Ssttm2.1 cre zjh j

Manufactured by Jackson ImmunoResearch

The Ssttm2.1(cre)Zjh/J is a genetically modified mouse strain that expresses the Cre recombinase enzyme under the control of the Somatostatin 2 promoter. This mouse line can be used for studies involving the targeted manipulation of gene expression in somatostatin-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

Pvalbtm1 cre arbr j, by Jackson ImmunoResearch (3 mentions) B6 cg gt rosa 26sortm14 cag tdtomato hze j, by Jackson ImmunoResearch (2 mentions) Viptm1 cre zjh j, by Jackson ImmunoResearch (1 mentions) B6 cg tg drd1a tdtomato 6calak j, by Jackson ImmunoResearch (1 mentions) Slc32a1tm2 cre lowl j, by Jackson ImmunoResearch (1 mentions) 26sortm9, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions)

9 protocols using ssttm2.1 cre zjh j

Imaging Awake Transgenic Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experimental procedures were conducted according to the UK Animals Scientific Procedures Act (1986). Experiments were performed at University of Sussex under personal and project licenses granted by the Home Office following review by the Animal Welfare and Ethics Review Board.
Experiments were performed on 27 adult transgenic mice of either sex (4–10 months old) expressing the Cre recombinase in specific subsets of interneurons on a C57BL/6J background. Results are reported from nine VIP-Cre mice (VIP tm1(cre)Zjh/J Jackson #010908), eight PV-Cre (Pvalb tm1(cre)Arbr/J, Jackson #008069), and ten SST-Cre (SST tm2.1(cre)Zjh/J, Jackson #013044). Mice were housed individually on inverted light-dark cycles and had access to a complex enriched environment after the end of each imaging session. This environment was large (~80 × 40 × 40 cm) and contained a number of toys, platforms and tubes that encouraged motor activity. As a result, mice were engaged in running the large majority of the time during an imaging experiment.

Heintz T.G., Hinojosa A.J., Dominiak S.E, & Lagnado L. (2022). Opposite forms of adaptation in mouse visual cortex are controlled by distinct inhibitory microcircuits. Nature Communications, 13, 1031.

+ Open protocol

+ Expand

Cre-Dependent Labeling of Somatostatin Neurons

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

SST-Ires-Cre mice (Ssttm2.1(cre)Zjh/J, Jackson Lab strain # 013044, [5] were crossed with Rosa-tdTomato reporter (B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Jackson lab Strain # 007905, [18] (link)or GCAMP33 reporter (B6;129S-Gt(Rosa)26Sor/J Jackson lab Strain # 014538, mice, [17] (link). The resulting offspring displayed the Rosa-tdTomato or the GCAMP3 proteins, respectively, in the Cre-expressing neurons. Genotyping was performed with a commercial vendor (Transnetyx, Cordova, TN).

Grosser S., Queenan B.N., Lalchandani R.R, & Vicini S. (2014). Hilar Somatostatin Interneurons Contribute to Synchronized GABA Activity in an In Vitro Epilepsy Model. PLoS ONE, 9(1), e86250.

+ Open protocol

+ Expand

Interneuron Labeling Protocols for Imaging and Behavior

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments were approved by the Washington University Animal Care and Use Committee. Heterozygotes (+/–) from two cre-driver mice lines on a C57Bl/6J genetic background were used to label parvalbumin-expressing and somatostatin-expressing inhibitory interneurons: SST^{tm2.1(cre)Zjh}/J (SOM-cre) and Pvalb^tm1(cre)Arbr/J (PV-cre; Jackson Labs). All imaging data were from SOM-ints while behavioral data in Figure 1 were from PV- and SOM-ints.

Arriaga M, & Han E.B. (2019). Structured inhibitory activity dynamics in new virtual environments. eLife, 8, e47611.

+ Open protocol

+ Expand

Optogenetic Activation of Somatostatin Interneurons in Mouse Dentate Gyrus

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant adeno-associated viruses (rAAVs) encoding Channelrhodopsin-2 (Chr2) and tdTomato were injected into the ventral dentate gyrus (relative to bregma: x, 2.5 mm; y, 2.9 mm; z, 2.3, 2.6, 2.9 mm) of SOM-Cre mice (Sst^{tm2.1(cre)Zjh}/J, Jackson Laboratory stock: 013044)³⁹. The expression cassette of the rAAV contained tdTomato and Chr2 between two inverted loxP sites (rAAV1.CAGGS. Flex.Chr2. tdTomato.WPRES.SV40; Addgene catalog #18917). Slices were prepared >14 d after injection. Full-field illumination (488 nm, 20 ms; pE-100; CoolLED) was applied 5 ms before extracellular stimulation of the perforant path at an intensity just sufficient to evoke action potentials. Twenty to sixty trials with and without light-mediated SOMI-activation were used for calculating the probability of action potential generation.

Elgueta C, & Bartos M. (2019). Dendritic inhibition differentially regulates excitability of dentate gyrus parvalbumin-expressing interneurons and granule cells. Nature Communications, 10, 5561.

+ Open protocol

+ Expand

Validating Viral Transduction in Sst-Expressing Mice

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Viral transduction was validated in C57BL/6J Sst^Gfp/+ mice (Taniguchi et al., 2011 (link); Lin and Sibille, 2015 (link)). Behavioral and molecular experiments were performed using C57BL/6J Sst^Cre/+ mice (Sst^{tm2.1(cre)Zjh}/J, Jackson Laboratories, Bar Harbour, ME; #913944), postnatal day zero at surgery and 9-16 weeks at testing. Mice were maintained under a 12-hour-light/-dark cycle (7 am-7 pm) with ad libitum food and water and were group housed (4/cage) except during behavioral testing (1/cage). Tests were performed according to Institutional and Canadian Council on Animal Care guidelines.

