Gentamicin sulfate

Manufactured by Nacalai Tesque

Sourced in Japan

Gentamicin sulfate is a laboratory compound used as a microbiological culture medium ingredient. It is an antibiotic that inhibits the growth of certain bacteria.

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Lab products found in correlation

Kh2po4, by Nacalai Tesque (2 mentions) Cacl2 2h2o, by Nacalai Tesque (2 mentions) Sodium chloride (nacl), by Nacalai Tesque (1 mentions) Potassium chloride (kcl), by Nacalai Tesque (1 mentions) Mgcl2 6h2o, by Nacalai Tesque (1 mentions) Human chorionic gonadotropin (hcg), by Aska Pharmaceutical (1 mentions) Hygromycin b, by Fujifilm (1 mentions) 5 ethynyl 2 deoxyuridine edu, by Thermo Fisher Scientific (1 mentions) Dmem low glucose, by Merck Group (1 mentions) Hanks balanced salt solution (hbss), by Fujifilm (1 mentions) Paraffin liquid, by Nacalai Tesque (1 mentions) Sodium hydroxide (naoh), by Nacalai Tesque (1 mentions) Medium 199, by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Nacalai Tesque (1 mentions) Mueller hinton agar (mha), by Merck Group (1 mentions) Mueller hinton broth (mhb), by Merck Group (1 mentions) Low molecular weight chitosan, by Merck Group (1 mentions) Phosphate buffer saline tablets, by Thermo Fisher Scientific (1 mentions) Gold 3 chloride hydrate, by Merck Group (1 mentions) Human serum albumin (hsa), by CSL Behring (1 mentions)

Spelling variants of this product

Gentamicin (26 citations) Gentamicin sulfate solution (3 citations)

7 protocols using gentamicin sulfate

Porcine Oocyte Maturation Protocol

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NaCl, KCl, KH₂PO₄, MgCl₂·6H₂O, CaCl₂·2H₂O, and gentamicin sulfate were purchased from Nacalai
Tesque (Kyoto, Japan). MgSO₄·7H₂O was purchased from Ishizu Pharmaceutical (Osaka, Japan). Furthermore, eCG (the trade name; Serotropin)
and hCG (the trade name; Gonatropin) were purchased from ASKA Pharmaceutical (Tokyo, Japan). Unless otherwise specified, other chemicals were purchased from
Sigma Aldrich (St. Louis, MO, USA).
Modified TL-HEPES-PVA medium composed of 114 mM NaCl, 3.2 mM KCl, 2 mM NaHCO₃, 0.34 mM NaH₂PO₄, 10 mM Na-lactate, 0.5 mM
MgCl₂·6H₂O, 2 mM CaCl₂·2H₂O, 12 mM sorbitol, 10 mM HEPES, 0.2 mM Na-pyruvate, 0.1% (w/v) polyvinyl alcohol (PVA),
25 µg/ml gentamicin sulfate, and 65 µg/ml potassium penicillin G was used for collecting and washing COCs. The basic IVM medium was a BSA-free,
chemically-defined, porcine oocyte medium (POM, Research Institute for the Functional Peptides, Yamagata, Japan) supplemented with 50 µM beta-mercaptoethanol
(mPOM) [20 (link)]. This IVM medium was equilibrated at 39°C in an atmosphere of 5% CO₂ overnight prior to use.

OKUDAIRA Y., WAKAI T, & FUNAHASHI H. (2017). Levels of cyclic-AMP and cyclic-GMP in porcine oocyte-cumulus complexes and cumulus-free oocytes derived from small and middle follicles during the first 24-hour period of in vitro maturation. The Journal of Reproduction and Development, 63(2), 191-197.

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Transformation of Marchantia polymorpha

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Transformation of M. polymorpha was performed as described previously [8 (link)]. For simultaneous transformation with multiple vectors, the respective Agrobacterium cultures were mixed at a 1:1 ratio. Half-strength Gamborg’s B5 medium containing 0.5 g/L MES and 1% (w/v) agar and supplemented with hygromycin B (Wako Pure Chemical Industries, Osaka, Japan), gentamicin sulfate (Nacalai Tesque, Kyoto, Japan), CS (kindly provided by Dupont, Wilmington, DE, USA), or G418 (Nacalai Tesque) was used to select transformants. The stock solution of CS (50 mM) was prepared with DMSO.

