The following ELISA-based assays were purchased: Estradiol (VWRCat. No. 102966-728), Free Testosterone (VWR Cat. No. 75871-402), Total Testosterone (VWR Cat. No. 75871-120) Insulin (EMD Millipore Cat. No. EZRMI-13K), Glucagon (FisherScientific Cat. No. 50-104-3269), Cortisol (FisherScientific Cat. No. EIAHCOR), LH (FisherScientific Cat. No. EHLH), and FSH (FisherScientific Cat. No. 50-148-7181). Serum samples randomly chosen from 6 male controls, 11 methyl-diet males, 7 female controls, and 17 methyl-diet females were tested in each ELISA assay. Serum samples were loaded into 96 well plates containing antibodies specific to the hormone of interest. Wells were washed following incubation, and a secondary antibody with a conjugated reporter was added. Wells were washed again after incubation with the secondary antibody, and a substrate solution was added for 10 minutes. The reactions were stopped and the assays were analyzed by a spectrophotometric plate reader at 450nm. Two tailed t-tests were used to determine if there were significant changes in hormones between male controls and methyl-diet males as well as between control females and methyl-diet females.
Cortisol
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cortisol is a laboratory measurement used to assess adrenal gland function. It is a hormone produced by the adrenal glands and plays a crucial role in the body's response to stress. The measurement of cortisol levels can provide valuable information about the body's ability to regulate and respond to stress.
Automatically generated - may contain errors
2 protocols using cortisol
1
Hormonal Changes in Methyl-Diet Individuals
2
Biomarker Profiling in Plasma
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Ghrelin (#BMS2192, Thermo Fisher, MA, USA), insulin (ab100578, Abcam, Berlin, Germany), irisin (EK-067-29, Phoenix Pharmaceuticals, Burlingame, CA, USA), glucagon (#EHGCG, Thermo Fisher, MA, USA), cortisol (#15642299, Fisher Scientific, Reinach, Switzerland), neopterin (#RE59321, IBL International, Hamburg, Germany), and total antioxidative capacity (TAC, measurement of the combination of both small molecule antioxidants and proteins by Cu2+ reduction) (ab65329 Abcam, Berlin, Germany) were analyzed in the plasma fraction using respective enzyme-linked immunosorbent assays (ELISA) according to the manufactures’ instruction.
Plasma kynurenine concentrations were determined by spectrometry using an established protocol [27 (link)]. Briefly, serum samples were deproteinized with acetic acid trichloride and following deproteinization. Kynurenine reacts under the use of 4-dimethylamino-benzaldehyde into a yellow product, which can be measured spectrometrically at 492 nm and quantified with concomitant preparation of a stand with known concentrations.
Tissue glucose was measured with an intracutaneous glucose sensor (Free style libre 3, analyzed by App-based program LibreView, Abbot Diabetes Care, Alameda, CA, USA).
Plasma kynurenine concentrations were determined by spectrometry using an established protocol [27 (link)]. Briefly, serum samples were deproteinized with acetic acid trichloride and following deproteinization. Kynurenine reacts under the use of 4-dimethylamino-benzaldehyde into a yellow product, which can be measured spectrometrically at 492 nm and quantified with concomitant preparation of a stand with known concentrations.
Tissue glucose was measured with an intracutaneous glucose sensor (Free style libre 3, analyzed by App-based program LibreView, Abbot Diabetes Care, Alameda, CA, USA).
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