Fee C., Prevot T.D., Misquitta K., Knutson D.E., Li G., Mondal P., Cook J.M., Banasr M, & Sibille E. (2021). Behavioral Deficits Induced by Somatostatin-Positive GABA Neuron Silencing Are Rescued by Alpha 5 GABA-A Receptor Potentiation. International Journal of Neuropsychopharmacology, 24(6), 505-518.

+ Open protocol

+ Expand

Genetically Modified Mouse Models

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments were conducted in accordance with the Nagoya University Regulations on Animal Care and Use in Research and were approved by the Institutional Animal Care and Use Committee, Nagoya University (approval number R210154). Mice were group-housed after weaning and kept under 12-h light/dark cycle with food and water provided ad libitum.
Prkcd-cre mice {Tg(Prkcd-glc-1/CFP,-cre)EH124Gsat; stock #011559-UCD} were obtained from the Mutant Mouse Resource and Research Center. Sst (somatostatin)-cre mice {Sst tm2.1(cre)Zjh/J; stock #013044} and Ai14 mice {B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze/J; stock #007914} were obtained from the Jackson Laboratory. Crh (corticotropin-releasing hormone)-cre mice {Crh} were described in the previous study (Itoi et al., 2014 (link)). Wild-type C57BL/6J mice were purchased from SLC Japan (Shizuoka, Japan).

Ueda S., Hosokawa M., Arikawa K., Takahashi K., Fujiwara M., Kakita M., Fukada T., Koyama H., Horigane S.I., Itoi K., Kakeyama M., Matsunaga H., Takeyama H., Bito H, & Takemoto-Kimura S. (2021). Distinctive Regulation of Emotional Behaviors and Fear-Related Gene Expression Responses in Two Extended Amygdala Subnuclei With Similar Molecular Profiles. Frontiers in Molecular Neuroscience, 14, 741895.

+ Open protocol

+ Expand

Dual-labeled Somatostatin-expressing Neurons

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following mouse lines were used in this study:
SOM-Cre: Ssttm2.1(cre)Zjh/J [The Jackson Laboratory, stock number 013044]; ROSA-tdTomato reporter: B6.CG.Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J [The Jackson Laboratory, stock number 007914]; Drd1a-tdTomato/Drd2-GFP reporter: The two reporter lines, B6.Cg-Tg(Drd1a-tdTomato)6Calak/J [The Jackson Laboratory, stock number 016204; (Ade et al., 2011 (link))] and Tg(Drd2-EGFP)S118Gsat/Mmnc [MMRRC, stock number 000230-UNC] were crossed to generate a reporter for both D1- and D2-expressing neurons. A male mouse positive for both Drd1a-tdTomato/Drd2-EGFP was provided to us by Dr. Carlos Paladini at UTSA.
SOM-Cre female mice were crossed with a ROSA-tdTomato reporter male mouse to generate a SOM-Cre-tdTomato line (somatostatin-containing neurons expressed both Cre and tdTomato). SOM-Cre female mice were crossed with a Drd1a-tdTomato/Drd2-GFP reporter male mouse to generate a SOM-Cre-D1/D2 line (somatostatin-containing neurons expressed Cre; D1 neurons expressed tdTomato; D2 neurons expressed GFP).

Rock C., Zurita H., Wilson C, & Apicella A.J. (2016). An inhibitory corticostriatal pathway. eLife, 5, e15890.

+ Open protocol

+ Expand

Transgenic Mouse Strains for Neuroscience

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were 6–12 weeks old at the time of experiments. Mice were acquired from Jackson Laboratories: Slc32a1^tm2(cre)Lowl/J (VGAT-Cre); Pvalb^tm1(cre)Arbr/J (PV-Cre); Sst^{tm2.1(cre)Zjh}/J (SOM-Cre); Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J (Ai9); C57BL/6J; CBA/J; BALB/cJ. Both female and male animals were used and housed at 21 °C and 40% humidity with a reverse light cycle (12–12 h). All experiments were performed during their dark cycle. All procedures were approved and conducted in accordance with the Institutional Animal Care and Use Committee at the University of North Carolina at Chapel Hill and Osaka University as well as guidelines of the National Institutes of Health.

Kline A.M., Aponte D.A., Tsukano H., Giovannucci A, & Kato H.K. (2021). Inhibitory gating of coincidence-dependent sensory binding in secondary auditory cortex. Nature Communications, 12, 4610.

+ Open protocol

+ Expand

Validating Viral Transduction in Sst Gfp/+ Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Viral transduction was validated in C57BL/6J Sst Gfp/+ mice (Taniguchi et al., 2011; Lin and Sibille, 2015) . Behavioral and molecular experiments were performed using C57BL/6J Sst Cre/+ mice (Sst tm2.1(cre)Zjh /J, Jackson Laboratories, ME; #913944), postnatal day zero (PND0) at surgery and 9-16W at testing. Mice were maintained under 12-hour light/dark cycle (7am-7pm) with food and water ad libitum, group-housed (4/cage), except during behavioral testing (1/cage). Tests were performed according to Institutional and Canadian Council on Animal Care (CCAC) guidelines.

Fee C., Prévôt T.D., Misquitta K.A., Knutson D.E., Li G., Mondal P., Cook J.M., Banasr M., & Sibille E. (2020). Depressive-like behaviors induced by somatostatin-positive GABA neuron silencing are rescued by alpha 5 GABA-A receptor potentiation.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!