The alpha-glucosidase (from murine intestinal acetone powder (CAS No. I1630)), porcine pancreatic alpha-amylase (CAS No. A3176), dinitrosalicyclic acid (DNS) colour reagent, acarbose (CAS No. 56180-94-0), p-nitrophenyl-d-glucopyranoside (pNPG) (CAS No. 3767-28-0), and soluble starch (CAS No. 9005-84-9) were all products of Sigma–Aldrich Co. (St. Louis, MO, USA). The water used was glass-distilled and obtained from Medical Biochemistry and Pharmacology Laboratory, Kwara State University, Malete, Nigeria. Except otherwise stated, all other reagents and chemicals used are of high analytical grades.
P nitrophenyl d glucopyranoside pnpg
Manufactured by Merck Group
Sourced in United States
P-nitrophenyl-d-glucopyranoside (pNPG) is a synthetic compound commonly used as a substrate in enzymatic assays. It is a colorless and odorless crystalline solid. pNPG is often utilized to measure the activity of glycosidase enzymes, particularly β-galactosidase, through the hydrolysis of the glycosidic bond and the subsequent release of a yellow-colored p-nitrophenol product.
Automatically generated - may contain errors
Lab products found in correlation
Soluble starch, by Merck Group (1 mentions)
Porcine pancreatic alpha amylase, by Merck Group (1 mentions)
Acarbose, by Merck Group (1 mentions)
2 4 6 tripiridyl s triazine, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
α amylase, by Merck Group (1 mentions)
2 2 azino bis, by Merck Group (1 mentions)
α glucosidase, by Merck Group (1 mentions)
3 protocols using p nitrophenyl d glucopyranoside pnpg
1
Alpha-Glucosidase and Amylase Inhibition
2
Amino Acid and Antioxidant Analysis
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Essential and non-essential amino-acid standards came from Sigma–Aldrich Co. (St. Louis, MO, USA). Sigma–Aldrich provided the Folin–Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH),2,4,6-tripiridyl-s-triazine (TPTZ), 2,2′-azino-bis (3-thylbenzthiazoline- 6-sulphonic acid) (ABTS), α-amylase (from Aspergillus oryzae), α -Glucosidase (from Bacillus stearothermophilus), p-nitrophenyl—d -glucopyranoside (PNP-G), bovine serum al-bumin (BSA), sodium azide, and protease (from Bacillus licheniformis). Reagents for the HPLC came from Merck, Darmstadt, Germany.
3
Agroindustrial Waste Valorization for Enzyme Production
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Rice straw, rice hull, rice bran, and brown rice flour were collected from local farmers in Karawang, West Java. Potato Dextrose Agar (PDA), sodium carbonate, CuSO4.5H2O, potassium sodium tartrate, Folin Ciocalteu reagent, Bovine Serum Albumin (BSA), sodium acetate, acetic acid, and p-nitrophenyl--D-glucopyranoside (p-NPG) (Sigma Aldrich), Mandels mineral salts solution (g/L): urea 0.3, (NH4)2SO4 1.4, KH2PO4 2.0, CaCl2.2H2O 0.4, MgSO4.7H2O 0.3, peptone 1.0, Tween 80 0.2, FeSO4.7H2O 0.005, MnSO4.7H2O 0.0016, ZnSO4.7H2O 0.0016, CoCl2.6H2O 0.02.
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