Model l 7100

The amounts of main ingredients of GT were determined by HPLC (Model L-7100, Hitachi, Tokyo, Japan). Each extract was dissolved in methanol. The diluted samples were analyzed by an Ascentis^TM C18 column (No. 581325-U, 5 μm, 250 × 4.6 mm; Supelco, Bellefonte, PA, USA). The mobile phase was the solution of methanol/0.05% acetic acid in water (40:60, v/v). The flow rate was 1.0 mL/min. The concentration of the extract sample was 1000 μg/mL. The detection was conducted at 350 nm. The compounds used to build the calibration curves and applied in the cytotoxicity experiment on VSMCs were purchased from Sigma-Aldrich.

The HPLC system used in this study was a Hitachi Model L-7100 attached to a D-2500 Chromatopac data station and an ultraviolet (UV) detector. The detection wavelength used for adsorption and capacity evaluations was set at 259 nm in all cases. FTIR spectra with a resolution of 4 cm⁻¹ for analytes pelleted in potassium bromide (KBr) were acquired by scanning samples 10 times on a Shimadzu Model FTIR-8400 system. In all cases, a 15-cm C₁₈ column was used.