Ishizaki K., Nishihama R., Ueda M., Inoue K., Ishida S., Nishimura Y., Shikanai T, & Kohchi T. (2015). Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. PLoS ONE, 10(9), e0138876.

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Retinal Cell Culture Protocol

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The eyeballs were dissected and washed with Hank’s Balanced Salt Solution (HBSS (−), FUJIFILM Wako Chemicals, Osaka, Japan) containing 0.1% Gentamicin Sulfate (Nacalai Tesque, Kyoto, Japan). After incubating in HBSS(−)/Gentamicin at 37 °C for 1 h, the retinas were isolated, mechanically dissociated by pipetting, and centrifuged at 1000 rpm for 10 min. The pellet was resuspended in Dulbecco’s Modified Eagle Medium (DMEM) low glucose (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) and 1 µl/ml Penicillin–Streptomycin (PS), plated on polylysine-coated coverslips in 12-well dishes and cultured at 37 °C in a 5% CO₂ incubator. To label mitotic cells, 5-ethynyl-2′-deoxyuridine (EdU, Thermo Fisher Scientific, Waltham, MA, USA) was added to the medium (8 μl/ml) after 1 day of culture.

Kato M., Sudou N., Nomura-Komoike K., Iida T, & Fujieda H. (2022). Age- and cell cycle-related expression patterns of transcription factors and cell cycle regulators in Müller glia. Scientific Reports, 12, 19584.

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Porcine Oocyte Maturation and Culture

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Sodium chloride (NaCl), KCl, KH₂PO₄, CaCl₂-2H₂O, HCl, NaOH, gentamicin-sulfate, and liquid paraffin were purchased from Nacalai Tesque (Kyoto,
Japan). Equine chorionic gonadotropin (eCG; Serotropin) and human chorionic gonadotropin (hCG; Gonadotropin) were obtained from ASKA Pharmaceutical (Tokyo, Japan). Unless specified, all
other chemicals were purchased from Sigma Aldrich (St. Louis, MO, USA).
The medium used for collecting and washing cumulus-oocyte complexes (COCs) was modified HEPES-buffered Tyrode’s lactate containing polyvinyl alcohol (TL-HEPES-PVA) [20 (link)]. The basic IVM medium was BSA-free chemically defined medium, porcine oocyte medium (POM, Research Institute for the Functional Peptides, Yamagata, Japan) modified
with 50 μM beta-mercaptoethanol (mPOM) [20 (link)]. The medium for the transit culture following parthenogenetic activation was, Medium-199 (Invitrogen,
Carlsbad, CA, USA) modified with 3.05 mM glucose, 2.92 mM Hemi-calcium lactate, 0.91 mM Na-pyruvate, 12 mM sorbitol, 75 μg/ml potassium penicillin G, and 25 μg/ml gentamicin (mM199) [21 (link)]. The chemically defined medium for in vitro development to the blastocyst stage was porcine zygote medium (PZM-5; Research Institute
for the Functional Peptides) [22 (link)]. All media, except TL-HEPES-PVA, were equilibrated overnight at 39ºC in an atmosphere of 5% CO₂ in the air
before use.

KOHATA-ONO C., WAKAI T, & FUNAHASHI H. (2019). The autophagic inducer and inhibitor display different activities on the meiotic and developmental competencies of porcine oocytes derived from small and medium follicles. The Journal of Reproduction and Development, 65(6), 527-532.

