Four micrometer sections, cut from a 4-mm punch biopsy that were fixed in 10% buffered formalin and embedded in paraffin, were stained with hematoxylin and eosin (H&E) or labeled immunohistochemically with antibodies against CD4 (clone EP204, Epitomics, Cambridge, MA), CD8 (clone 4B11, Leica Biosystems Inc., Buffalo Grove, IL), CD56 (clone 56C04 Thermo-Scientific, Waltham, MA), CD68 (clone PG-M1, Dako, Carpinteria, CA) and CD138 (clone B-A38 Cell Marque, Rocklin, CA) using an automated immunohistochemistry staining platform (Bond Max, Leica-Microsystems, Buffalo Grove, IL). IHC detection of Tp was performed manually as previously described (10 (link)) using a rabbit polyclonal anti-Tp Ab (Biocare, Concord, CA, USA). Skin specimens from healthy volunteers of the same socioeconomic background and conditions were not available for analysis. Tissue known to contain Tp were used for the positive control of Tp IHCs. Additionally, secondary alone controls were used to assess any non-specific Ab binding for each Ab used.
Cd138 clone b a38
Manufactured by Cell Marque
Sourced in United States
CD138 (clone B-A38) is a laboratory reagent used for the detection of CD138 (Syndecan-1) in biological samples. CD138 is a cell surface proteoglycan that plays a role in cell-cell and cell-matrix interactions. This reagent can be used in various immunohistochemical and flow cytometry applications to identify and characterize cells expressing CD138.
Automatically generated - may contain errors
3 protocols using cd138 clone b a38
1
Immunohistochemical Profiling of Skin Biopsies
2
Immunohistochemical Analysis of Lymph Nodes
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Archived paraffin-embedded human cervical lymph nodes from patients with head and neck cancer who had undergone lymph node dissection as part of their routine patient care were stained for anti-human CD169 (clone HSn 7D2, In Vitro Technologies) and CD138 (clone B-A38, Cell Marque) according to the manufacturers protocols.
3
Immunohistochemical Characterization of Cancer Markers
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Formalin-xed, para n-embedded tissue blocks of the resected and biopsied specimens were cut into 3µm-thick sections, depara nized, and rehydrated. Each section was used for immunostaining. Immunohistochemical analyses were performed using an autostainer (Benchmark XT system; Roche Diagnostics, Basel, Switzerland) according to the manufacturer's instructions. The primary antibodies used in this study were as follows: a mouse monoclonal antibody against CD138 (clone B-A38, Cell Marque, CA, USA); a mouse monoclonal antibody against E-cadherin (clone 36, Roche Diagnostics); a mouse monoclonal antibody against N-cadherin (clone 6G11, DAKO Japan Co. Ltd., Kyoto, Japan); a rabbit polyclonal antibody against Slug (ab38551, Abcam, Cambridge, UK); and a rabbit polyclonal antibody against Snail (ab180714, Abcam).
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