Quantum dot conjugation kit

All chemicals and reagents purchased from sigma Aldrich and Daejung chemicals South Korea, and TCI chemicals, Japan without further purifications. Absorption spectra and drug release study were recorded in 1 cm quartz cuvettes using Evolution™ 60 UV–visible Spectrophotometer (Thermo Fischer Scientific, USA). Emission spectra were obtained on a Jasco-FP 6500 spectrofluorometer. Particle size was analyzed by Dynamic Light Scattering Spectrophotometer (Otsuka Electronics Co., Ltd, Japan). Zeta potential was measured by electrophoretic light scattering (ELS) spectrometer (Otsuka Electronics Co., Ltd, Japan). The morphology of the Ru-1@TPP-PEG-Biotin SAN was analyzed using Energy-Filtering Transmission Electron Microscope (EF-TEM, Carl Zeiss, LIBRA 120, Germany). Fourier transform infrared (FTIR) spectroscopy experiments performed using a JASCO, FT/IR-4200 instrument. The human breast cancer cell line MCF-7 and hepatoma cell line HepG2 were supplied from the Korean Cell Line Bank (KCLB, Korea). Quantum dot conjugation kit (Invitrogen, USA) was used to conjugate the antibody with a quantum dot.

A kit (Quantum Dot Conjugation Kit; Invitrogen, USA) was used to conjugate the antibody with a quantum dot. For the conjugation of the quantum dot to the antibody, antibody carbohydrate domain modification, which is azide attachment to the antibody, and conjugation with the DIBO-modified label were performed. First, the antibodies were treated with dithiothreitol. Subsequently, the antibodies were incubated with the maleimide functionalized quantum dot. Then, the conjugations were treated with 2-mercaptoethanol to remove the maleimide group. The unconjugated quantum dots were filtered using a purification concentrator provided in the kit.