2 2 azobis 2 aminopropane dihydrochloride 7 mm

The 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS), Sigma-Aldrich, Co.) radical-scavenging activity was measured using a slight modification of the method reported by Proestos [19 (link)]. 2,2′-azobis (2-aminopropane) dihydrochloride (7 mM) (Sigma-Aldrich, Co.) was mixed with 2.45 mM ABTS and then reacted for 16 h at 4 °C. A 50 μL quantity of the sample and 100 μL of the ABTS solution were reacted at 23 °C for 20 min after adding them to a 96-well plate. The absorbance was measured at 734 nm; ascorbic acid (100 μg/mL) was used as the positive control.

The ABTS (Sigma-Aldrich. Co) radical scavenging activity was measured using a previously reported method by Proestos with slight modification [41 (link)]. 2,2′-azobis (2-aminopropane) dihydrochloride (7 mM) (Sigma-Aldrich, Co., St. Louis, MO, USA) was mixed with 2.45 mM ABTS and allowed to react for 16 h at 4 °C. Typically, 50 μL of the sample and 100 μL of the ABTS solution were mixed and allowed to react at 23 °C for 20 min after addition to a 96 well plate. The absorbance was measured at 734 nm using a microplate spectrophotometer (Epoch, Biotek Instruments, Inc., VT, USA). Ascorbic acid (100 μg/mL) was used as a positive control.