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Milliplex mouse cytokine chemokine 32 plex kit

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The MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit is a multiplex assay designed for the simultaneous quantitative measurement of 32 different mouse cytokines and chemokines in a single sample. The kit utilizes Luminex technology to enable the detection and analysis of multiple analytes in a single well.

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Lab products found in correlation

Milliplex mouse cytokine chemokine 13 plex kit, by Merck Group (5 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Bicinchoninic acid protein assay, by Thermo Fisher Scientific (1 mentions) Tissuelyser 2, by Qiagen (1 mentions)

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Milliplex Mouse Cytokine/Chemokine kit Mouse Cytokine 32-plex kit Mouse Milliplex kit Milliplex kit

11 protocols using milliplex mouse cytokine chemokine 32 plex kit

1

Multiplexed Cytokine Profiling of Mouse TILs

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In this study, we used Luminex xMAP technology for multiplexed quantification of 45 Mouse cytokines, chemokines, and growth factors. The multiplexing analysis was performed using the Luminex 100 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, Alberta). Forty-four markers were simultaneously measured in the supernatants from gp100 re-stimulated TILs using a MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit and a MILLIPLEX Mouse Cytokine/Chemokine 13-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol. The 45-plex consisted of Eotaxin, Erythropoietin, 6Ckine, Fractalkine, G-CSF, GM-CSF, IFNB1, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-16, IL-17, IL-20, IP-10, KC, LIF, LIX, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-2, MIP-3α, MIP-3B, RANTES, TARC, TIMP-1, TNFα, and VEGF. The assay sensitivities of these markers range from 0.3 to30.6 pg/mL for the 45-plex. Individual analyte values are available in the MILLIPLEX protocol.
Vaena S., Chakraborty P., Lee H.G., Janneh A.H., Kassir M.F., Beeson G., Hedley Z., Yalcinkaya A., Sofi M.H., Li H., Husby M.L., Stahelin R.V., Yu X.Z., Mehrotra S, & Ogretmen B. (2021). Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell response. Cell reports, 35(5), 109076.
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2

Multiplex Cytokine Profiling in Mice

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At euthanasia, blood was obtained by cardiac puncture, coagulated for 30 min at room temperature, and centrifuged at 3000 rpm for 10 min at 4 °C to collect serum. Serum (n = 5–6 mice per diet/per timepoint) was diluted 2-fold in DPBS and analyzed using the MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit (Millipore, St. Charles, MO, USA) on the Luminex™ 200 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, Alberta). The multiplex assay quantified Eotaxin, G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα, and VEGF. The assay sensitivities range from 0.3 to 30.6 pg/mL.
Kerr G.J., To B., White I., Millecamps M., Beier F., Grol M.W., Stone L.S, & Séguin C.A. (2021). Diet-induced obesity leads to behavioral indicators of pain preceding structural joint damage in wild-type mice. Arthritis Research & Therapy, 23, 93.
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3

Multiplex Cytokine/Chemokine Quantification

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Cytokines, chemokines, and growth factors were analyzed by Eve Technologies Corp. (Calgary, AB, Canada), with a Milliplex Mouse Cytokine/Chemokine 32-plex kit (Millipore, MO), according to the manufacturer’s protocol, on a Luminex 100 system (Luminex, Austin, TX). Tissue specimens (10–25 mg) were homogenized in 200 μl of 20 mM Tris-HCl (pH 7.5) buffer with 0.5% Tween 20, 150 mM NaCl, and protease inhibitors and centrifuged for 10 min at 4 °C, and the supernatant was transferred to a fresh tube. Protein content was measured using the bicinchoninic acid protein assay (Thermo Fisher Scientific; IL). For supernatant analysis, conditioned media were centrifuged before multiplex analysis. Measurements were normalized to the cell protein. Plasma samples were diluted with an equal volume of PBS before analysis.
Tang X., Wang X., Zhao Y.Y., Curtis J.M, & Brindley D.N. (2017). Doxycycline attenuates breast cancer related inflammation by decreasing plasma lysophosphatidate concentrations and inhibiting NF-κB activation. Molecular Cancer, 16, 36.
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4

