theoretical values. Thin-layer chromatography (TLC) was performed on Merck Kieselgel 60 F254 plates and visualized with UV. The commercially unavailable monopotassium salts were obtained according to the following methods described previously: 1, 4 [49 ], 2 [50 (link)], 3–5, 8 [51 (link)], and 7 [52 ].

Pogorzelska A., Żołnowska B., Sławiński J., Kawiak A., Szafrański K., Belka M, & Bączek T. (2018). Synthesis of 2-alkylthio-N-(quinazolin-2-yl)benzenesulfonamide derivatives: anticancer activity, QSAR studies, and metabolic stability. Monatshefte Fur Chemie, 149(10), 1885-1898.

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1H NMR Spectroscopy in D2O

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1 H NMR spectra were recorded in D2O at 25 °C with a Varian Unity Plus 500 spectrometer at 499.9 MHz using standard pulse programs from the Varian library. Two-dimensional (2D) experiments were acquired using 1 K data points and 256 increments. Spectra were referred to the solvent signal.

Falcone E., Ahmed I.M.M., Oliveri V., Bellia F., Vileno B., El Khoury Y., Hellwig P., Faller P, & Vecchio G. (2020). Acrolein and Copper as Competitive Effectors of α-Synuclein. Chemistry (Weinheim an der Bergstrasse, Germany), 26(8).

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Spectroscopic Characterization of Compounds

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UV/Vis: Varian Cary 60 spectrophotometer; λ_max in nm (log ϵ). CD‐spectra: JASCO J‐715 spectropolarimeter; λ_max and λ_min in nm (Δϵ). ¹H NMR: Varian UNITY plus 500; δ in ppm with δ(CHD₂OD)=3.31 ppm, δ(CHCl₃)=7.26 ppm, δ(CD₃SOCD₂H)=2.50 ppm, coupling constant J_HH in Hz; ¹³C‐NMR: chemical shift values and signal assignments from ¹H,¹³C‐HSQC and ¹H,¹³C‐HMBC spectra. ESI‐MS: Finnigan LCQ Classic, ESI source, positive‐ion mode, flow rate 2 mL min⁻¹, solvent water/MeOH. FAB‐MS: Finnigan MAT‐95, positive‐ion mode, glycerin matrix; m/z (rel. intensity %). Analytical HPLC: Gynkotek “high‐precision pump” 480 G with vacuum online degasser, Gynkotek diode array detector DA340, all chromatograms were taken at room temperature, Phenomenex, ODS‐Hypersil 5 μ, 250×4.6 mm i.d. precolumn was used with a flow rate: 0.5 mL min⁻¹; solvent A: 50 mm aq. potassium phosphate (pH 7.0), solvent B: MeOH, solvent C: H₂O; solvent composition (A/B/C): 0–5 min: 60/40/0; 5–15 min: from 60/40/0 to 30/70/0; 15–25 min: from 30/70/0 to 0/100/0; 25–35 min: from 0/100/0; 35–37 min: 0/100/0 to 0/90/10; 37–42 min: from 0/90/10 to 60/40/0.

Li C., Wurst K., Berghold J., Podewitz M., Liedl K.R, & Kräutler B. (2018). Pyro‐Phyllobilins: Elusive Chlorophyll Catabolites Lacking a Critical Carboxylate Function of the Natural Chlorophylls. Chemistry (Weinheim an Der Bergstrasse, Germany), 24(12), 2987-2998.

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