Log In Sign up
The largest database of trusted experimental protocols

Sybr green pcr mixture kits

Manufactured by Takara Bio
Visit Supplier
Sourced in United States

SYBR green PCR mixture kits are reagents used for quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The kits contain a pre-mixed solution with SYBR green dye, DNA polymerase, dNTPs, and buffer components necessary for the amplification and detection of DNA sequences.

Automatically generated - may contain errors

Lab products found in correlation

Cfx96 real time system, by Bio-Rad (2 mentions) Reverse transcriptase, by Qiagen (1 mentions) M mulv reverse transcriptase, by Qiagen (1 mentions)

Spelling variants of this product

SYBR Green (1328 citations) SYBR Green PCR kit (915 citations) SYBR Green kit (375 citations) SYBR Green Mixture (41 citations) SYBR mixture (16 citations) SYBR Green PCR mixture (3 citations) SYBR PCR mixture (2 citations) PCR Mixture (2 citations)

2 protocols using sybr green pcr mixture kits

1

Quantitative Analysis of miRNA and mRNA Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNAs were extracted using the RNeasy Mini Kit. First‐strand cDNAs were synthesized using Reverse Transcriptase (Qiagen). Quantitative PCRs (qPCRs) were performed in a CFX96TM Real‐Time System (Bio‐Rad), using SYBR green PCR mixture kits (Takara). miRNA qPCR Primers were used to determine miRNA expression levels. RNU6‐1 was used for miRNA template normalization. GAPDH was used as for gene expression endogenous control. Relative quantity of transcripts was calculated using the 2−ΔΔCt formula. All PCR primers are listed in Table 1. All data are presented as relative quantification.
Qin Y., Chen J., Xu K., Lu Y., Xu F, & Shi J. (2023). Triad3A involved in the regulation of endotoxin tolerance and mycobactericidal activity through the NFκB‐nitric oxide pathway. Immunity, Inflammation and Disease, 11(7), e925.
+ Open protocol
+ Expand
2

Quantitative PCR Analysis of Cellular RNAs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular RNAs were extracted using miRNeasy Mini Kit. First-strand cDNAs were synthesized using M-MuLV Reverse Transcriptase (Qiagen). Quantitative PCRs (qPCRs) were performed in a CFX96TM Real Time System (Bio-Rad, Hercules, CA, USA), using SYBR green PCR mixture kits (Takara, Shiga, Japan). Relative quantity of transcripts was calculated using the 2−ΔΔCt formula, as described previously. All PCR primers are reported in Table 1.
Qin Y., Wang Q., Zhou Y., Duan Y, & Gao Q. (2016). Inhibition of IFN-γ-Induced Nitric Oxide Dependent Antimycobacterial Activity by miR-155 and C/EBPβ. International Journal of Molecular Sciences, 17(4), 535.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!