Rpmi dmem

Human CRC cell lines (SW480, SW620, LOVO, and HCT-116) and human normal colon epithelial cell line FHC were purchased from KeyGEN Company (Nanjing, China). SW480 and SW620 cells were cultured in Leibovitz’s L-15 (Gibco, Grand Island, NY) medium supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY), 1% penicillin–streptomycin (HyClone, Logan, Utah, USA), FHC, HCT-116 and LoVo cells were maintained in 90% RPMI DMEM (Gibco,Grand Island, NY) containing 10% FBS, 1% penicillin–streptomycin at 37 °C in air containing 5% CO₂.
Scrambled siRNA of SNHG7 or GALNT1 (siControl) and SNHG7 siRNAs (siSNHG7#1, siSNHG7#2 and siSNHG7#3), GALNT1 siRNA (siGALNT1), as well as pcDNA3.1-Control (pcDNA/Control), pcDNA3.1-SNHG7 (pcDNA/SNHG7) and pcDNA3.1-GALNT1 (pcDNA/GALNT1) were purchased from GenePharma (Shanghai, China). MiR-216b inhibitor, inhibitor negative control (NC inhibitor), miR-216b mimic and mimic negative control (NC mimic) were also purchased from GenePharma (Shanghai, China). Lipofectamine 2000 Reagents (Invitrogen Co., Carlsbad, CA, USA) were used for cell transfection. In vivo experiments, full-length SNHG7 cDNA subcloned into LV5 lentiviruses (GenePharma, Shanghai, China) and infection into SW480 cells to generate SW480/LV-SNHG7.

We obtained male wide-type C57BL/6 (WT; 6–9 weeks) mice from Shanghai Jihui Laboratory Animal Care (Shanghai, China). We maintained mice under a 12 h light/dark cycle with food and water available ad libitum. All procedures were approved by the Animal Care and Use Committee of Zhongshan Hospital, Fudan University (Shanghai, China) (approval code 202108006s). We performed all experiments in accordance with guidelines from the Chinese Animal Welfare Agency.
We maintained the murine microglial cell line BV-2 in culture media RPMI-DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS; Sigma Aldrich, Shanghai, China) and 1% penicillin–streptomycin solution at 37 °C and 5% CO₂ in a humidified incubator.

T-ALL cell lines Jurkat and TALL-1 were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS). Primary MSCs and MSC cell lines (MS-5 and HS-5) were cultured in DMEM medium supplemented with 10% FBS. 293T cells were cultured in RPMI-DMEM medium with 10% FBS. RPMI-1640, RPMI-DMEM and 0.25% trypsin-EDTA were obtained from Gibco.

Docetaxel and doxorubicin were purchased from Sigma (Selleck, Shanghai, China). Human TNBC HS578T cells were purchased from the American Type Culture Collection (ATCC, Shanghai, China). Cells were cultured in RPMI-DMEM (Gibco, USA) with 10% fetal bovine serum (FBS), 1% penicillin, and 1% streptomycin at 37 °C with 5% CO₂. The HS578T cells were seeded in 6-well culture plates at 2.5 × 10⁵ cells/well and were treated with 2 μM Docetaxel or doxorubicin for 24 h.