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Annexin 5 fluorescein isothiocyanate fitc propidium iodide kit

Manufactured by MultiSciences Biotech
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The Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) kit is a laboratory tool used for the detection and quantification of apoptosis and necrosis in cells. The kit contains Annexin V-FITC, which binds to phosphatidylserine exposed on the surface of apoptotic cells, and propidium iodide, which stains the nuclei of necrotic cells. This combination allows the identification and differentiation of viable, apoptotic, and necrotic cell populations through flow cytometry analysis.

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Lab products found in correlation

Flow cytometry, by BD (1 mentions) Fc500 flow cytometer, by Beckman Coulter (1 mentions) Cell cycle staining kit, by MultiSciences Biotech (1 mentions)

Spelling variants of this product

Propidium iodide (51 citations) Annexin V-FITC (43 citations) Annexin V (13 citations) Annexin V-fluorescein isothiocyanate (FITC) (2 citations)

2 protocols using annexin 5 fluorescein isothiocyanate fitc propidium iodide kit

1

Annexin V-FITC/PI Cell Apoptosis Assay

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Cells were stained with the annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) kit (Multisciences). The detailed procedures refer to the manufacturer’s instruction and previous research (Li et al., 2018 (link)). Cell apoptosis was analyzed by using flow cytometry (Becton Dickinson).
Ran J., Li J., Yin L., Zhang D., Yu C., Du H., Jiang X., Yang C, & Liu Y. (2021). Comparative Analysis of Skeletal Muscle DNA Methylation and Transcriptome of the Chicken Embryo at Different Developmental Stages. Frontiers in Physiology, 12, 697121.
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2

Apoptosis and Cell Cycle Analysis

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Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) kit (MULTI SCIENCE, Hangzhou, China) and cell cycle staining kit (MULTI SCIENCE, Hangzhou, China) were used to analyze the apoptotic rates and cell cycle profiles, respectively. The percentage of apoptotic cells and the distribution of cells in different phases of cell cycle were analyzed by flow cytometry (FC500 flow cytometer, Beckman-Coulter, USA).
Yao Y., Ma W., Guo Y., Liu Y., Xia P., Wu X., Chen Y., Wang K., Mei C., Wang G., Li X., Zhang Z., Chen X, & Yuan Y. (2022). USP53 plays an antitumor role in hepatocellular carcinoma through deubiquitination of cytochrome c. Oncogenesis, 11(1), 31.
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