G. citri-aurantii was inoculated in PDB medium and incubated at 180 rpm and 28 °C for 48 h. Then, DMDC solution was added to final concentrations of 0 and 250 mg/L. After incubation for another 24 h, the sample was collected and fixed with 2.5% (v/v) glutaraldehyde overnight. The following preparations were performed in the electron microscope laboratory. In detail, the samples were washed thrice with PBS, postfixed for 2 h in 1% osmic acid solution, and washed with PBS three times for 15 min. Then, they were dehydrated in an ethanol series (30%, 50%, 70%, 95%, and 100%, v/v) for 20 min, two times for each concentration. After drying, the samples were coated with gold films [24 (link)]. The mycelial morphology of G. citri-aurantii was observed using a JEOL JSM-6390LV SEM (JEOL, Tokyo, Japan).
Jsm 6390lv sem
Manufactured by JEOL
Sourced in Japan, United States
The JSM-6390LV is a Scanning Electron Microscope (SEM) manufactured by JEOL. It is a versatile tool used for high-resolution imaging and analysis of a wide range of samples. The JSM-6390LV utilizes an electron beam to scan the surface of a specimen, providing detailed information about its topography, composition, and other characteristics.
Automatically generated - may contain errors
Lab products found in correlation
H 7650 tem, by Hitachi (1 mentions)
Uc6 ultramicrotome, by Leica (1 mentions)
Cm1900, by Leica (1 mentions)
832 ccd camera, by Ametek (1 mentions)
H 7650, by Hitachi (1 mentions)
Jfc 1600, by JEOL (1 mentions)
Lambda 25, by PerkinElmer (1 mentions)
Rint2000 x ray diffractometer, by Rigaku (1 mentions)
Hcp 2 critical point dryer, by Hitachi (1 mentions)
Szx10, by Olympus (1 mentions)
Light microscopy, by Zeiss (1 mentions)
Mz16f dissecting microscope, by Leica (1 mentions)
Dcf300 fx, by Leica (1 mentions)
Q150t es, by Quorum Technologies (1 mentions)
Jsm 5500lv, by JEOL (1 mentions)
Hcp 2, by Hitachi (1 mentions)
21 protocols using jsm 6390lv sem
1
Mycelial Morphology of Geotrichum citri-aurantii
2
Structural Analysis of Bacterial Proteins
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All purified proteins were precentrifuged for 10 min at 16,000 × g at 4°C. Proteins (0.6 μM each) were incubated with 2 mM GTP and/or 1 mM ATP for 10 min at 37°C in buffer P (Text S1 ) to observe the structure formation of MsmK and its derivatives. For observing FtsZ polymerization in vitro, mixtures of 0.6 μM FtsZ and/or His-tagged MsmK constructs (0.6 μM each) were incubated at 37°C for 2 min in buffer P to analyze FtsZ polymerization in vitro. Afterward, 2 mM GTP and/or 1 mM ATP was added into the mixtures, which were continuously incubated for 10 min at 37°C. Mixtures were then withdrawn and applied to glow-discharged carbon-coated grids. The grids were air-dried and visualized using an H-7650 TEM (Hitachi, Japan).
The cells in TSB were grown to an OD600 of 0.5 and fixed overnight with 2.5% glutaraldehyde at 4°C to observe the bacterial morphology under TEM. For SEM, the cell cultures were grown in TSB to an OD600 of 0.5 and spotted onto poly-l -lysine-coated coverslips, followed by washing with PBS buffer. The cells were fixed overnight with 2.5% glutaraldehyde at 4°C. Subsequent dehydration steps were performed using ethanol as previously described (19 (link)). The dried samples were covered with a 10-nm-thick layer of gold and observed with a JSM-6390LV SEM (JEOL, Japan).
The cells in TSB were grown to an OD600 of 0.5 and fixed overnight with 2.5% glutaraldehyde at 4°C to observe the bacterial morphology under TEM. For SEM, the cell cultures were grown in TSB to an OD600 of 0.5 and spotted onto poly-
3
Scanning Electron Microscopy of Cotton Fiber Initiation
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For SEM, flowers of YZ1 were picked from the same positions in the afternoon at 0 DPA, and the ovules were cultured for 1 day (24 h) in BT medium with Control and ERI treatments. After 1 day, the ovules were washed with ddH2O, immediately fixed in 2.5% glutaraldehyde and stored at 4 °C for 24 h. Thereafter, the samples were rinsed through an ethanol series, transferred to isoamyl-acetate and air dried. Photographs were taken with a JSM-6390/LV SEM (Jeol, Tokyo, Japan) to visualize fibre initials.
