Conducting of pathscan array was strictly followed the manufacturer's protocol. In brief, the glass slides containing detecting antibody matrix was prepared and assembled. Then the blocking buffer was added in each well and incubated in room temperature for 15 minutes. After washing, a total protein of 30μg was added in each well and incubated at 4°C overnight on an orbital shaker. Then the slide was washed with PBS and placed on a plastic dish. The images were captured using an Oddessy fluorescent imaging system (LI-COR Biotechnology, Lincoln, NE, USA).
Oddessy fluorescent imaging system
Manufactured by LI COR
Sourced in United States
The Odyssey fluorescent imaging system is a versatile instrument designed for the detection and quantification of fluorescent signals in a variety of biological samples. It utilizes high-sensitivity fluorescence detection technology to enable the visualization and analysis of proteins, nucleic acids, and other biomolecules labeled with fluorescent dyes or tags.
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Lab products found in correlation
2 protocols using oddessy fluorescent imaging system
1
Pathscan Array Protein Detection
2
Pathscan Array Protocol for Protein Detection
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Conducting of pathscan array was strictly followed the manufacturer’s protocol. In brief, the glass slides containing detecting antibody matrix was prepared and assembled. Then the blocking buffer was added in each well and incubated in room temperature for 15 minutes. After washing, a total protein of 30μg was added in each well and incubated at 4°C overnight on an orbital shaker. Then the slide was washed with PBS and placed on a plastic dish. The fluorescence intensity was captured using an Oddessy fluorescent imaging system (LI-COR Biotechnology, Lincoln, NE, USA).
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