The procedures of probe making and the FISH experiment were described previously (Inoue et al. 2017b (link)). Morula embryos were obtained by IVF with BDF1 sperm and fixed at 78 hpf. E3.5, E4.0, and E6.5 embryos were obtained by natural mating with BDF1 males. The day of plug was counted as E0.5. E3.5 and E4.0 embryos were flushed out from the uterus at noon of the third day, cultured in KSOM (Millipore), and fixed at noon (E3.5) or midnight (E4.0). For E6.5 embryos, ExE was dissected as described previously (Sugimoto et al. 2015 (link)). For some experiments, embryos were immunostained with rabbit anti-Nanog antibody (1/100; Cosmobio, RCAB002pF) or rabbit anti-H3K27me3 antibody (1/500; Millipore, 07449) after completion of the Xist RNA FISH procedure. The secondary antibodies were Alexa flour 488 donkey anti-rabbit IgG (Life Technologies) and Alexa flour 647 donkey anti-rabbit IgG (Life Technologies).
Alexa flour 647 donkey anti rabbit igg
Manufactured by Thermo Fisher Scientific
Sourced in Hong Kong
Alexa Fluor 647 donkey anti-rabbit IgG is a secondary antibody conjugated with the Alexa Fluor 647 fluorescent dye. It is used to detect and visualize rabbit primary antibodies in immunoassays and other applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alexa flour 647 donkey anti rabbit igg
1
Xist RNA FISH and Immunostaining in Mouse Embryos
2
Collagen I Expression in Tumor Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 48 h of NPs injection and laser irradiation, the tumors were removed and frozen-sectioned for staining with rabbit anti-mouse collagen I antibody (1:200, Abcam, Hong Kong) and Alexa Flour 647 donkey anti-rabbit IgG (Life Technologies, China). Fluorescent images were obtained using CLSM (Ex 649 nm, Em 664 nm) and analyzed using Image-Pro Plus 6.0 software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!