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Peg sh

Manufactured by JenKem Technology
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PEG-SH is a polyethylene glycol-based thiol group-containing compound. It is a water-soluble and biocompatible molecule that can be used for various applications in biotechnology and materials science.

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Lab products found in correlation

Peg mal, by JenKem Technology (2 mentions) N cadherin, by Sangon (1 mentions) Calcium carbonate, by Merck Group (1 mentions) Poly allylamine hydrochloride pah, by Merck Group (1 mentions) Triethanolamine teoa, by Merck Group (1 mentions) 2 amino 2 hydroxymethyl 1 3 propanediol trizma base, by Merck Group (1 mentions) Peg vs, by JenKem Technology (1 mentions) Poly sodium 4 styrenesulfonate, by Merck Group (1 mentions) Alginic acid sodium salt, by Merck Group (1 mentions) Pump 11 plus, by Harvard Apparatus (1 mentions) Tyramine, by Merck Group (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Corning (1 mentions) Dhr 3 rheometer, by TA Instruments (1 mentions) Diisopropylcarbodiimide, by Merck Group (1 mentions) Anhydrous dichloromethane, by Merck Group (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) 4 dimethylaminopyridine, by Merck Group (1 mentions) 5 norbornene 2 carboxylic acid, by Merck Group (1 mentions) 2 2 azobis 2 2 imidazolin 2 yl propane dihydrochloride va 044, by Fujifilm (1 mentions) Four arm peg, by JenKem Technology (1 mentions)

Spelling variants of this product

SH-PEG-COOH (3 citations) 8-arm-PEG-SH (2 citations)

7 protocols using peg sh

1

PEG Hydrogel Synthesis with Bioactive Peptides

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PEG hydrogels were prepared from 8-arm PEG maleimide (hexaglycerol) (PEG-MAL, 10 kDa, JenKem Technology) backbone and 8-arm PEG thiol (hexaglycerol) (PEG-SH, 10 kDa, JenKem Technology) crosslinker. RGD peptides (GCGYGRGDSSPG) for fibronectin, and HAVDI (HAVDIGGGC) or scrambled HAVDI control (AGVGDHIGC) peptides for N-cadherin (Sangon Biotech, Shanghai) were covalently conjugated to the PEG-MAL backbone via Michael addition reactions between the cysteine residues on these peptides and the maleimide on the PEG-MAL backbone. PEG-MAL (5 mM) and peptides were dissolved in phosphate-buffered saline (PBS) for 1 h at 37 °C for peptide conjugation. For these studies, all peptides were used at a final concentration of 1 mM in hydrogels (except for hydrogel control without any peptide conjugation).
Zhang C., Zhu H., Ren X., Gao B., Cheng B., Liu S., Sha B., Li Z., Zhang Z., Lv Y., Wang H., Guo H., Lu T.J., Xu F., Genin G.M, & Lin M. (2021). Mechanics-driven nuclear localization of YAP can be reversed by N-cadherin ligation in mesenchymal stem cells. Nature Communications, 12, 6229.
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2

