2.5 × 105 AT-MSCs in 100 µl PBS per injection were intradermally injected to the both sides of shaved dorsal skin of mice. Mice intradermally injected with 100 µl PBS served as controls. To induce IC-mediated vasculitis, mice were challenged with the Arthus reaction [25 (link), 26 (link)]. Briefly, the Arthus reaction was elicited by i.v. injection of 100 µl of 2% bovine serum albumin (BSA) and 1% Evans blue (Sigma-Aldrich) in PBS. Afterward 40 µl of rabbit anti-BSA antibody (Sigma-Aldrich) at the concentration of 4.8 mg/ml was injected intradermally into the dorsal skin that had been injected with AT-MSCs or PBS. Rabbit IgG served as the background control. Mice were sacrificed 4 hours after the Arthus reaction and skin specimen were harvested and digitally photographed. The areas of Evans blue spots on the skin, representing the areas of hemorrhage due to IC-mediated vasculitis, were analyzed by Image J.
Rabbit anti bsa antibody
Manufactured by Merck Group
Rabbit anti-BSA antibody is a laboratory reagent used in various immunological and biochemical applications. It is a polyclonal antibody produced by immunizing rabbits with bovine serum albumin (BSA). This antibody can be used to detect and quantify BSA in samples.
Automatically generated - may contain errors
3 protocols using rabbit anti bsa antibody
1
Intradermal AT-MSC Injection Attenuates Arthus Reaction
2
Carbamylated Protein Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Carbamylated FCS (CarFCS) and unmodified FCS (UmFCS), or carbamylated BSA (CarBSA) and unmodified BSA (UmBSA) were electrophoresed and transferred onto nitrocellulose membranes. Membranes were incubated for 5 min in bullet blocking one for western blotting (Nacalai Tesque) or for 30 min in 5% skimmed milk in PBS with 0.05% Tween 20 (PBS-T); the former was for sera from patients and the latter for anti-BSA antibody, described below.
The membranes were incubated for 1 h in sera from patients or rabbit anti-BSA antibody (Sigma-Aldrich) diluted 1000-fold by each blocking buffer followed by three washes in PBS-T. Sera were obtained from seven RA patients with anti-CarP antibodies. Subsequently, they were incubated for 1 h in horseradish peroxidase-conjugated anti-human or anti-rabbit IgG (Promega, Madison, WI, USA) diluted 10 000-fold by each blocking buffer. Finally, they were washed three times, and antibodies were detected by SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA, USA). All the procedures were conducted at room temperature.
The membranes were incubated for 1 h in sera from patients or rabbit anti-BSA antibody (Sigma-Aldrich) diluted 1000-fold by each blocking buffer followed by three washes in PBS-T. Sera were obtained from seven RA patients with anti-CarP antibodies. Subsequently, they were incubated for 1 h in horseradish peroxidase-conjugated anti-human or anti-rabbit IgG (Promega, Madison, WI, USA) diluted 10 000-fold by each blocking buffer. Finally, they were washed three times, and antibodies were detected by SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA, USA). All the procedures were conducted at room temperature.
3
Intradermal AT-MSC Injection Attenuates Arthus Reaction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2.5 × 105 AT-MSCs in 100 µl PBS per injection were intradermally injected to the both sides of shaved dorsal skin of mice. Mice intradermally injected with 100 µl PBS served as controls. To induce IC-mediated vasculitis, mice were challenged with the Arthus reaction [25 (link), 26 (link)]. Briefly, the Arthus reaction was elicited by i.v. injection of 100 µl of 2% bovine serum albumin (BSA) and 1% Evans blue (Sigma-Aldrich) in PBS. Afterward 40 µl of rabbit anti-BSA antibody (Sigma-Aldrich) at the concentration of 4.8 mg/ml was injected intradermally into the dorsal skin that had been injected with AT-MSCs or PBS. Rabbit IgG served as the background control. Mice were sacrificed 4 hours after the Arthus reaction and skin specimen were harvested and digitally photographed. The areas of Evans blue spots on the skin, representing the areas of hemorrhage due to IC-mediated vasculitis, were analyzed by Image J.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!