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Edwards sputter coater s150b

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The Edwards Sputter Coater S150B is a laboratory equipment designed for the deposition of thin films on various substrates. It utilizes a sputtering process to create uniform coatings on the target surface. The device features a compact design and is suitable for a range of applications that require thin film coatings.

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Lab products found in correlation

Z6 apo, by Leica (1 mentions) Quanta 250 feg, by Thermo Fisher Scientific (1 mentions) Jsm 5600lv, by JEOL (1 mentions) Balzers cpd 030, by Oerlikon Balzers (1 mentions) Glutaraldehyde, by Merck Group (1 mentions) Formaldehyde, by Merck Group (1 mentions) Evo ls15 scanning electron microscope, by Zeiss (1 mentions) Sodium cacodylate, by Merck Group (1 mentions)

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S150B sputter coater (39 citations) Sputter Coater (13 citations) Edwards sputter coater (8 citations)

6 protocols using edwards sputter coater s150b

1

Quantifying Adhesive Remnants on Enamel

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The amount of residual adhesive adhering to the enamel surface was quantified by using the adhesive remnant index (ARI) developed by Årtun and Bergland [33 (link)]. The ARI scores of all samples were recorded twice by the same investigator using an optical stereomicroscope (Leica Z 6 APO, Leica Microsystems, Wetzlar, Germany) under 10× magnification. Scoring groups are: 0, no adhesive remains on the tooth; 1, less than 50% of the adhesive remains on the tooth; 2, more than 50% of the adhesive remains on the tooth; 3, all adhesive remains on the tooth.
For scanning electron microscope (SEM) analysis of the adhesive remnants the samples were sputtered with gold/platinum in an Edwards sputter coater S150 B (Munich, Germany) and analyzed by SEM image (Phenom FEI G1 and Phenom Software Prosuite, Eindhoven, The Netherlands).
Blöcher S., Frankenberger R., Hellak A., Schauseil M., Roggendorf M.J, & Korbmacher-Steiner H.M. (2015). Effect on enamel shear bond strength of adding microsilver and nanosilver particles to the primer of an orthodontic adhesive. BMC Oral Health, 15, 42.
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2

Particle Size Analysis of Microcapsules

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The particle size distribution was determined according to the method previously reported [22 (link)] by laser diffraction spectroscopy on MasterSizer Microequipment (Malvern Panalytical, Malvern, United Kingdom). The microcapsules, suspended in deionized water, were sonicated in a bath for 2 min and immediately measured. De Broukere mean diameter (D4,3) or volume-weighted mean size was obtained. Photomicrographs of samples were obtained using a FEI scanning electron microscope (QUANTA 250 FEG, Hillsboro, OR, USA). Previously, samples were gold sputtered with an Edwards Sputter Coater S150B at 5 kV (Kings Grove Industrial Estate, United Kingdom).
Chasquibol N., Alarcón R., Gonzales B.F., Sotelo A., Landoni L., Gallardo G., García B, & Pérez-Camino M.C. (2022). Design of Functional Powdered Beverages Containing Co-Microcapsules of Sacha Inchi P. huayllabambana Oil and Antioxidant Extracts of Camu Camu and Mango Skins. Antioxidants, 11(8), 1420.
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3

Scaffold Characterization by SEM

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SEM was used to analyze the architectural features of the plain scaffolds and cell adhesion to the scaffold surfaces. Cellularized samples underwent preliminary fixation (4% neutral buffered formalin in PBS, overnight at 4°C), dehydration in a graded series of ethanol aqueous solutions up to anhydrous ethanol, drying by the critical point method (Balzers CPD030, Oerlikon Balzers, Balzers, Liechtenstein), and cross-sectioning. Samples were mounted on aluminum stumps, sputter-coated with gold (Edwards Sputter Coater S150B, Edwards, NY, USA) and examined with a scanning electron microscope (JEOL JSM-5600 LV, JEOL Ltd, Tokyo, Japan).
Ricci C., Mota C., Moscato S., D’Alessandro D., Ugel S., Sartoris S., Bronte V., Boggi U., Campani D., Funel N., Moroni L, & Danti S. (2014). Interfacing polymeric scaffolds with primary pancreatic ductal adenocarcinoma cells to develop 3D cancer models. Biomatter, 4, e955386.
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4

Scanning Electron Microscopy Sample Preparation

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The specimens were fixed in 2% OsO4 for 40 min and glued on small coverslips (snipped from 1 × 1 cm size to the size of stub) previously coated with Poly-L-Lysine and subjected to consecutive dehydration in an ethanol series. Samples were critical point dried according to Ref.181 (link). Later, these coverslips were fixed onto SEM stubs with carbon conductive tape. Finally, the samples were sputter-coated with gold (Edwards sputter coater S 150B) and analyzed with JSM-5410 scanning electron microscope181 (link).
Serra V., Gammuto L., Nitla V., Castelli M., Lanzoni O., Sassera D., Bandi C., Sandeep B.V., Verni F., Modeo L, & Petroni G. (2020). Morphology, ultrastructure, genomics, and phylogeny of Euplotes vanleeuwenhoeki sp. nov. and its ultra-reduced endosymbiont “Candidatus Pinguicoccus supinus” sp. nov.. Scientific Reports, 10, 20311.
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5

SEM Analysis of MLO-A5 Seeded PCL polyHIPE

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On day 28, the PCL polyHIPE discs seeded with MLO-A5s were washed 3 times with PBS and fixed with 2.5% glutaraldehyde at RT for 1 h and rinsed with PBS. Then the discs were soaked in deionised water for 5 min prior to dehydration of the samples with serial ethanol washes. Finally, HMDS is used as the chemical drying agent, and the discs were soaked in HMDS:ethanol (1:1) solution for 1 h and transferred into 100% HMDS for 5 min. The samples were then air-dried overnight in a fume hood and gold-coated at a current of 15 mA for 2.5 min with a gold sputter (Edwards sputter coater S150B, Crawley, England) prior to imaging under SEM (Philips/FEI XL-20 SEM; Cambridge, UK).
Aldemir Dikici B., Dikici S., Reilly G.C., MacNeil S, & Claeyssens F. (2019). A Novel Bilayer Polycaprolactone Membrane for Guided Bone Regeneration: Combining Electrospinning and Emulsion Templating. Materials, 12(16), 2643.
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6

Visualizing Abutment Epithelial Attachment

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Abutments were carefully removed from the RHG with tweezers to visualize epithelial keratinocyte attachment to the abutment surface. Abutments with epithelial layers were fixed in 1% glutaraldehyde (Merck KGaA, Darmstadt, Germany) and 4% formaldehyde (Merck KGaA) in 0.1 M sodium cacodylate (Merck KGaA) buffer for 2 to 3 days and postfixed in 1% osmium tetroxide for 2 hours. This procedure was followed by dehydration in a series of ascending ethanol concentrations at 50%, 70%, 90%, and 100% for 15 minutes each with two changes of each solution. Thereafter, the samples were sputter‐coated with gold using an Edwards Sputter Coater S150B (Edwards, Burgess Hill, England) and examined in a Zeiss EVO LS‐15 scanning electron microscope (Zeiss, Oberkochen, Germany).
Roffel S., Wu G., Nedeljkovic I., Meyer M., Razafiarison T, & Gibbs S. (2019). Evaluation of a novel oral mucosa in vitro implantation model for analysis of molecular interactions with dental abutment surfaces. Clinical Implant Dentistry and Related Research, 21(Suppl Suppl 1), 25-33.
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