2695 separation module

Gel permeation chromatography (GPC) was carried out on a Waters (Millford, MA) chromatograph equipped with a Waters Alliance high pressure liquid chromatography (HPLC) system pump (2695 Separation Module) and four Visco Gel I-Series columns from Viscotek (dimensions = 7.8 mm × 30 cm). Detection was provided by a Waters 2414 differential refractometer and N,N-dimethyl formamide (DMF) with 0.1 % LiBr used as the mobile phase. Copolymer chromatograms were run at room temperature and calibrated to poly(methyl methacrylate) (PMMA) standards. Dynamic light scattering (DLS) was performed on a Wyatt Technology (Goleta, CA) DynaPro NanoStar™ at room temperature. Data was collected on 0.1 wt% aqueous nanoparticle solutions filtered through a 0.2 μm filter. Zeta potential measurements were acquired on a Malvern Zetasizer (Zetasizer Nano ZS ZEN3600, Westborough, MA). A Bioscan 200 imaging scanner (Bioscan, Washington, DC) was used to read the instant thin layer chromatography (ITLC) plates (Pall ITLC-SG plates, VWR International, Batavia, IL). Fast protein liquid chromatography (FPLC) and radio-FPLC were performed using an ÄKTA FPLC system (GE Healthcare Biosciences, Pittsburgh, PA) equipped with a Beckman 170 Radioisotope Detector (Beckman Instruments, Fullerton, CA).

Gel permeation chromatography (GPC) was carried out on a Waters (Millford, MA) chromatograph equipped with a Waters Alliance high pressure liquid chromatography (HPLC) system pump (2695 Separation Module) and four Visco Gel I-Series columns from Viscotek (dimensions = 7.8 mm × 30 cm). Detection was provided by a Waters 2414 differential refractometer and dimethyl formamide with 0.1 % LiBr was used as the mobile phase. Copolymer chromatograms were run at room temperature and calibrated to poly(methyl methacrylate) (PMMA) standards. Dynamic light scattering (DLS) was performed on a Wyatt Technology (Goleta, CA) DynaPro NanoStar™ at room temperature. Data was collected on 0.1 wt% aqueous nanoparticle solutions filtered through a 0.2 μm filter. Zeta potential measurements were acquired on a Malvern Zetasizer (Zetasizer Nano ZS ZEN3600). A Bioscan 200 imaging scanner (Bioscan, Washington, DC) was used to read the instant thin layer chromatography (ITLC) plates (Pall ITLC-SG plates, VWR International, Batavia, IL). Fast protein liquid chromatography (FPLC) and radio-FPLC were performed using an ÄKTA FPLC system (GE Healthcare Biosciences) equipped with a Beckman 170 Radioisotope Detector (Beckman Instruments, Fullerton, CA). All other instrumentation can be found in our previous report (31 (link)).