C peptide

Manufactured by R&D Systems

C-peptide is a protein produced by the pancreas alongside insulin. It is released into the bloodstream when insulin is produced and can be measured to evaluate insulin secretion.

Automatically generated - may contain errors

Lab products found in correlation

Igfbp1, by R&D Systems (1 mentions) Igfbp3, by R&D Systems (1 mentions) Mouse c peptide, by ALPCO (1 mentions) Adiponectin, by R&D Systems (1 mentions) Igf 1, by R&D Systems (1 mentions) Leptin, by R&D Systems (1 mentions) Insulin, by Merck Group (1 mentions) Glucagon, by R&D Systems (1 mentions) C peptide, by Merck Group (1 mentions)

2 protocols using c peptide

Mouse and Human Serum Biomarker Analyses

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Mouse whole blood was collected in Eppendorf tubes. It was allowed to coagulate for 2 h at room temperature, centrifuged 20 min at 4,000 rpm and then stored in aliquots in PCR tubes at −80 °C until subsequent use. Whole blood from patients was collected in Vacuette Serum Clot Tubes, centrifuged 20 min at 2,100 rpm and then aliquoted into small tubes and stored at −80 °C until use. All the ELISA assays to detect mouse and human serum level of IGF1, IGFBP1, IGFBP3, C-peptide, leptin and adiponectin were purchased from R&D System except that for mouse C-peptide, which was purchased from Alpco.

Caffa I., Spagnolo V., Vernieri C., Valdemarin F., Becherini P., Wei M., Brandhorst S., Zucal C., Driehuis E., Ferrando L., Piacente F., Tagliafico A., Cilli M., Mastracci L., Vellone V.G., Piazza S., Cremonini A.L., Gradaschi R., Mantero C., Passalacqua M., Ballestrero A., Zoppoli G., Cea M., Arrighi A., Odetti P., Monacelli F., Salvadori G., Cortellino S., Clevers H., De Braud F., Sukkar S.G., Provenzani A., Longo V.D, & Nencioni A. (2020). Fasting-mimicking diet and hormone therapy induce breast cancer regression. Nature, 583(7817), 620-624.

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Plasma Hormone Quantification in Mice

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Mice were fasted overnight or for 4 h, and plasma was collected via tail vein or cardiac puncture. Plasma insulin, C-peptide, and glucagon levels were assessed using insulin (Millipore), C-peptide (ALPCO), or glucagon (R&D systems) ELISAs, respectively, according to the manufacturer's instructions. glucagon levels were measured immediately upon blood collection. insulin levels from perifusion analysis and isolated islets were also measured by ELISA as above.

Roth Flach R.J., Danai L.V., DiStefano M.T., Kelly M., Menendez L.G., Jurczyk A., Sharma R.B., Jung D.Y., Kim J.H., Kim J.K., Bortell R., Alonso L.C, & Czech M.P. (2016). Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia. The Journal of Biological Chemistry, 291(31), 16221-16230.

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