Multifunctional Hydrogel Biosensor Fabrication

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PEG tetra-vinyl sulfone (10 kDa, PEG-VS) and PEG tetra-thiol (10 kDa, PEG-SH) purchased from JenKem Technology, USA. Oxygen-sensitive phosphor Pd(II) meso-tetra(sulfophenyl) tetrabenzoporphyrin sodium salt (S-PdBP) was purchased from Frontier Specialty Chemicals, USA. Glucose oxidase (≤ 40 U/mg) from Aspergillus niger, (Z,Z,Z)-Sorbitan tri-9-octadecenoate (SPAN 85), and polyoxyethylenesorbitan trioleate (TWEEN 85) were purchased from Tokyo Chemical Industry, USA. Catalase from bovine liver (2,000-5,000 U/mg), alginic acid sodium salt from brown algae (viscosity of ~250 cps at 2% solution, average Mw 75,000-100,000 Da), poly(allylamine hydrochloride) (PAH) (average Mw 17,500 Da), poly(Sodium 4-styrenesulfonate) (PSS) (average Mw ~70,000 Da), 2-amino-2-(hydroxymethyl)-1,3-propanediol (TRIZMA base), triethanolamine (TEOA), 2-(N-morpholino) ethane-sulfonic acid sodium salt (MES sodium salt), and calcium carbonate were purchased from Sigma Aldrich Chemical Co., St. Louis, MO, USA. Isooctane, dimethyl sulfoxide (DMSO), tris(hydroxymethyl) aminomethane (TRIS) hydrochloride, and phosphate buffered saline (PBS) tablets were purchased from Avantor Performance Materials, LLC, USA. Calcium chloride dihydrate was purchased from VWR Chemicals, LLC, OH, USA. All reagents were used as received without further purification unless mentioned otherwise.
Fahey T., Montgomery A.A, & Peters T.J. (2001). Randomized trial evaluating the framing of cardiovascular risk and its impact on blood pressure control [ISRCTN87597585]. BMC Health Services Research, 1, 10.
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3

Fabrication of Coaxial Vascular Grafts

Cited 1 time
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The apparatus used to obtain tubular grafts made of coaxial fibers was developed in-house. Our previous paper described the detailed procedure of vascular graft fabrication.12 (link) A high-voltage ES30P 10 W power supply (Gamma High Voltage Research, Ormond Beach, FL) and syringe pumps (Pump 11 Plus, Harvard Apparatus, Boston, MA) were used. The polymer solutions consisted of 1.8 or 3 wt % PCL and 3 wt % 4-arm PEG-NB 5 kDa (synthesized in-house). The solutions were prepared by dissolving PCL (for core solution) and PEG-NB 5 kDa, poly(ethylene glycol) dithiol (PEG-SH, JenKem Technology, Dallas, TX), PEO, and Irgacure 2959 (Ciba Speciality Chemicals, Basel, Switzerland) (for sheath solution) in the solvent 1,1,1,3,3,3 hexafluoro-2-propanol (HFP, CovaChem, Loves Park, IL). Using our custom-made electrospinning apparatus, the fibers were deposited onto a grounded aluminum rotating mandrel placed at a distance of 10 cm perpendicular to the needle. The mandrel was rotated at 350 rpm. The obtained samples were kept dry at room temperature until further use.
Iglesias-Echevarria M., Johnson R., Rafuse M., Ding Y, & Tan W. (2020). Vascular Grafts with Tailored Stiffness and a Ligand Environment via Multiarmed Polymer Sheath for Expeditious Regeneration. ACS applied bio materials, 4(1), 545-558.
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4

Hydrogel-based Triphasic Scaffold Fabrication

Cited 1 time
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The subset of hydrogels incorporated into triphasic scaffolds was selected from a previously characterized library of hydrogels (24 (link)). Four-arm PEG-thiol (PEG-SH) hydrogel precursor solutions were generated from mixtures of 4-arm PEG-thiol [PEG-SH; 20,000 weight-average molecular weight (Mw); JenKem Technology, Plano, TX] dissolved in Dulbecco’s phosphate-buffered saline (DPBS) (pH 7.3; Corning cellgro, Corning, New York) mixed with a reaction solution generated from H2O2 (30% solution, Macron, Radnor, PA), tyramine (99%, Sigma-Aldrich, St. Louis, MO), and HRP (Thermo Fisher Scientific, Waltham, MA). All samples contained a fixed amount of tyramine (5 mM) and HRP (5 U/ml). The polymer and reaction solutions were mixed to generate the polymerizing hydrogel solution. After mixing, samples were immediately loaded onto the bottom plate of a DHR-3 rheometer (TA Instruments, New Castle, DE) with a Peltier system controlling the temperature at 25 ± 0.1°C. A parallel plate fixture (20 mm diameter) was used with a measuring gap of 1000 μm. Oscillatory shear was continuously applied at 0.8 rad/s at a sampling interval of 30 s. Mixing and loading times were added to the start of the measurements to account for the short period of reaction before starting the measurement.
Sun Han Chang R.A., Shanley J.F., Kersh M.E, & Harley B.A. (2020). Tough and tunable scaffold-hydrogel composite biomaterial for soft-to-hard musculoskeletal tissue interfaces. Science Advances, 6(34), eabb6763.
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5