Comprehensive Cytokine Profiling in Adar Mice

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Blood cytokine levels in Adar+/+ and AdarP195A/− mice were measured by Luminex assay. Forty-five (45) cytokines and chemokines were measured utilizing Luminex xMAP technology. The multiplexing analysis was performed using the Luminex 200 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, Alberta, Canada). Forty-five markers were simultaneously measured in the samples using a MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit and a MILLIPLEX Mouse Cytokine/Chemokine 13-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol. The 45-plex consisted of Eotaxin, Erythropoietin, 6Ckine, Fractalkine, G-CSF, GM-CSF, IFNB1, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-16, IL-17, IL-20, IP-10, KC, LIF, LIX, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-2, MIP-3α, MIP-3B, RANTES, TARC, TIMP-1, TNFα, and VEGF. The assay sensitivities of these markers range from 0.3–30.6 pg/mL.
Lomans B.P., Op den Camp H.J., Pol A., van der Drift C, & Vogels G.D. (1999). Role of Methanogens and Other Bacteria in Degradation of Dimethyl Sulfide and Methanethiol in Anoxic Freshwater Sediments. Applied and Environmental Microbiology, 65(5), 2116-2121.
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5

Comprehensive Cytokine Profiling in BAL

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Cytokine levels in BAL fluid were measured by Eve Technologies Corporation (Calgary, Alberta, Canada) using a MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit (Millipore, St. Charles, MO) according to the manufacturer's protocol. The 32-plex consisted of eotaxin, granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-gamma, interleukin (IL)-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL- 6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IFN gamma-induced protein (IP)-10, keratinocyte chemoattractant (KC), leukemia inhibitory factor (LIF), CXC motif ligand (LIX), monocyte chemoattractant protein (MCP)-1, macrophage (M)-CSF, monokine induced by gamma interferon (MIG), macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, MIP-2, chemokine C-C motif ligand 5 (CCL5/RANTES), tumor necrosis favotor (TNF)-alpha, and vascular endothelial growth factor (VEGF). The sensitivities of the assay to these markers ranged from 0.3 to 63.6 pg/mL.
Pirela S.V., Lu X., Miousse I., Sisler J.D., Qian Y., Guo N., Koturbash I., Castranova V., Thomas T., Godleski J, & Demokritou P. (2016). Effects of intratracheally instilled laser printer-emitted engineered nanoparticles in a mouse model: A case study of toxicological implications from nanomaterials released during consumer use. NanoImpact, 1, 1-8.
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6

Multiplexed Cytokine Profiling of Mouse TILs

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In this study, we used Luminex xMAP technology for multiplexed quantification of 45 Mouse cytokines, chemokines, and growth factors. The multiplexing analysis was performed using the Luminex 100 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, Alberta). Forty-four markers were simultaneously measured in the supernatants from gp100 re-stimulated TILs using a MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit and a MILLIPLEX Mouse Cytokine/Chemokine 13-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol. The 45-plex consisted of Eotaxin, Erythropoietin, 6Ckine, Fractalkine, G-CSF, GM-CSF, IFNB1, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-16, IL-17, IL-20, IP-10, KC, LIF, LIX, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-2, MIP-3α, MIP-3B, RANTES, TARC, TIMP-1, TNFα, and VEGF. The assay sensitivities of these markers range from 0.3 to30.6 pg/mL for the 45-plex. Individual analyte values are available in the MILLIPLEX protocol.
Vaena S., Chakraborty P., Lee H.G., Janneh A.H., Kassir M.F., Beeson G., Hedley Z., Yalcinkaya A., Sofi M.H., Li H., Husby M.L., Stahelin R.V., Yu X.Z., Mehrotra S, & Ogretmen B. (2021). Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell response. Cell reports, 35(5), 109076.
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7