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Antibacterial Potential of Lignas Sclerotial Powder

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L. rhinocerotis sclerotial powder was provided as a gift from Lignas Bio Synergy, Selangor, Malaysia. Gold (III) chloride hydrate (99.999% trace metals basis) was procured from Sigma-Aldrich (Malaysia). Low molecular weight (LMW) chitosan (molecular weight of 190 kDa, 75–85% degree of deacetylation) was purchased from Sigma-Aldrich (Ireland). Glacial acetic acid (99.7% purity) was purchased from R&M Chemicals, UK. Distilled water was produced in the laboratory using a Hamilton WCS/85 cabinet water still. For antibacterial tests, four bacterial strains (S. aureus, P. aeruginosa, Bacillus sp., and E. coli) were obtained from Microbiologic Laboratory of the Faculty of Pharmacy, Universiti Kebangsaan Malaysia (Kuala Lumpur, Malaysia). Mueller-Hinton broth (MHB) and Mueller-Hinton agar (MHA) were purchased from Merck, Germany. Phosphate buffer saline (PBS) tablets were purchased from Invitrogen, USA. Gentamicin sulfate was purchased from Nacalai Tesque, Japan. All reagents were of analytical grade.

Katas H., Lim C.S., Nor Azlan A.Y., Buang F, & Mh Busra M.F. (2018). Antibacterial activity of biosynthesized gold nanoparticles using biomolecules from Lignosus rhinocerotis and chitosan. Saudi Pharmaceutical Journal : SPJ, 27(2), 283-292.

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SARS-CoV-2 Virus Isolation and Culture

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Cell culture and virus isolation were performed according to the method of Matsuyama et al.[14] (link). VeroE6/TMPRSS2 (JCRB1819) cells were obtained from the National Institutes of Biomedical Innovation, Health and Nutrition, Japan. The cells were maintained in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal calf serum (FCS) and antibiotics at 37°C with 5% CO₂.
A 24-well plate containing a VeroE6/TMPRSS2 cell culture monolayer at a low crowding density (70-90% confluence) was prepared. After the medium was discarded, 100 µl of the undiluted sample or 10-fold dilutions of the sample were added to the cells and incubated for 1 hour at 37°C. Then, 0.5 ml of DMEM with 5% FCS, 2.5 µg/ml amphotericin B (Sigma-Aldrich Japan G.K., Tokyo, Japan), and 50 µg/ml gentamicin sulfate (Nacalai Tesque, Kyoto, Japan) was added and incubated at 37°C for 1 week until a cytopathogenic effect was observed.

Saitoh H., Sakai-Tagawa Y., Nagasawa S., Torimitsu S., Kubota K., Hirata Y., Iwatsuki-Horimoto K., Motomura A., Ishii N., Okaba K., Horioka K., Abe H., Ikemura M., Rokutan H., Hinata M., Iwasaki A., Yasunaga Y., Nakajima M., Yamaguchi R., Tsuneya S., Kira K., Kobayashi S., Inokuchi G., Chiba F., Hoshioka Y., Mori A., Yamamoto I., Nakagawa K., Katano H., Iida S., Suzuki T., Akitomi S., Hasegawa I., Ushiku T., Yajima D., Iwase H., Makino Y, & Kawaoka Y. (2023). High titers of infectious SARS-CoV-2 in corpses of patients with COVID-19. International Journal of Infectious Diseases, 129, 103-109.

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Tumor Infiltrating Lymphocyte Isolation

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Obtained tumor fragments were washed in TIL buffer consisting of a 50/50 mix of RPMI1640 (Sigma-Aldrich)/D-PBS (Nacalai) supplemented with 1 mM EDTA (Nacalai), 0.5% human serum albumin (CSL Behring), 1% penicillin–streptomycin–amphotericin B suspension (Wako) or 1% antibiotic–antimycotic mixed stock solution (Nacalai), and 50 ng/ml gentamicin sulfate (Nacalai). Tumor fragments were minced with scissors, and the slurry was filtered with a 100-µm cell strainer (Greiner). A part of the cell suspensions passing through the cell strainer was characterized for flow cytometry. The residual cell suspensions were used for the TIL expansion with cytokine cocktails.

Ito T., Kawai Y., Yasui Y., Iriguchi S., Minagawa A., Ishii T., Miyoshi H., Taketo M.M., Kawada K., Obama K., Sakai Y, & Kaneko S. (2021). The therapeutic potential of multiclonal tumoricidal T cells derived from tumor infiltrating lymphocyte-derived iPS cells. Communications Biology, 4, 694.

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