Synthetic Hydrogel for Tissue Engineering

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Four-arm PEG (MW = 20 kDa) (PEG-OH) and four-arm
poly(ethylene glycol)-thiol (MW = 10 kDa) (PEG-SH) were purchased
from JenKem Technology USA (Plano, Texas). Diisopropylcarbodiimide,
4-(dimethylamino)pyridine, 5-norbornene-2-carboxylic acid, and anhydrous
dichloromethane were purchased from Sigma-Aldrich (St. Louis, Missouri).
2,2′-Azobis[2-(2-imidazolin-2-yl) propane]dihydrochloride (VA-044)
was purchased from FUJIFILM Wako Pure Chemical Corporation (Richmond,
Virginia). 5-((2-(and-3)-S-(Acetylmercapto) succinoyl) amino) (SAMSA)
fluorescein was purchased from Fisher Scientific (Eugene, Oregon).
Minimum Essential Medium, penicillin–streptomycin, and GlutaMAX
were purchased from Fisher Scientific (Eugene, Oregon). SMP foams
were graciously provided by Shape Memory Medical, Inc. (Santa Clara,
California).
Jungmann M.A., Recalde Phillips S., Touchet T.J., Brinson B., Parish K., Petersen C., Hasan S.M., Nash L.D., Maitland D.J, & Alge D.L. (2023). Swellable and Thermally Responsive Hydrogel/Shape Memory Polymer Foam Composites for Sealing Lung Biopsy Tracts. ACS Biomaterials Science & Engineering, 9(2), 642-650.
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6

PEG Hydrogel Functionalization and Characterization

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PEG hydrogels were prepared by mixing 8-arm PEG maleimide (PEG-MAL, 10 kDa, JenKem Technology) and 8-arm PEG thiol (PEG-SH, 10 kDa, JenKem Technology) for 30 min at room temperature. To remove unreacted molecules, the hydrogels were rinsed thrice with PBS following each step. PEG hydrogels were treated with primary strand solution for 1 h for primary strand conjugation. Both of primary strand 1 and 2 were used at a final concentration of 500 μM in hydrogels. Then the primary strand-functionalized hydrogels were incubated with peptide-DNA solution for 1 h at room temperature to allow hybridization. FAM-labeled RGD and TAMRA-labeled HAVDI were used to characterize the conjugation of RGD or HAVDI in the hydrogels.
Zhang Z., Sha B., Zhao L., Zhang H., Feng J., Zhang C., Sun L., Luo M., Gao B., Guo H., Wang Z., Xu F., Lu T.J., Genin G.M, & Lin M. (2022). Programmable integrin and N-cadherin adhesive interactions modulate mechanosensing of mesenchymal stem cells by cofilin phosphorylation. Nature Communications, 13, 6854.
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7

Synthesis of Hyaluronic Acid-Based Hydrogel

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Sodium hyaluronate with molecular weight of 1.5 × 106 Da (HA, research grade) was purchased from LifeCore Biomedical Inc. (Chaska, MN, USA). Glycidyl acrylate (GA) was obtained from TCI America Inc. (Portland, OR, USA). The poly (ethylene glycol) dithiol (HS-PEG-SH) with an average MW of 5 kDa (PEG-SH, MW 5 kDa) was from JenKem Technology (Plano, TX, USA). All other chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise noted.
Yao Z.C., Yang Y.H., Kong J., Zhu Y., Li L., Chang C., Zhang C., Yin J., Chao J., Selaru F.M., Reddy S.K, & Mao H.Q. (2022). Biostimulatory Micro-fragmented Nanofiber-Hydrogel Composite Improves Mesenchymal Stem Cell Delivery and Soft Tissue Remodelling. Small (Weinheim an der Bergstrasse, Germany), 18(36), e2202309.
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