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2 protocols using anti phosphoglucose mutase 1

1

Protein Expression Analysis of Tumor Samples

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Cells and tumor samples were subjected to lysis using a standard lysis buffer containing NP-40 and 0.1% TritonX-100. The antibodies used in this study included anti-Flag (Sigma-Aldrich, clone M2, catalog # F1804-1MG), anti-actin (Sigma-Aldrich, catalog # A2066), anti-hexokinase 2 (Cell Signaling Technology, catalog # 2867), anti-aldolase (Cell Signaling Technology, catalog # 3188), anti-pyruvate kinase M2 (Cell Signaling Technology, catalog # 3198), anti-lactate dehydrogenase A (Cell Signaling Technology, catalog # 3582; Epitomics, catalog # 3468-1), anti-pyruvate dehydrogenase kinase 1 (Zymed, catalog # 34-9500), anti-enolase 1 (Cell Signaling Technology, catalog # 3810), anti-glutaminase (Abcam, catalog # ab60709), anti-phosphofructose kinase 1 (Novus Biologicals, catalog # NBP1-19585), anti-phosphoglucose mutase 1 (Abcam, catalog # ab55616), anti-14-3-3σ (Fitzgerald Industries International, catalog # 10R-S101a), and anti-c-Myc (Abcam, clone 9E10, catalog # ab32). Original films of immunoblotting were in supplementary Figures 24–29.
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2

Protein Expression Analysis of Tumor Samples

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Cells and tumor samples were subjected to lysis using a standard lysis buffer containing NP-40 and 0.1% TritonX-100. The antibodies used in this study included anti-Flag (Sigma-Aldrich, clone M2, catalog # F1804-1MG), anti-actin (Sigma-Aldrich, catalog # A2066), anti-hexokinase 2 (Cell Signaling Technology, catalog # 2867), anti-aldolase (Cell Signaling Technology, catalog # 3188), anti-pyruvate kinase M2 (Cell Signaling Technology, catalog # 3198), anti-lactate dehydrogenase A (Cell Signaling Technology, catalog # 3582; Epitomics, catalog # 3468-1), anti-pyruvate dehydrogenase kinase 1 (Zymed, catalog # 34-9500), anti-enolase 1 (Cell Signaling Technology, catalog # 3810), anti-glutaminase (Abcam, catalog # ab60709), anti-phosphofructose kinase 1 (Novus Biologicals, catalog # NBP1-19585), anti-phosphoglucose mutase 1 (Abcam, catalog # ab55616), anti-14-3-3σ (Fitzgerald Industries International, catalog # 10R-S101a), and anti-c-Myc (Abcam, clone 9E10, catalog # ab32). Original films of immunoblotting were in supplementary Figures 24–29.
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