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Axio observer z1 microscopy

Manufactured by Zeiss
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The Axio-Observer Z1 is a high-performance inverted microscope designed for a wide range of applications in biological and material sciences. It features a stable, vibration-resistant stand and a modular design that allows for the integration of various imaging techniques, including brightfield, phase contrast, differential interference contrast (DIC), and fluorescence microscopy.

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Lab products found in correlation

Lsm510 meta nlo, by Zeiss (1 mentions)

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Axio Observer Z1 (2173 citations) Axio Observer (1225 citations) Observer Z1 (439 citations) Axio Observer Microscopy (4 citations)

2 protocols using axio observer z1 microscopy

1

Time-lapse Microscopy of Cell Morphology

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Time-lapse images were captured using Axio-Observer Z1 microscopy (Zeiss) under incubating in PeCon microscopy incubator (PeCon GmbH) at 37°C and 5% CO2. The cell images were captured with 10 × objective in every 20 min for about 1.5 days to monitor cellular morphological change event.
Cho H.J., Yang E.J., Park J.T., Kim J.R., Kim E.C., Jung K.J., Park S.C, & Lee Y.S. (2020). Identification of SYK inhibitor, R406 as a novel senolytic agent. Aging (Albany NY), 12(9), 8221-8240.
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2

Quantitative Fluorescence Imaging Analysis

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Fluorescence images were acquired using Zeiss Axio Observer Z1 microscopy and AxioVision and Zeiss LSM 510 meta-NLO inverted confocal microscopy and Zen software. Images were acquired using identical parameters and quantified for fluorescence intensity. The fluorescence intensity was measured by using IPLab (Scanalytics). The ratio of fluorescence intensity of measured markers was normalized to total vasculature present using the molecular marker CD31 or ColIV. Image processing was performed to optimize intensity and color mixes visualization using Adobe Photoshop (Adobe Systems).
Li Q.F., Decker-Rockefeller B., Bajaj A, & Pumiglia K. (2018). Activation of Ras in the Vascular Endothelium Induces Brain Vascular Malformations and Hemorrhagic Stroke. Cell reports, 24(11).
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