Cells were transiently transfected with scrambled siRNA (catalog # sc-37007), GFRα1 siRNA (catalog # sc-35469), RET siRNA (catalog # sc-36404) (all from Santa Cruz) or p70S6K siRNA (catalog # #6572, Cell signaling) using the Lipofectamine 2000 transfection reagent (Invitrogen). Twenty-four or 48 hours after transfection, cells were used for further analyses.
P70s6k sirna
Manufactured by Cell Signaling Technology
P70S6K siRNA is a small interfering RNA (siRNA) targeting the p70 S6 kinase (P70S6K) gene. P70S6K is a serine/threonine protein kinase that plays a role in the regulation of cell growth and proliferation. The P70S6K siRNA can be used to knockdown the expression of the P70S6K gene in cellular systems.
Automatically generated - may contain errors
2 protocols using p70s6k sirna
1
Silencing Signaling Pathway Genes
2
Silencing S6K and eIF4E in Bladder Cancer Cells
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p70S6K siRNA (ID#: 6566, sense strand: 5′-GUGCCAAUCAGGUCUUUCU-3′, antisense strand: 5′-AGAAAGACCUGAUUGGCAC-3′) and eIF4E siRNA (ID#: 6311, sense strand: 5′-GGAUGGUAUUGAGCCUAUG-3′, antisense strand: 5′-CAUAGGCUCAAUACCAUCC-3′) were purchased from Cell Signaling Technologies. S6K siRNA (ID#: sc92312, sense strand 5′-CCUUCAACCACUAUCAGAAUU-3′, antisense strand: 5′-UUCUGAUAGUGGUUGAAGGUU-3′) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Transient transfection of 5637 and T24 cells was performed using Lipofectamine® 2000 reagent (Invitrogen) according to the manufacturer's instructions.
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