Multiplex Cytokine Profiling of Tumor Tissue

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Thirty-two cytokines, chemokines and growth factors were measured in plasma and primary tumor tissue by Eve Technologies Corp. (Calgary, AB, Canada), using a Milliplex Mouse Cytokine/Chemokine 32-plex kit (Millipore, St. Louis, MO, USA), according to the manufacturer’s protocol, on a Luminex 100 system (Luminex, Austin, TX, USA). Tissue specimens (25–30 mg) were homogenized using TissueLyser II (Qiagen, Venlo, The Netherlands) in 200 μL of 20 mM Tris-HCl (pH 7.5) buffer with 0.5% Tween 20, 150 mM NaCl and protease inhibitor cocktail (HB9081, Hello Bio, Princeton, NJ, USA) and centrifuged for 10 min at 4 °C. The supernatant was transferred to a fresh tube, and the protein content was measured using the Pierce BCA protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA). Samples were normalized to equal protein concentrations before analyses. Plasma samples were diluted with the same volume of PBS before measurements.
Shinde A., Tang X., Singh R, & Brindley D.N. (2023). Infliximab, a Monoclonal Antibody against TNF-α, Inhibits NF-κB Activation, Autotaxin Expression and Breast Cancer Metastasis to Lungs. Cancers, 16(1), 52.
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8

Cytokine/Chemokine Profiling of EMT6 Cells

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Supernatants were harvested from EMT6 cells infected with live or UV-inactivated RV (DV) for 24 h in 6-well plates. The multiplexing analysis was performed using the Luminex™ 100 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, AB, Canada). Thirty-two markers were simultaneously measured in the supernatant using a MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit (Millipore, St. Louis, MO, USA) according to the manufacturer’s protocol. Assay sensitivities for these analytes ranged from 0.2 to 63.6 pg/mL.
Mostafa A.A., Meyers D.E., Thirukkumaran C.M., Liu P.J., Gratton K., Spurrell J., Shi Q., Thakur S, & Morris D.G. (2018). Oncolytic Reovirus and Immune Checkpoint Inhibition as a Novel Immunotherapeutic Strategy for Breast Cancer. Cancers, 10(6), 205.
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9

Comprehensive Cytokine Profiling in Mice

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Forty-five [45 (link)] cytokines and chemokines were measured utilizing Luminex® xMAP® technology. The multiplexing analysis was performed using the Luminex™ 200 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, Alberta, Canada). Forty-five markers were simultaneously measured in the samples using a MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit and a MILLIPLEX Mouse Cytokine/Chemokine 13-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer’s protocol. The 45-plex consisted of Eotaxin, Erythropoietin, 6Ckine, Fractalkine, G-CSF, GM-CSF, IFNB1, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-16, IL-17, IL-20, IP-10, KC, LIF, LIX, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-2, MIP-3α, MIP-3B, RANTES, TARC, TIMP-1, TNFα, and VEGF. The assay sensitivities of these markers range from 0.3 to 30.6 pg/mL.
Guo X., Wiley C.A., Steinman R.A., Sheng Y., Ji B., Wang J., Zhang L., Wang T., Zenatai M., Billiar T.R, & Wang Q. (2021). Aicardi-Goutières syndrome-associated mutation at ADAR1 gene locus activates innate immune response in mouse brain. Journal of Neuroinflammation, 18, 169.
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10

Comprehensive Cytokine and Chemokine Profiling

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Forty-ve (45) cytokines and chemokines were measured utilizing Luminex® xMAP® technology. The multiplexing analysis was performed using the Luminex™ 200 system (Luminex, Austin, TX, USA) by Eve Technologies Corp. (Calgary, Alberta, Canada). Forty-ve markers were simultaneously measured in the samples using a MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit and a MILLIPLEX Mouse Cytokine/Chemokine 13-plex kit (Millipore, St. Charles, MO, USA) according to the manufacturer's protocol. The 45-plex consisted of Eotaxin, Erythropoietin, 6Ckine, Fractalkine, G-CSF, GM-CSF, IFNB1, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-16, IL-17, IL-20, IP-10, KC, LIF, LIX, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-2, MIP-3α, MIP-3B, RANTES, TARC, TIMP-1, TNFα, and VEGF. The assay sensitivities of these markers range from 0.3 -30.6 pg/mL.
Guo X., Wiley C.A., Steinman R.A., Sheng Y., Ji B., Wang J., Wang T., Zenatai M., Zhang L., & Wang Q. (2021). Aicardi-Goutières syndrome associated mutation at ADAR1 gene locus activates innate immune response in mouse brain.
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