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Alexa fluor 488 epcam

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Alexa Fluor 488 EpCAM is a fluorescently labeled antibody that binds to the epithelial cell adhesion molecule (EpCAM) protein. EpCAM is commonly expressed on the surface of epithelial cells and is often used as a marker for identifying and isolating these cells.

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Lab products found in correlation

S3 cell sorter, by Bio-Rad (2 mentions) Qdot 800 cd45, by Thermo Fisher Scientific (2 mentions)

2 protocols using alexa fluor 488 epcam

1

Isolation and Characterization of CTCs

Cited 1 time
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In a subset of patients (n = 5), whole peripheral and portal venous blood samples were collected into CPT tubes and centrifuged for 30 minutes at 1500g at room temperature as previously described.16 (link) Briefly, the mononuclear layer cells were isolated according to the manufacturer’s instructions and washed twice with phosphate-buffered saline. Cells then were stained with Alexa Fluor 488 EpCAM at 5 uL per million cells (cat: 324210; Biolegend) and CD45 antibody at 1 uL per million cells (cat: Q10156, QDot 800 CD45; Invitrogen) for 30 minutes on ice. AGS cells (human gastric cancer cell line obtained from ATCC, Manassas, VA) were prepared as an EpCAM+ control, and unstained AGS cells served as negative controls. Cell sorting was performed using standard procedure either by BD FACSARIA III or a Bio-Rad S3 cell sorter. With flow cytometry CTC isolation, CD45−, EpCAM+, and DAPI+ cells were collected as CTC cells for further genomic and proteomic analyses.
Catenacci D.V., Chapman C.G., Xu P., Koons A., Konda V.J., Siddiqui U.D, & Waxman I. (2015). Acquisition of Portal Venous Circulating Tumor Cells From Patients With Pancreaticobiliary Cancers by Endoscopic Ultrasound. Gastroenterology, 149(7), 1794-1803.e4.
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2

Isolation and Characterization of CTCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
In a subset of patients (n = 5), whole peripheral and portal venous blood samples were collected into CPT tubes and centrifuged for 30 minutes at 1500g at room temperature as previously described.16 (link) Briefly, the mononuclear layer cells were isolated according to the manufacturer’s instructions and washed twice with phosphate-buffered saline. Cells then were stained with Alexa Fluor 488 EpCAM at 5 uL per million cells (cat: 324210; Biolegend) and CD45 antibody at 1 uL per million cells (cat: Q10156, QDot 800 CD45; Invitrogen) for 30 minutes on ice. AGS cells (human gastric cancer cell line obtained from ATCC, Manassas, VA) were prepared as an EpCAM+ control, and unstained AGS cells served as negative controls. Cell sorting was performed using standard procedure either by BD FACSARIA III or a Bio-Rad S3 cell sorter. With flow cytometry CTC isolation, CD45−, EpCAM+, and DAPI+ cells were collected as CTC cells for further genomic and proteomic analyses.
Catenacci D.V., Chapman C.G., Xu P., Koons A., Konda V.J., Siddiqui U.D, & Waxman I. (2015). Acquisition of Portal Venous Circulating Tumor Cells From Patients With Pancreaticobiliary Cancers by Endoscopic Ultrasound. Gastroenterology, 149(7), 1794-1803.e4.
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