Atomic force microscopy (AFM, MultiMode 8-HR, Bruker, USA) was used to characterize the morphology of the TPAni nanoparticles. To absorb TPAni nanoparticles onto AFM Mica Discs (diameter: 12 mm) (Ted Pella, USA), a 5 μL aliquot of 1 mg/mL TPAni nanoparticles was deposited onto freshly cleaned mica plates for 15 min, which were then washed with DI water and dried under nitrogen. AFM imaging was carried out at 23 °C in contact mode using 127 μm long cantilevers (TESP-V2, BRUKER, USA) with a resonance frequency of 290–310 kHz.
Tesp v2
Manufactured by Bruker
Sourced in United States
The TESP-V2 is a laboratory equipment product from Bruker. It is a tool used for scientific analysis and measurement purposes. The core function of the TESP-V2 is to perform tasks related to the intended application of the equipment. Further details about the specific capabilities and intended use of the TESP-V2 are not available in this response.
Automatically generated - may contain errors
Lab products found in correlation
Multimode 8 hr, by Bruker (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Dimension 3100, by Veeco (1 mentions)
Glass coverslip, by Marienfeld (1 mentions)
Nanoscope iiia multimode afm, by Digital Instruments (1 mentions)
Lambda 750 spectrophotometer, by PerkinElmer (1 mentions)
Dsc 8000 instrument, by PerkinElmer (1 mentions)
Nanoscope 5, by Bruker (1 mentions)
Inspect s50, by Thermo Fisher Scientific (1 mentions)
Provis ax70, by Olympus (1 mentions)
7 protocols using tesp v2
1
AFM Characterization of TPAni Nanoparticles
2
Fabrication of Decane Droplets on Glass
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We prepare decane droplets on glass surfaces by starting with commercially available glass cover slips (Paul Marienfeld GmbH & Co. KG). We clean the glass surfaces in an ultrasonic bath with acetone followed by isopropanol (Sigma-Aldrich Co. LLC.). Samples are blown dry with clean nitrogen. Then decane (anhydrous, ≥99%, Sigma-Aldrich Co. LLC.) is introduced in an ultrasonic nozzle (Atomizer, Sonics & Materials, Inc.). Upon vaporization a fine mist of decane is applied to the glass surface. All AFM measurements have been performed with a Dimension 3100 (Veeco) operated in tapping mode with silicon non-contact tips (TESP-V2, Bruker Nano Inc.). For more details see Supplementary Information .
3
Antibacterial Efficacy of Compound 1 on B. cereus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples containing B. cereus ATCC 14579 (106 CFU mL−1) in MH medium were incubated with compound 1 at various concentrations (0, 8, and 16 µg mL−1) and after 2 h aliquots were taken. A drop (40 µL) containing the bacteria was deposited onto freshly cleaved mica surfaces for 30 min, gently washed with Milli-Q water, and dried under mild nitrogen flux. The surface was examined ex situ, at ambient temperature (21 °C), using Nanoscope IIIa multimode AFM (Digital Instruments, Veeco, Santa Barbara, CA) and etched silicon tips (TESP-V2, Bruker) with a resonance frequency of ca. 300 kHz. Images were acquired with scan rates between 1.2 Hz and 1.5 Hz. It is worth to note that washing and drying steps, as well as tip repetitive scanning did not influence the results presented in this work.
4
Atomic Force Microscopy Analysis of Amyloid-Beta Fibrils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Aβ solution (50 μL) was deposited on clean mica for 5 min and then washed 3 times with 100 μL distilled water (H2O) and gently dried under pure nitrogen gas [17–19 (link)]. AFM images of Aβ fibrils with or without EPPS were acquired using a Multimode V instrument (Veeco, USA) and an Innova (Bruker, USA) operated in tapping mode AFM in the air and at room temperature. To minimize the noise from the air, an AFM housing system was used. A silicon cantilever (TESP-V2, Bruker, USA) was used and scan rate was 0.5 Hz. The resonance frequency of the cantilever for imaging was ∼300 kHz. All AFM imaging areas were 100 μm2. The acquired images were evaluated using NanoScope analysis software 1.8 version. Cross-sectional analysis and calculation of surface roughness were conducted using the same software [7, 17–19 ].