4
Microscopic Analysis of Hongkong Kumquat Plastids
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Protoplasts from the calli were generated as previously described [69 ], then protoplast suspensions were dropped onto microscope slides to observe the plastid modes. Light microscopy of various orange tissues of Hongkong kumquats was performed using a frozen sectioning technique with a Leica CM1900 (Leica, Germany). An optical microscope (BX61, Olympus) equipped with a DP70 camera was used in tandem with a differential interference contrast (DIC) technique.
Transmission electron microscopy (TEM) analysis was performed according to Cao et al. [28 (link)]. Samples were prepared using a normal fixation process with 2.5% glutaraldehyde adjusted to pH 7.4, and a 0.1 M phosphate buffer with 2% OsO4. The preparations were dehydrated and embedded in epoxy resin and SPI-812, respectively. Ultrathin sections obtained with a Leica UC6 ultramicrotome were stained with uranyl acetate and subsequently with lead citrate. Image recording was performed with a HITACHI H-7650 transmission electron microscope at 80 KV and a Gatan 832 CCD camera.
Starch granule morphology was examined with a scanning electron microscope (SEM). The samples were mounted on studs, sputter coated with gold (Balzers, JFC-1600), and examined under a JSM-6390LV SEM (JEOL, Japan).
Transmission electron microscopy (TEM) analysis was performed according to Cao et al. [28 (link)]. Samples were prepared using a normal fixation process with 2.5% glutaraldehyde adjusted to pH 7.4, and a 0.1 M phosphate buffer with 2% OsO4. The preparations were dehydrated and embedded in epoxy resin and SPI-812, respectively. Ultrathin sections obtained with a Leica UC6 ultramicrotome were stained with uranyl acetate and subsequently with lead citrate. Image recording was performed with a HITACHI H-7650 transmission electron microscope at 80 KV and a Gatan 832 CCD camera.
Starch granule morphology was examined with a scanning electron microscope (SEM). The samples were mounted on studs, sputter coated with gold (Balzers, JFC-1600), and examined under a JSM-6390LV SEM (JEOL, Japan).
5
Floral UV Patterns and Longevity
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Ray petals from flowers of each plant host (HETU N = 18, VEAL N = 9) were collected from a site near Panther Hollow Pond in Schenley Park, Pittsburgh, PA (40.436983, −79.946868) between September 24 and October 26, 2018. Petals were pressed and dried for photographic analysis of the pattern of UV following Koski and Ashman (2013 ). For each petal, we recorded length, width, total area, and total UV‐absorbing area using ImageJ. We marked 5 transects (“T1–5”; see Figure 1e ) and calculated the proportion of UV absorbing area at the base (area within T1 + T2) and tip (area within T4 + T5) and calculated the average for each host species.
Floral longevity was monitored in the laboratory. Closed flower heads (N = 8–10 per host) were observed and scored as “open” when at least one ray petal was completely unfurled and considered “senesced” when the last ray petal was shed or completely shriveled.
Scanning electron microscopy was used to visualize the petal cell shape at the tip and base of ray petals from both hosts. Petals were collected in the field and immediately imaged at the University of Pittsburgh with a JEOL JSM6390LV SEM at 350X without fixing, dehydrating, or sputter‐coating using an ion beam with a 7kv accelerating voltage. Charging was mitigated by viewing in the low vacuum using 22–28 Pascals.
Floral longevity was monitored in the laboratory. Closed flower heads (N = 8–10 per host) were observed and scored as “open” when at least one ray petal was completely unfurled and considered “senesced” when the last ray petal was shed or completely shriveled.
Scanning electron microscopy was used to visualize the petal cell shape at the tip and base of ray petals from both hosts. Petals were collected in the field and immediately imaged at the University of Pittsburgh with a JEOL JSM6390LV SEM at 350X without fixing, dehydrating, or sputter‐coating using an ion beam with a 7kv accelerating voltage. Charging was mitigated by viewing in the low vacuum using 22–28 Pascals.
