Levels of tumor necrosis factor (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the rat hippocampus were analyzed using an enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer's instructions, with the optical density value measured using a microplate reader (DR-200Bs; DiaTek Company, Wuxi, China) at a wavelength of 450 nm. The following ELISA kits were used: Rat TNF-α ELISA Kit (ELK1396; ELK Biotechnology, Wuhan, China), Rat IL-1β ELISA Kit (ELK1272 ELK Biotechnology, Wuhan, China), and Rat IL-6 ELISA Kit (ELK5684; ELK Biotechnology, Wuhan, China).
Rat tnf α elisa kit
Manufactured by Elk Biotechnology
Sourced in China
The Rat TNF-α ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of rat tumor necrosis factor-alpha (TNF-α) in serum, plasma, and cell culture supernatants. The kit utilizes a specific antibody coated on a 96-well plate to capture rat TNF-α, and a detection antibody linked to an enzyme for colorimetric quantification.
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Lab products found in correlation
Rat il 6 elisa kit, by Elk Biotechnology (2 mentions)
Ab33922, by Abcam (1 mentions)
D gal, by Merck Group (1 mentions)
Fitc cona, by Merck Group (1 mentions)
Mda detection kit, by BestBio (1 mentions)
Tripure reagent, by Aidlab (1 mentions)
Mpo detection kit, by BestBio (1 mentions)
Primescript rt reagent kit, by Takara Bio (1 mentions)
Tb green premix ex taq 2, by Takara Bio (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Rneasy minelute cleanup kit, by Qiagen (1 mentions)
3 protocols using rat tnf α elisa kit
1
Quantification of Inflammatory Markers in Rat Hippocampus
2
Evaluating Inflammatory Markers in Rats
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D-gal was purchased from Sigma Aldrich (St Louis, MO, United States). ELISA kits (Rat TNF-α ELISA kit, Rat IL-6 ELISA kit, ELK Biotechnology, Wuhan, China) were used to detect TNF-α and IL-6 levels. Anti-BDNFs (ab108319), anti-GAPDH (ab37168), and anti-glial fibrillary acidic protein (GFAP) (ab33922) were purchased from Abcam (MA, United States). HRP goat anti-rabbit antibody (AS1107) was purchased from Aspen Biotechnology (Wuhan, China).
3
Comprehensive RNA Extraction and Analysis
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The RNA stabilization mix, RNeasy MinElute Cleanup kit was purchased from Qiagen (Hilden, Germany). TRIpure Reagent was purchased from Aidlab (Beijing, China). PrimeScript RT reagent kit and TB Green Premix Ex Taq II were purchased from TaKaRa (Dalian, China) and used according to the manufacturer recommendations. The other chemicals and reagents were of analytical grade and purchased from commercial sources unless otherwise stated. Propidium iodide, and FITC-ConA were purchased from Sigma-Aldrich (St. Louis, MO, USA.). Rat TNF-α ELISA Kit and Rat GM-CSF ELISA Kit were purchased from ELK Biotechnology (Wuhan, China). MPO Detection Kit and MDA Detection Kit were purchased from Bestbio (Shanghai, China).
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