5
Comprehensive Polymer Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
1H NMR spectra were recorded at room temperature on a Bruker-500 spectrometer using deuterated chloroform (CDCl3) as the solvent with tetramethylsilane as an internal standard. The molecular weight of the polymer was characterized by gel permeation chromatography (Waters 1515) with a multi-angle light-scattering detector. THF was used as the eluent. Polarized optical microscopy (POM) was performed on a Zeiss Axio Scope A1 microscope with a Linkam LTSE420 hot stage. UV/Vis absorption spectra were recorded on a PerkinElmer Lambda-750 spectrophotometer. Differential scanning calorimetry (DSC) measurements were carried out on a PerkinElmer DSC 8000 instrument. Atomic force microscopy (AFM) images were obtained on a Bruker Icon instrument in a tapping mode (cantilever model: TESP-V2, Bruker). Contact-angle data were measured by Dataphysics Contact Angle Tester OCA-20. Fluorescent images and readout signal intensity mapping images were obtained using A1R-si Laser Scanning Confocal Microscope equipped with 561 nm laser and 580–620 nm detector. Raman measurements were performed on a DXRxi Micro Raman imaging spectrometer with 780 nm laser.
6
Comprehensive Materials Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fourier transform Infrared Spectroscopy in the attenuated total reflection mode (ATR-FTIR) was performed using a Varian 600 FTIR spectrometer equipped with a ZnSe ATR crystal with a resolution of 4 cm -1 .
Atomic Force Microscopy (AFM) was performed with a Veeco Multimode AFM connected to a Nanoscope V controller was used to image the substrate. AFM measurements were performed in tapping mode in air using a TESP-V2 (Bruker, K = 42 N m -1 ) cantilever.
Contact angles were measured using a Ramé-Hart contact angle system (Model 290). The average contact angle value was obtained by dispensing 1 µL-droplets of Milli-Q water at five different positions on the film-modified substrates. Cyclic voltammetry (CV) was performed using a Gamry REF600 potentiostat. Gold electrodes were prepared by sputtering on glass substrates employing a thin layer of Ti to improve the adhesion. The electrochemical cell was a three electrodes Teflon cell with 2 ml volume capacity. The Au working electrodes sealed with an o-ring defining an electroactive area of 0.145 cm2. The counter electrode was a Pt wire and a Ag/AgCl (3 M NaCl) electrode was employed as reference.
Atomic Force Microscopy (AFM) was performed with a Veeco Multimode AFM connected to a Nanoscope V controller was used to image the substrate. AFM measurements were performed in tapping mode in air using a TESP-V2 (Bruker, K = 42 N m -1 ) cantilever.
Contact angles were measured using a Ramé-Hart contact angle system (Model 290). The average contact angle value was obtained by dispensing 1 µL-droplets of Milli-Q water at five different positions on the film-modified substrates. Cyclic voltammetry (CV) was performed using a Gamry REF600 potentiostat. Gold electrodes were prepared by sputtering on glass substrates employing a thin layer of Ti to improve the adhesion. The electrochemical cell was a three electrodes Teflon cell with 2 ml volume capacity. The Au working electrodes sealed with an o-ring defining an electroactive area of 0.145 cm2. The counter electrode was a Pt wire and a Ag/AgCl (3 M NaCl) electrode was employed as reference.
7
Bacterial Biofilm Quantification on Plastics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bacteria were enumerated on 70 plastic pieces sampled at 22 stations (from 1 to 4 pieces per manta, when microscopic observation was possible) by epifluorescence microscopy using an Olympus AX-70 PROVIS after 4', 6-diamidino-2-phenylindole (DAPI) staining. The covering of the surface area of the plastic by bacterial cells was determined using Image J software. Cell enrichment factors were calculated by the ratio between bacterial counts in PMD and in the FL fraction, brought forward to a same volume of 1 mm -3 when considering a mean PMD thickness of 100 µm.
For qualitative assessment of biofilm structure, a random collection of 8 samples was chosen for scanning electron microscopy (SEM) using Inspect S50 (FEI, Hillsboro, OR, USA), as described previously (Zettler et al., 2013) . Some of the samples were also analyzed by atomic force microscope (AFM) to get more resolved insight of the surface, using a Nanoscope V in dynamic mode (Bruker instruments, Madisson, WI, USA) and standard silicon probes (Bruker, TESP-V2) (Binnig and Quate, 1986) .
For qualitative assessment of biofilm structure, a random collection of 8 samples was chosen for scanning electron microscopy (SEM) using Inspect S50 (FEI, Hillsboro, OR, USA), as described previously (Zettler et al., 2013) . Some of the samples were also analyzed by atomic force microscope (AFM) to get more resolved insight of the surface, using a Nanoscope V in dynamic mode (Bruker instruments, Madisson, WI, USA) and standard silicon probes (Bruker, TESP-V2) (Binnig and Quate, 1986) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!