Hemodynamic profile carescape b650

The study was conducted for 2 h. The hemodynamic, gasometric, and ventilation mechanics parameters were assessed (Hemodynamic Profile CARESCAPE B650 (General Electric Company ©, Finland)). Gas exchange was evaluated by measuring the relationship between arterial pressure of oxygen and the fraction of inspired oxygen (PaFiO₂). Arterial oxygen partial pressure (PaO2), arterial carbon dioxide partial pressure (PaCO2), bicarbonate (HCO3), glucose and lactate (ABL 800 Flex Analyzer [Radiometer, Brønshøj, Denmark) were also determined. We used the perfusate returning from the lung for comparison since it would represent the lung microenvironment. Samples of perfusate were taken after 10 min of exposure to fractions of inspired oxygen (FiO₂) at 1.0. Static (Cstat) and dynamic (Cdyn) lung compliance, airway resistance (Raw), peak airway pressure (Ppic), and mean airway pressure (Paw) were measured at the start and end times (Avea™ VIASYSTM Health care, USA); start time refers to the moment each lung was successfully rewarmed to 37 °C [11 , 12 ] at the beginning of EVLP, while end time denotes 120 min of EVLP.

The study was conducted for 4 hours. The hemodynamic, gasometric, and ventilation mechanics parameters were assessed: cardiac output was determined using the thermodilution method (Hemodynamic Profile CARESCAPE B650 (General Electric Company©, Finland)), pulmonary vascular resistance (PVR), partial pressure of oxygen (PaO2), and partial pressure of carbon dioxide (PaCO₂) were measured every hour (ABL 800 Flex Analyzer (Radiometer, Brønshøj, Denmark)); static (Cstat) and dynamic (Cdyn) lung compliance, airway resistance (Raw), and peak inspiratory pressure (PIP) were measured every hour (Avea™ VIASYSTM Healthcare, USA).

All procedures were performed under general anesthesia. Induction was performed with tiletamine zolazepam (4 mg/kg, IM. Zoletil, Virbac, Carros, France) and propofol (4 mg/kg, IV. Recofol, PISA, Guadalajara, JAL, Mexico) and then maintained with isoflurane (Forane, Abbott Mexico S.A. de C.V., Mexico City, Mexico) and fentanyl (0.1 mg/kg, IV. Fentanest, Janssen- Cilag, Puebla, Mexico) as analgesic. The animals were ventilated with pulmonary protection strategies, and the hemodynamic, gasometric, and ventilation mechanics parameters were assessed; the cardiac output was determined using the thermodilution method (Hemodynamic Profile CARESCAPE B650 (General Electric Company ©, Finland)). Recruitment maneuvers are performed to a pulmonary artery wedge pressure (PawP) of 25 cm H₂O. Subsequently, cardiopulmonary block procurement was performed with the technique described by Mariscal, et al. [10 (link)]. All animals were euthanized with an overdose of sodium pentobarbital (150 mg/kg/IV, Anestesal, Pfizer, S.A. de C.V., Guadalajara, Mexico) upon completion of the donor operation.