6
Characterization of Biosynthesized Ag/AgCl Nanoparticles
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The reduction of silver ions to Ag/AgCl NPs was monitored by using a UV-Vis spectrophotometer (Perkin-Elmer Lambda 25, Boston, MA, USA) from 250 to 800 nm. Subsequently, Fourier transform infrared radiation (Perkin-Elmer Universal ATR 100) was employed to identify the characteristic vibrational frequency of Ag/AgCl NPs [14 (link)]. Particle size and shape of Ag/AgCl NPs were determined using Transmission electron microscope (JEOL 1210, Austin, TX, USA), operated at an accelerated voltage of 100 kV. The surface morphology of nanoparticles was studied with SEM (JEOL JSM-6390 LVSEM) and the elemental compositions of Ag/AgCl NPs was carried out with Noran Six 200 Energy Dispersive X-ray (JEOL Ltd., Tokyo, Japan) [14 (link)]. XRD analysis was carried out by using X-ray Diffractometer to determine the crystalline nature of the biosynthesized Ag/AgCl NPs.
7
Cryo-Fractured SEM Imaging
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SEM imaging was performed using JEOL JSM-6390LV SEM (JEOL USA Inc., Peabody, MA, USA) operated at 25 keV. To examine the internal structure, the samples were cryo-fractured using liquid nitrogen. Prior to analysis, the samples were coated with a conducting layer of gold.
8
Microstructural Analysis of Sewage Sludge and Sugarcane Bagasse
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The micro- and macro-structure of raw and torrefied sewage sludge and sugarcane bagasse were examined using scanning electron microscopy (JSM-6390LV, SEM) (JEOL, Austin, TX, USA). Prior to the analysis and mounting of the sample on a stub of metal, the samples were coated with a thin layer of material, specifically gold, for high-resolution electron imaging. The SEM examinations were performed in a high vacuum mode using an accelerating voltage of 15.0 kV. The high-energy electrons from the SEM then interact with different atoms in the sample to produce various signals that reveal information on the surface morphology of the examined samples.
9
Characterization of Prepared Samples
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All the prepared samples were characterized by using Scanning Electron Microscopy (SEM) JSM-6390LV SEM from JEOL equipped with Inka-act Energy Dispersive X-ray Analysis (EDX) system from Oxford and X-ray Diffraction (XRD) using using a Rigaku RINT-2000 X-ray diffractometer. XRD patterns were recorded from 30° to 70° 2θ angles using CuKα1 radiation with a monochromatic wavelength of 1.5405 Å operated at 40 kV and 82 mA. Photoluminescence (PL) spectroscopy using a He-Cd, cw laser at 325 nm with full power 35 mW was also performed. The samples were placed in a high vacuum cryostat, which was cooled down to change the temperature from 300 K to 13 K. The emission spectrum was measured using a very sensitive, LN2 cooled CCD camera.
10
Beef Muscle Nanostructure Analysis
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The 20 g LTL samples which were harvested for the muscle nanostructure were immediately put in small bottles containing 3% formalin for fixation. They were then transported to the Botany laboratory of the University of Fort Hare for approximately 2 hours in cooler box filled with ice. During the analysis, the samples were dehydrated to remove formalin and kept in ethanol for 20 minutes in an ascending order of 10% up to 100% respectively. In order to improve electrical conductivity of the sample surface in the scanning electron microscope (SEM), a thin film of gold palladium was used for sputter coating to enhance the analysis.
Critical point drying was performed using the Hitachi critical point dryer HCP-2 (Hitachi Koki Co Ltd, Tokyo, Japan) to prevent the samples from alteration and to boost good structural preservation. This was done by mounting the samples on aluminium stubs with double-sided carbon tape then the sputter coating with gold-palladium (Au-Pb) using the Eiko IB.3 Ion Coater (EIKO Engineering Co TD, Tokyo, Japan). The samples were then observed under the JEOL JSM-6390LV SEM for the determination of the skeletal surface area of beef muscles. The nanostructure of the skeletal surface area for the samples was then viewed using JEOL JM-5600 SEM at ×5,000 magnification on 0, 1, 3, and 7 days aged meat samples.
Critical point drying was performed using the Hitachi critical point dryer HCP-2 (Hitachi Koki Co Ltd, Tokyo, Japan) to prevent the samples from alteration and to boost good structural preservation. This was done by mounting the samples on aluminium stubs with double-sided carbon tape then the sputter coating with gold-palladium (Au-Pb) using the Eiko IB.3 Ion Coater (EIKO Engineering Co TD, Tokyo, Japan). The samples were then observed under the JEOL JSM-6390LV SEM for the determination of the skeletal surface area of beef muscles. The nanostructure of the skeletal surface area for the samples was then viewed using JEOL JM-5600 SEM at ×5,000 magnification on 0, 1, 3, and 7 days aged meat samples.
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